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Phlebopus portentosus mother culture preserving method

A technology of black boletus and mycelium, which can be applied in the fields of botanical equipment and methods, fertilizer mixtures, gardening, etc., and can solve the problems of difficult preservation of strains

Active Publication Date: 2015-06-17
贵州宏臻菌业投资发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] One of the purposes of the present invention is to provide a method for preserving the mother species of black boletus which is easy to operate and can maintain the vigor of the strain for a long time, so as to solve the problem that its strains are difficult to preserve in the cultivation and scientific research of black boletus, and provide a new method for other cattle liver. A reference for the preservation of hepatic and mycorrhizal edible fungi

Method used

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  • Phlebopus portentosus mother culture preserving method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0090] (1) Cultivate the mother species of Boletus nigricans with the improved medium formula. The formula and preparation method of the mother species medium are as follows:

[0091] a: Culture medium formula

[0092] Red loam 100 g

[0093] Glucose 10.0 g

[0094] Yeast Extract 1.0g

[0095] NH 4 NO 3 1.0g

[0096] CaCl 2 0.5g

[0097] ZnSO 4 0.5g

[0098] FeSO 4 0.5g

[0099] Vitamin B 1 100.0ug

[0100] Agar powder 15.0 g

[0101] water 1000ml

[0102] pH 4.5~6.5

[0103] b : preparation method

[0104] Take 1000 ml of water, put it into red loam, boil for 5 minutes, then filter, take the filtrate, add water to make up to 1000 ml, add the rest of the ingredients in the formula, pour it into a test tube with a specification of 25×200, and the filling amount is 20 ml per tube , sterilized at 121°C for 20 minutes, and then placed on a slope.

[0105] (2) Cultivation of black boletus parent species: collect wild bolet...

Embodiment 2-3

[0110] As a preference, the preparation of the mother seed medium, the cultivation of the mother seed, the production of the mother seed preservation storage tube and the storage temperature, the vitality of the mother seed and the inspection of the cultivation traits in Example 2-3 are the same as those in Example 1, and the differences are shown in Table 1.

[0111] Table 1

[0112]

Embodiment 4

[0114] As a preference, the formulation and preparation of the mother seed culture medium, the cultivation of the mother seed, and the production of the mother seed storage tube are the same as in Example 2, except that the storage temperature is 13°C.

[0115] Mother species viability and cultivation traits inspection: Activate the black boletus strains stored at 13°C for 2 half years, multiply and cultivate them into liquid strains, and inoculate the liquid strains into the bagged substrate to artificially cultivate black boletus, test Strain activity and cultivation traits, the results: a. The mycelium of the strain multiplication grows fast, and the activated mycelium is used to inoculate the slant medium in the test tube, and the slant is covered with mycelia 28 days after inoculation; b. The mycelium is inoculated into the liquid medium to cultivate the liquid strains. Mycelium begins to form on the 3rd day after the inoculation. By the 9th day, the mycelium is golden in ...

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Abstract

The invention relates to a phlebopus portentosus mother culture preserving method. The method comprises the steps that mycelium is cultivated on a solid medium, sclertium is extracted to be placed in a container filled with sterile purified water, the container is sealed, and the sclertium is preserved at the constant temperature ranging from 10 DEG C to 15 DEG C. The invention further relates to the culture medium for preservation of the phlebopus portentosus mother culture and the purpose of the culture medium in cultivating and / or preserving of the culture medium.

Description

technical field [0001] The invention relates to a method for preserving the mother species of black boletus, belonging to the technical field of edible fungus cultivation. Background technique [0002] black boletus ( Phlebopus portentosus ), also known as the dark brown reticulum boletus, is a precious and most popular edible fungus in tropical areas, and it is also a member of the Boletaceae ( Boletaceae ) Pueraria spp. ( Phlebopus ) is the only boletus that can be cultivated by saprophytic cultivation methods, and it is distributed in Sri Lanka, Vietnam, Indonesia, Thailand, Brazil, Mexico, Australia, New Zealand and my country's Yunnan, Guangxi and Hainan. Black boletus is fresh and tender in quality, delicious in taste, rich in nutritional value, high in protein and low in fat, rich in 17 kinds of essential amino acids and mineral elements necessary for human body such as phosphorus, potassium, calcium, magnesium, iron and zinc. Good health food and has good medici...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04C05G3/00
Inventor 石建同
Owner 贵州宏臻菌业投资发展有限公司