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A special compound enzyme for suckling pig containing probiotics and preparation method thereof

A composite enzyme, suckling pig technology, applied in microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of low β-glucanase enzyme activity, poor thermal stability, etc., and achieve a wide range of pH values. , improve the resistance to infection, improve the utilization of protein and energy

Active Publication Date: 2016-06-29
HUNAN HONGYING BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, a large number of production practices and scientific experiments have also proved that the use of enzyme preparations such as β-glucanase can effectively solve this problem, but the enzyme activity of β-glucanase currently used in production and experiments Mostly low and poor thermal stability

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The preparation method of embodiment 1 Chinese herbal medicine powder

[0038] A preparation method of Chinese herbal medicine powder is as follows:

[0039] Weigh 10 parts of Radix Astragali; 15 parts of Codonopsis; 12 parts of Radix Radix;

Embodiment 2

[0040] Embodiment 2 A kind of preparation method of bacillus licheniformis culture

[0041] A preparation method for a bacillus licheniformis culture, comprising the steps of:

[0042] (1) Expanded cultivation of liquid seeds

[0043] ①First-level seed culture: put the activated slant strains into 500 ml shake flasks with 2 rings, the medium capacity is 100 ml, the rotary shaker is 180 rpm, the culture temperature is 40°C, and the culture time is 10 hours;

[0044] ②Cultivation of secondary seeds: Inoculate 10% of primary seeds into 500 ml secondary seed shake flasks, and the cultivation conditions are the same as primary seeds;

[0045] ③Third-level seed cultivation: Inoculate the second-level seeds with 10% inoculum amount into a 5000 ml third-level seed shaker flask, the medium capacity is 1000 ml, the rotary shaker is 100 rpm, the culture temperature is 40°C, and the culture time is 12 hours ;

[0046] ④First-level seed tank cultivation: Put the third-level seeds into t...

Embodiment 3

[0055] Embodiment 3 A kind of preparation method of the special compound enzyme for suckling piglet containing probiotics

[0056] A special compound enzyme for suckling piglets containing probiotics, consisting of the following enzyme preparations in parts by weight:

[0057] 10 parts of Chinese herbal medicine powder, 1000 parts of xylanase, 100 parts of cellulase, 275 parts of Bacillus licheniformis culture, 20 parts of mannanase, 800 parts of protease, 65 parts of mesophilic α-amylase.

[0058] Get xylanase, cellulase, mannanase, mesophilic α-amylase, protease, and the Chinese herbal medicine powder gained from Hunan Hongyingxiang Biological Engineering Co., Ltd. according to the above parts by weight, and implement The Bacillus licheniformis culture described in Example 2 was stirred evenly in a mixer at 25° C., and the stirring time was 5 minutes. Stir evenly and store in a box at room temperature or in a cold room.

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Abstract

The invention relates to a probiotics-containing special enzyme for piglets and a preparation method of the special enzyme, and belongs to the field of feed enzyme preparations. The probiotics-containing special enzyme for the piglets comprises the following components in parts by weight: 5-20 parts of Chinese herbal medicine powder, 800-1200 parts of xylanase, 80-120 parts of cellulase, 250-300 parts of bacillus licheniformis culture, 15-25 parts of mannose, 600-1000 parts of protease and 50-80 parts of medium-temperature alpha-amylase, wherein the bacillus licheniformis culture is bacillus licheniformis beta-10-25; the preservation number of the bacillus licheniformis culture is CCTCCNO.M2013538. The preparation method of the probiotics-containing special enzyme for the piglets comprises the following steps: fermenting bacillus licheniformis, then filtering, concentrating and drying the bacillus licheniformis to obtain a culture, compounding the culture with other components. The complex enzyme is capable of improving the feed conversion rate, reducing the feed cost and improving preservation stability.

Description

technical field [0001] The invention belongs to the field of enzyme preparations, and in particular relates to a special compound enzyme for suckling piglets containing probiotics and a preparation method thereof. Background technique [0002] In animal husbandry, suckling pig rearing is the most complex of all stages of pigs. The consequences of unreasonable nutrient supply are slow growth of pigs, high diarrhea rate and high mortality rate, which in turn slows down the growth of medium and large pigs and prolongs the slaughter time. [0003] However, in order to cut off the vertical transmission of maternal diseases, shorten the reproductive cycle of sows and prolong the breeding life, people have widely used early weaning technology in the large-scale pig breeding industry. However, due to the lack of innate immunity of newborn piglets, the development of the digestive system is not perfect, and the ability to secrete digestive enzymes is weak, direct supply of feed main...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K50/30A23K20/189A23K20/10A23K10/18C12N1/20C12R1/10
Inventor 李海清胡永明胡波周玉明易继云
Owner HUNAN HONGYING BIOTECHNOLOGY CO LTD
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