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A method for producing docosahexaenoic acid using Schizochytrium

A technology of docosahexaenoic acid and Schizochytrium, which is applied in the field of fermentation, can solve the problems of increasing income and high energy consumption, and achieve the effects of increasing biomass, increasing production, and optimizing process parameters

Inactive Publication Date: 2016-08-17
WUHAN HUASHITE IND BIOTECH DEV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the current method of producing DHA using Schizochytrium is DHA, and the DHA content does not exceed 50%. Further increasing the DHA content will greatly improve product quality, thereby greatly increasing revenue without increasing costs.
At the same time, in order to maintain the normal growth of bacteria in the existing technology, it is necessary to maintain a large amount of ventilation in the fermenter, which consumes a lot of energy

Method used

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  • A method for producing docosahexaenoic acid using Schizochytrium
  • A method for producing docosahexaenoic acid using Schizochytrium

Examples

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Effect test

Embodiment 1

[0044] A method for producing docosahexaenoic acid by using Schizochytrium, including the following steps:

[0045] (1) The Schizochytrium sp S056 was activated and cultivated into a seed solution of Schizochytrium sp.

[0046] (2) Connect the seed liquid of Schizochytrium to the fermentation tank at a volume ratio of 2% for cultivation. The culture temperature is 28 degrees Celsius. The aeration is maintained at a constant speed and the aeration rate is 10 cubic meters per hour to 50 cubic meters per hour. The supplement includes a carbon source, a nitrogen source, and glycerin to maintain a glycerin concentration of 0 to 10 g / L. The carbon source is glucose, and the nitrogen source is yeast powder. Feeding feed, divided into three feedings, each feeding, when the carbon source concentration in the fermentation broth drops to 20.0 g / l to 30.0 g / l, start feeding, when the carbon source concentration in the fermentation broth is 30.0 g / L to 40.0 g / L, the nitrogen source concentra...

Embodiment 2

[0056] A method for producing docosahexaenoic acid by using Schizochytrium, includes the following steps:

[0057] (1) Activate and cultivate Schizochytrium into a seed solution of Schizochytrium. The Schizochytrium was obtained according to the following method: take a certain amount of water sample and spread it on a high-salt YPD solid medium (glucose 20.0g / L, yeast powder 10.0g / L, peptone 20.0g / L , Agar 20.0g / L, sea crystal 10.0g / L), cultured at 25.0 degrees Celsius for 48 hours to obtain a large number of single colonies. Pick the single colonies in the liquid medium, rotate at 180 rpm on the shaker, and culture at 25.0 degrees Celsius. After 72 hours of shaking flask culture under the conditions of, the bacteria were stained and observed under microscope, and the strains with high oil production were screened. Gas chromatograph was used to analyze the fatty acid composition of the oil of the above strains. A strain containing DHA with a higher content was further identifie...

Embodiment 3

[0068] A method for producing docosahexaenoic acid by using Schizochytrium, including the following steps:

[0069] (1) Activate and cultivate Schizochytrium into Schizochytrium seed liquid. The Schizochytrium was obtained according to the following method:

[0070] The Schizochytrium bacteria collected in Example 2 were subjected to multiple ultraviolet-nitrosoguanidine compound mutagenesis. The mutagenesis method was to prepare a suspension of Schizochytrium bacteria using 0.5 mL of PBS buffer (100 mM, pH 7.0). Add 0.5mL2.5mg / mL nitrosoguanidine solution and treat for 1 hour at 30 degrees Celsius; centrifuge the above treatment solution under aseptic conditions, wash the bacteria with distilled water to make the OD 650 After reaching 0.4 to 0.6, pour the bacterial solution into a thin layer of liquid with a thickness of 1mm to 5mm, and then irradiate it under a 25W ultraviolet lamp at a distance of 40mm for 30 seconds, and centrifuge under aseptic conditions. The bacteria were r...

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Abstract

The invention discloses a method for producing a docosahexaenoic acid (DHA) by using schizochytrium limacinum. The method comprises the following steps of (1) activating and culturing schizochytrium limacinum into a schizochytrium limacinum seed solution; (2) introducing the schizochytrium limacinum seed solution into a fermentation tank for culturing under the culture condition of 25-28 DEG C, aerating and stirring at a constant rotation speed, meanwhile fluidly feeding materials including carbon sources, nitrogen sources and glycerin, and keeping the concentration of glycerin at 0-10.0 g / liter; (3) controlling the temperature of a fermentation stable period at 18-23 DEG C, aerating and stirring at a constant rotation speed; and (4) when the concentration of the carbon source in a fermentation liquor is less than 5.0 g / liter, completing fermentation and collecting wet cells to obtain docosahexaenoic acid-rich greases. After the method is applied to large-scale fermentation and production of docosahexaenoic acid, the biomass of schizochytrium limacinum and the DHA content can increase, the quality of a DHA grease product is greatly improved, the grease quality is further improved and the energy consumption and the production cost are reduced.

Description

Technical field [0001] The invention belongs to the field of fermentation, and more specifically, relates to a method for producing docosahexaenoic acid by using Schizochytrium. Background technique [0002] Docosahexaenoic acid (DHA) is a polyunsaturated fatty acid and has very important physiological functions. It is an important nutrient that the human body cannot synthesize in large quantities but is indispensable. DHA exists in large amounts in the nerve tissues of the human retina and cerebral cortex. It can promote brain and vision development, improve intelligence, and improve memory and vision. In addition, it can prevent cholesterol from depositing on blood vessel walls and prevent or reduce atherosclerosis. Like sclerosis and coronary heart disease. [0003] The traditional production method of DHA mainly comes from the extraction and purification of deep-sea fish oil, and deep-sea fish oil resources are very limited, the production cost is high, the quality is unstable...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64C12R1/645
Inventor 余龙江朱圆敏陈伟周蓬蓬金文闻余金龙钱凯
Owner WUHAN HUASHITE IND BIOTECH DEV