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Quality test method for penthorum Chinense pursh

A quality inspection method and technology of the inspection method are applied in the field of quality inspection of Huangcao, which can solve problems such as evaluation and effective quality control of difficult-to-catch Huangmao materials and their preparations, and achieve easy quality control, high separation, and exclusive use. strong effect

Active Publication Date: 2014-05-28
四川天植中药股份有限公司
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  • Application Information

AI Technical Summary

Problems solved by technology

However, for many years, there has been no revision of "Hunan Province Traditional Chinese Medicinal Materials Standards" to revise the item of "Huanhuangcao", and the standard mainly uses the content of quercetin as the standard, so it is difficult to conduct a comprehensive evaluation and effective quality control of the medicinal materials and their preparations.

Method used

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  • Quality test method for penthorum Chinense pursh
  • Quality test method for penthorum Chinense pursh
  • Quality test method for penthorum Chinense pursh

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The content of the total flavonoids in the yellow grass of embodiment 1UV method determination

[0032] 1 Preparation of solution

[0033] Preparation of rutin reference substance solution Accurately weigh 10.6mg of rutin in a 10mL beaker, dissolve it with 50% ethanol, and set the volume in a 100mL volumetric flask, the concentration is 0.106mg·mL -1 .

[0034] Preparation of the test solution Precisely weigh about 0.1g of the fine powder into a 50mL conical flask with a stopper, add 10mL of 70% ethanol solution to each of the 5 conical flasks at a ratio of 100:1, shake well, seal, and avoid Stand in the light for 1h, ultrasonically extract in an ultrasonic cleaner for 1h, shake well, let stand to cool, and take the subsequent filtrate.

[0035] 2 Selection of measurement method and measurement wavelength

[0036] Take 0.2mL each of the rutin reference solution and the test solution in a 5mL volumetric flask, add water to the mark, shake well, add 0.1mol·mL -1 Alumi...

Embodiment 2

[0063] Embodiment 2 The selection of the method for measuring the color development of total flavonoids by ultraviolet spectrophotometry of the present invention

[0064] Flavonoids (Flavonoids) are based on C 6 -C 3 –C 6 The derivatives of chromane or chromone, whose structure is the basic core, are favored for their wide range of pharmacological effects. The content determination of total flavonoids in Chinese medicinal materials has become the focus of research on flavonoids in recent years. The colorimetric method used in the determination of total flavonoids in traditional Chinese medicine is the most common method for the determination of total flavonoids adopted by the "Chinese Pharmacopoeia". Among them, NaNO 2 -Al(NO 3 ) 3 -NaOH colorimetry is commonly used. This method uses NaNO 2 -Al(NO 3 ) 3 It is a chromogenic agent, and under alkaline conditions, it is characterized by the formation of red aluminum chelates with flavonoids. Taking rutin as a control, the...

Embodiment 3

[0076] The TLC identification of embodiment 3 quercetin

[0077] Take 2.0g of C. chinensis fine powder, add 50mL of 70% ethanol, ultrasonically extract for 40min, filter, evaporate the filtrate to dryness, add 20mL of water to dissolve the residue, extract twice with petroleum ether, 20mL each time, discard the petroleum ether, add water to Dilute hydrochloric acid 20mL, put it in a water bath at 100°C for 1h, take it out, cool it rapidly, shake and extract twice with ethyl acetate, 20mL each time, combine the ethyl acetate solution, wash with 30mL water, discard the water washing solution, ethyl acetate The liquid was evaporated to dryness, and the residue was dissolved in 10 mL of ethyl acetate, filtered, and used as the test solution [13] . Another quercetin reference substance was dissolved in ethyl acetate to make a reference solution containing 0.5 mg per 1 mL. Take 10 μL each of the above-mentioned test sample and reference solution, spot the sample on the same silica...

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Abstract

The invention provides a quality test method for penthorum Chinense pursh. The method simultaneously tests the contents of general flavone and quercetin in the penthorum Chinense pursh so as to effectively control the quality of the penthorum Chinense pursh. The method simultaneously tests the general flavone and quercetin in the penthorum Chinense pursh so as to effectively control the quality of the penthorum Chinense pursh and extract, so that the test method has the advantages of high specificity, high degree of separation and stability, and can facilitate control over the quality of products in laboratories and large-scale industrial production.

Description

technical field [0001] The invention relates to a method for detecting the quality of yellow grass. Background technique [0002] C. chinense Pursh is the dry aerial part of Penthorum chinense Pursh, a plant of the saxifrage family C. chinense Pursh. It mainly contains flavonoids, volatile oil and other ingredients. Among them, gallic acid and quercetin have been identified as the ingredients known to have anti-hepatitis B virus and liver protection effects, and are the active ingredients of the yellow grass. According to the existing literature, there are only research reports on its chemical components, and clinical reports on the use of Gansu Granules, a Chinese patent medicine containing this medicinal material, for the treatment of hepatitis and anti-hepatic fibrosis. However, for many years, there has been no amendment to the item of "Hunan Province Traditional Chinese Medicinal Materials" and the standard mainly uses the content of quercetin as the standard, so it i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33G01N30/02G01N30/90
Inventor 税丕先朱烨欧丽兰李婷余昕张春庄元春李建卢娟
Owner 四川天植中药股份有限公司
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