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Method for directly detecting chloramphenicol in honey by using ND-EESI-MS (neutral desorption-extractive electrospray ionization mass spectrometry)

A neutral desorption and chloramphenicol technology, applied in the detection field, can solve the problems of cumbersome operation steps, time-consuming, inconvenient detection of chloramphenicol, and achieve high desorption efficiency and improved strength

Inactive Publication Date: 2014-05-28
NANCHANG UNIV
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  • Application Information

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Problems solved by technology

These methods generally require a complex sample pretreatment process, which has the disadvantages of cumbersome operation steps and time-consuming, which brings a lot of inconvenience to the detection of chloramphenicol in honey.

Method used

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  • Method for directly detecting chloramphenicol in honey by using ND-EESI-MS (neutral desorption-extractive electrospray ionization mass spectrometry)
  • Method for directly detecting chloramphenicol in honey by using ND-EESI-MS (neutral desorption-extractive electrospray ionization mass spectrometry)
  • Method for directly detecting chloramphenicol in honey by using ND-EESI-MS (neutral desorption-extractive electrospray ionization mass spectrometry)

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Experimental program
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Embodiment 2

[0032] 1. Adding standard chloramphenicol honey treatment: prepare 1.0 mg / mL chloramphenicol standard solution with methanol to make 1.0 mg / mL chloramphenicol standard solution for later use; accurately measure 1 mL honey sample, add chloramphenicol standard Dilute the solution with 20 mL of methanol: water (1:1) to prepare a chloramphenicol honey solution with a concentration gradient of 100-8000 ng / mL, add a stirring bar and stir on a stirrer at room temperature for 3 min, measure 7 mL Add honey diluent to a 10 mL Erlenmeyer flask for experimentation.

[0033] 2. Sample preparation: Take 1 mL of the honey to be tested, add 20 mL of methanol: water diluent with a volume ratio of 1:1 to dilute, and take 7 mL into a 10 mL Erlenmeyer flask.

[0034] 3. Neutral desorption-electrospray extraction ionization mass spectrometry (ND-EESI-MS):

[0035] (1) Set the ND-EESI ion source to negative ion mode, the mass range is 50-500 Da, the neutral desorption spray reagent is methanol, th...

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Abstract

The invention belongs to the field of analysis and testing, and discloses a method for directly detecting chloramphenicol in honey by using ND-EESI-MS (neutral desorption-extractive electrospray ionization mass spectrometry). The method comprises the following steps: (1) preparing marked honey; (2) preparing a sample; (3) detecting by using the ND-EESI-MS; (4) selecting the secondary characteristic fragment ions m / z257 of m / z321 to perform quantitative analysis so as to obtain the amount of chloramphenicol. By using the method, the chloramphenicol in the honey can be rapidly detected and identified without pretreatment or chromatographic separation on the sample to be tested, and the defects that in the prior art, the detection steps are complex, and a long time is consumed are overcome.

Description

technical field [0001] The invention belongs to the technical field of detection, in particular to a detection method for chloramphenicol in honey. Background technique [0002] China is the largest beekeeping country in the world, as well as a major producer and exporter of bee products. During the production process, beekeepers used chloramphenicol-containing drugs when disinfecting and treating diseases in bee colonies, and bees carried chloramphenicol in the environment when collecting pollen, resulting in chloramphenicol residues in honey. Chloramphenicol (Chloramphenicol, CAP) is a high-efficiency broad-spectrum antibiotic that has been used for many years. Chloramphenicol residues in food can cause serious damage to the hematopoietic function of human bone marrow, thereby causing human agranulocytosis, aplastic anemia and hemolytic anemia, and severe cases can be fatal. Antibiotics prohibited for use in food-producing animals. With the improvement of people's livin...

Claims

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Application Information

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IPC IPC(8): G01N27/68G01N1/28
Inventor 罗丽萍陈焕文郭夏丽张茜方小伟戴喜末
Owner NANCHANG UNIV
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