A method for preventing gene drift of transgenic cotton by using nectar source plants

Abstract

The method relates to an effective method for measuring a pollen gene drift distance of a transgenic cotton, and the method is simple and practical, and has strong maneuverability. A sterile line of cotton used in production is planted at different positions surrounding the transgenic cotton, to form a drift region of transgenic cotton pollens. If the sterile line of cotton sends up boll and goes to seed normally, it can be confirmed as outcrossing; and then the rate of transgenic cotton is further analyzed. The method is easy for assessment of pollination rate and pollination source of cotton, avoids high frequencies of field monitoring and reduces the workload of cotton leaf DNA extraction and exogenous gene detection in laboratory. The method has the main characteristics of fastness, accuracy and strong operability.

Description

Technical field: [0001] The invention relates to an effective biological isolation method for preventing genetic drift of transgenic cotton through pollen biological transmission. Background technique: [0002] With the rapid development of biotechnology, transgenic crops have made great achievements. Statistics show that since the large-scale cultivation of genetically modified crops began in 1996, it has maintained a relatively rapid growth momentum. By 2009, the acreage of GM crops in 25 countries around the world had exceeded 134 million hectares. Currently, the United States is the country with the largest planting area of ​​GM crops in the world, followed by Brazil and Argentina. 77% of the world's soybean production comes from genetically modified crops, 48% of the world's cotton production is genetically modified, and the proportions of genetically modified corn and canola exceed 25% and 20%, respectively. Transgenic plants have attracted increasing attention beca...

AI Technical Summary

Problems solved by technology

[0003] The possible harm caused by transgenic plants to the ecological environment: First, it breaks the ecological balance, and the recombined genes may lead to unexpected results and produce harmful new biological types

The second is genetic pollution. The genes carried by transgenic plants are transmitted to wild species or other related species through pollen, which may cause the proliferation of certain weeds or produce a new poisonous weed, which is the so-called weedization. For example, insect-resistant transgenic plants pass The pollen transmits foreign genes to its relatives, making the plant insect-resistant, which may be toxic to some insects that depend on it for survival

However, the above method is only suitable for small-scale field release tests of transgenic crops. For large-scale commercial production, it is impractical to use high-stalk crops to set up isolation zones and other physical isolation to prevent the spread of transgenes through pollen, and other countermeasures must be considered

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