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Method for preparing alpha-glucanase by vitue of chaetomium gracile fermentation under ultrasonic condition

A technology of Chaetomyces clamosa and dextranase, which is applied in the field of microbiology and biology, can solve the problems of low production of glucanase and difficulty in reaching the level of industrial application, and achieve the effect of increasing enzyme activity and increasing growth rate

Inactive Publication Date: 2014-06-18
GUANGXI UNIV
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Problems solved by technology

At present, there are few domestic studies on the production of α-glucanase by the fermentation of Chaetomyces spp., and the main research focuses on the method and steps of preparing α-glucanase crude enzyme by using Chaetomyces spp. The output is low, it is difficult to reach the level of industrial application

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  • Method for preparing alpha-glucanase by vitue of chaetomium gracile fermentation under ultrasonic condition
  • Method for preparing alpha-glucanase by vitue of chaetomium gracile fermentation under ultrasonic condition
  • Method for preparing alpha-glucanase by vitue of chaetomium gracile fermentation under ultrasonic condition

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Embodiment Construction

[0015] The method of the present invention will be described in further detail below in conjunction with the accompanying drawings and specific embodiments.

[0016] 1. Fermentation medium composition and culture conditions: 1.5% dextran, 1.0% yeast extract, 0.5% dipotassium hydrogen phosphate (potassium dihydrogen phosphate), 0.1% magnesium sulfate. Use 1mol / L sodium hydroxide solution to adjust the pH of the medium to 6.0, sterilize, add a certain amount of calcium carbonate (sterilized) to the medium, and insert the fine hair shells into 25mL medium with a 2% inoculation amount , cultivated at 30° C. for 3-4 days in a shaker with a rotating speed of 200 rpm.

[0017] Ultrasonic treatment conditions: Ultrasonic treatment starts after 12 hours of fermentation, the frequency is 30KHZ, the power is 100W, the frequency of ultrasonic treatment is 1 hour / time, and the time of each treatment is 1min. Then put it into a shaker for cultivation, and the cultivation conditions are the...

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Abstract

The invention discloses a method for preparing an alpha-glucanase by virtue of chaetomium gracile fermentation under an ultrasonic condition. The method is characterized in that when the chaetomium gracile is cultured in a culture medium, an ultrasonic action having the frequency of 20-40KHZ and the power of 80-120W is applied by the frequency of 40-80 minutes each time for 40-80 seconds each time. Beyond the expectation of the technicist in the field, the method for preparing the alpha-glucanase by virtue of chaetomium gracile fermentation under the low-frequency and low-power ultrasonic condition increases the growth rate of cells, and meanwhile, the enzyme activity of the glucanase is improved by 215%.

Description

technical field [0001] The invention belongs to the technical field of microbes and biology, in particular, it relates to a method for preparing alpha-glucanase by fermentation of Chaetomium ciliatus under ultrasonic conditions. Background technique [0002] Dextran, also known as dextran, is a polysaccharide that exists in the mucus secreted by some microorganisms during their growth. Dextran has a relatively high molecular weight, and is mainly connected by D-glucopyranose with α, 1→6 bonds, and the branch points are connected by 1→2, 1→3, 1→4. With the different types of microorganisms and growth conditions, the structure of dextran is also different. During the production process of refined sugar, microorganisms such as Leuconostoc enterococcus entrained in sugarcane will secrete sucrose invertase to consume sucrose and produce a large amount of glucan, commonly known as "cane rice". In addition to the loss of sugar, the accumulation of dextran also leads to a series o...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/46C12N13/00C12R1/645
CPCC12N9/2454C12N13/00C12Y302/01011
Inventor 刘继栋李凯杭方学陆海勤谢彩锋梁欣泉陆登俊张泽栋
Owner GUANGXI UNIV
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