Open-type culture method of microalgae

An open culture and open technology, applied in the field of open culture of microalgae, can solve the problem of promoting the growth of algae and the accumulation of oil in microalgae cells

Active Publication Date: 2014-05-07
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The above-mentioned prior art is to control the growth balance of algae in the water body through bacteria, not to promote the growth of algae and the accumulation of oil in microalgae cells

Method used

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  • Open-type culture method of microalgae

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Embodiment Construction

[0021] The method for cultivating microalgae in the open mode of the present invention specifically includes the following content: before the semi-mixed culture, the two kinds of microorganisms are separately cultured in seed liquid, and a stirring bioreactor is used to cultivate the seed liquid of facultative anaerobic bacteria. The autotrophic microalgae seed liquid culture is carried out in the device, the stirred bioreactor includes facultative anaerobic bacteria and anaerobic bacteria fermentation medium, and the photobioreactor includes microalgae and self-supporting SE medium. After the seed liquid culture is qualified, it is transferred to the lower area of ​​the open photobioreactor with the internal membrane separation device for cultivating facultative anaerobic bacteria and the upper area of ​​the photobioreactor for cultivating microalgae cells through their respective inoculation lines. And add the aseptically treated mixed medium nutrients and acid-base neutrali...

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Abstract

The invention discloses an open-type culture method of microalgae. The method includes the steps of: (1) cultivating a facultative anaerobic bacteria seed liquid; (2) cultivating an autotrophic microalgae seed liquid; and (3) inoculating the facultative anaerobic bacteria seed liquid and the autotrophic microalgae seed liquid into an open-type photobioreactor with a built-in membrane separation device, which divides the photobioreactor into an upper zone and a lower zone, to carry out semi-mixing culture, fermenting the anaerobic bacteria in the zone below the membrane separation device, and subjecting the autotrophic microalgae to photosynthesis growth in the zone above the membrane separation device, wherein the medium composition and culture conditions are the same in the two zones, and supplementing organic carbon source required by the growth and metabolism of the facultative anaerobic bacteria in the culture process. The method has the advantages of increasing the yield of autotrophic microalgae, the utilization rate of an inorganic carbon source (CO2), and oil content of the microorganisms, simplifying the culture device and improving the efficiency of large scale culture of autotrophic microalgae.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to an open method for cultivating microalgae, in particular to an open semi-mixed culture of autotrophic oil-producing microalgae and facultative anaerobic bacteria to obtain high microalgae cell harvest, while improving A method for the lipid content of microalgal cells. Background technique [0002] In today's world, oil resources are becoming increasingly scarce, the contradiction between supply and demand of oil products is becoming more and more prominent, and the problem of environmental pollution is also becoming more prominent. It is inevitable to develop alternative resources. Diesel derived from renewable resources such as microbial oil has high energy density, low sulfur content, sufficient combustion, and good lubricity. It is also renewable, environmentally friendly, easy to biodegrade, safe for storage and transportation, and good antiknock performance And other characteris...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N1/20C12R1/89C12R1/07C12R1/01C12R1/225C12R1/22
Inventor 王领民金平廖莎吴丹李晓姝师文静张霖高大成乔凯程国香
Owner CHINA PETROLEUM & CHEM CORP
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