Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Technology for simultaneously detecting ten estrogens in water environment

A technology of estrogen and water environment, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problem of few types of estrogen, and achieve the effects of good reproducibility, pollution reduction, and low detection limit

Active Publication Date: 2014-05-14
厦门中科育成中心管理有限公司
View PDF6 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many methods to detect estrogen, but there are few types of estrogen that can be detected at the same time

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Technology for simultaneously detecting ten estrogens in water environment
  • Technology for simultaneously detecting ten estrogens in water environment
  • Technology for simultaneously detecting ten estrogens in water environment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0028] Below in conjunction with example and accompanying drawing, the present invention will be further described:

[0029] (1) Water sample pretreatment

[0030] The collected water samples were filtered through a filter membrane with a pore size of 0.45 μm and the pH value was adjusted to 3.0. Measure 1.0 L of water sample, and enrich the water sample with C18 (6 mL, 200 mg) solid-phase extraction cartridge from Waters Company, and the speed is controlled at 10 ml / min. The solid-phase extraction cartridge was activated before use under the condition that it was pretreated with 6 mL of ethyl acetate, 6 mL of methanol and 6 mL of aqueous hydrochloric acid at pH=3. After the enrichment was completed, the column was washed with 6 mL of 10% (v / v) methanol aqueous solution to remove other impurities, and then vacuum-dried for 20 min to remove water. After drying, the target compound was eluted with 6 mL of ethyl acetate into a nitrogen blowpipe and blown dry with a gentle strea...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a technology for simultaneously detecting ten estrogens in water environment. The technology is characterized in that a water sample is pre-processed through a solid-phase extraction technology, and a liquid chromatogram tandem mass spectrometer is utilized to detect the content. The technology comprises the following steps: performing microfiltration membrane on a collected water sample to remove contamination particles suspending in water, concentrating for enriching estrogens in the water sample to a solid phase extraction column through a solid-phase extraction device, eluting the estrogens enriched on the solid phase extraction column by ethyl acetate, drying by using nitrogen, making constant volume, and detecting the content of the estrogens by adopting liquid chromatogram tandem mass spectrometer. The method provided by the invention has the advantages that the operation is simple, the recovery rate is high, the time consumption is low, and the multiple estrogens are simultaneously detected.

Description

technical field [0001] The invention relates to a liquid chromatography tandem mass spectrometer for the simultaneous determination of estrone, 17β-estradiol, estriol, ethinyl estradiol, nonylphenol, octylphenol, estradiol valerate, hexyl Ten estrogen methods such as diethylstilbestrol, diethylstilbestrol, and bisphenol A. Background technique [0002] Environmental estrogen is an exogenous substance that can simulate and interfere with the normal synthesis, transportation, and combination of estrogen in humans or animals, and can affect the reproductive system, immune system, and reproductive system of the body. Estrogens present in the environment mainly include natural estrogens such as estrone (Estrone, E1), 17β-estradiol (17β-Estradiol, E2) and estriol (Estriol, E3) and synthetic estrogens such as ethinyl estradiol Alcohol (17α-Ethynylestradiol, EE2), nonylphenol (4-n-Nonylphenol, NP), octylphenol (4-tert-Octylphenol, OP), estradiol valerate (17-beta-Estradiol 17-valer...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
Inventor 罗专溪王大鹏张娴颜昌宙林立峰
Owner 厦门中科育成中心管理有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com