Technology for simultaneously detecting ten estrogens in water environment
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A technology of estrogen and water environment, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problem of few types of estrogen, and achieve the effects of good reproducibility, pollution reduction, and low detection limit
Active Publication Date: 2014-05-14
厦门中科育成中心管理有限公司
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There are many methods to detect estrogen, but there are few types of estrogen that can be detected at the same time
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[0028] Below in conjunction with example and accompanying drawing, the present invention will be further described:
[0029] (1) Water sample pretreatment
[0030] The collected water samples were filtered through a filter membrane with a pore size of 0.45 μm and the pH value was adjusted to 3.0. Measure 1.0 L of water sample, and enrich the water sample with C18 (6 mL, 200 mg) solid-phase extraction cartridge from Waters Company, and the speed is controlled at 10 ml / min. The solid-phase extraction cartridge was activated before use under the condition that it was pretreated with 6 mL of ethyl acetate, 6 mL of methanol and 6 mL of aqueous hydrochloric acid at pH=3. After the enrichment was completed, the column was washed with 6 mL of 10% (v / v) methanol aqueous solution to remove other impurities, and then vacuum-dried for 20 min to remove water. After drying, the target compound was eluted with 6 mL of ethyl acetate into a nitrogen blowpipe and blown dry with a gentle strea...
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Abstract
The invention relates to a technology for simultaneously detecting ten estrogens in water environment. The technology is characterized in that a water sample is pre-processed through a solid-phase extraction technology, and a liquid chromatogram tandem mass spectrometer is utilized to detect the content. The technology comprises the following steps: performing microfiltration membrane on a collected water sample to remove contamination particles suspending in water, concentrating for enriching estrogens in the water sample to a solid phase extraction column through a solid-phase extraction device, eluting the estrogens enriched on the solid phase extraction column by ethyl acetate, drying by using nitrogen, making constant volume, and detecting the content of the estrogens by adopting liquid chromatogram tandem mass spectrometer. The method provided by the invention has the advantages that the operation is simple, the recovery rate is high, the time consumption is low, and the multiple estrogens are simultaneously detected.
Description
technical field [0001] The invention relates to a liquid chromatography tandem mass spectrometer for the simultaneous determination of estrone, 17β-estradiol, estriol, ethinyl estradiol, nonylphenol, octylphenol, estradiol valerate, hexyl Ten estrogen methods such as diethylstilbestrol, diethylstilbestrol, and bisphenol A. Background technique [0002] Environmental estrogen is an exogenous substance that can simulate and interfere with the normal synthesis, transportation, and combination of estrogen in humans or animals, and can affect the reproductive system, immune system, and reproductive system of the body. Estrogens present in the environment mainly include natural estrogens such as estrone (Estrone, E1), 17β-estradiol (17β-Estradiol, E2) and estriol (Estriol, E3) and synthetic estrogens such as ethinyl estradiol Alcohol (17α-Ethynylestradiol, EE2), nonylphenol (4-n-Nonylphenol, NP), octylphenol (4-tert-Octylphenol, OP), estradiol valerate (17-beta-Estradiol 17-valer...
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