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A kind of nucleic acid aptamer of docetaxel, nucleic acid aptamer derivative and use thereof

A nucleic acid aptamer and docetaxel technology, which can be applied to preparations for in vivo experiments, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve the problem of no nucleic acid aptamers found , to achieve the effects of avoiding adverse drug reactions, prolonging drug efficacy, and shortening the sequence length

Active Publication Date: 2016-08-31
HUNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, no nucleic acid aptamer with high affinity and low cost to docetaxel has been found

Method used

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  • A kind of nucleic acid aptamer of docetaxel, nucleic acid aptamer derivative and use thereof
  • A kind of nucleic acid aptamer of docetaxel, nucleic acid aptamer derivative and use thereof
  • A kind of nucleic acid aptamer of docetaxel, nucleic acid aptamer derivative and use thereof

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Embodiment

[0035] A docetaxel nucleic acid aptamer of the present invention, the nucleotide sequence of the nucleic acid aptamer includes a DNA fragment shown in the following sequence, that is, a characteristic sequence capable of specifically binding docetaxel:

[0036] 5'-TTCCAAACGTCCACCGCCAAAA-3'

[0037] (Can also be 5'-AAAACCGCCACCTGCAAACCTT-3').

[0038] The nucleotide sequence of the above-mentioned nucleic acid aptamer is selected from naturally occurring or artificially synthesized sequences, or the same sequences from any other sources.

[0039] A nucleic acid aptamer sequence of docetaxel, the sequence of the nucleic acid aptamer contains all the same sequences as the nucleotides of the above-mentioned characteristic sequence.

[0040] A certain position on the nucleotide sequence of the nucleic acid aptamer can be phosphorylated, oxymethylated, methylated, aminated, sulfhydrylated or isotoped.

[0041] The nucleotide sequence of the above-mentioned nucleic acid aptamer can...

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Abstract

The invention discloses an aptamer of docetaxel, an aptamer derivative and use thereof. The aptamer sequence of the docetaxel comprises a DNA segment shown in a sequence 1 or a sequence 2. The aptamer can be the derivative obtained from various similar sequences with high homology or the sequence disclosed by the invention. The aptamer and the derivative thereof disclosed by the invention can be used as drug carriers, or applied to drug design and development, drug separation and purification, preparation of drugs and other products, preparation of docetaxel detection probes or target probes and the like. Therefore, the aptamer and the derivative thereof have the advantages that the aptamer and the derivative thereof can be combined with high specificity and high affinity of the docetaxel, can be subjected to chemical synthesis, and is free of immunogenicity, good in biocompatibility, small and stable in molecular weight, easy to store and the like.

Description

technical field [0001] The present invention relates to a nucleic acid aptamer, nucleic acid aptamer derivatives and uses thereof, in particular to a nucleic acid aptamer capable of binding docetaxel, nucleic acid aptamer derivatives and uses thereof. Background technique [0002] Docetaxel is a taxane antineoplastic drug, which mainly interferes with the microtubule network required during cell mitosis and division, and has an antitumor effect. Its adverse reactions are mainly allergic reactions, thrombocytopenia, and tachycardia , hypotension, neurotoxicity, gastrointestinal tract and skin toxicity. Docetaxel has low water solubility, and the commercially available drug Taxotere is solubilized by Tween-80 and dissolved by ethanol, which may cause side effects such as allergic reactions and hemolytic reactions, and there are potential risks in clinical application. Therefore, how to reduce the risk of taxotere Side effects and improvement of clinical curative effect have b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/115A61K47/26A61K49/00G01N21/64A61K31/337
Inventor 羊小海王柯敏陈南迪王青朱莹
Owner HUNAN UNIV
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