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Gene detection kit for prognostic evaluation of lung cancer, as well as preparation method and applications thereof

A detection kit and gene technology are applied to the gene detection kit used for the prognosis evaluation of lung cancer and the fields of preparation and application thereof, which can solve the problem of lack of mature products and methods for gene detection closely related to the prognosis of lung cancer, etc. Highly effective and sensitive effects

Inactive Publication Date: 2014-07-02
BEIJING XIPUSHENG INT BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are no mature products and methods for the detection of genes closely related to the prognosis of lung cancer

Method used

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  • Gene detection kit for prognostic evaluation of lung cancer, as well as preparation method and applications thereof
  • Gene detection kit for prognostic evaluation of lung cancer, as well as preparation method and applications thereof
  • Gene detection kit for prognostic evaluation of lung cancer, as well as preparation method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] PCR amplification of 5 genes in tumor tissue samples of lung cancer patients

[0057] 1-2 grams of tumor tissue from a lung cancer patient after surgery (with informed consent signed by the patient), and the DNA in the tumor tissue of the patient was extracted according to the method of DNA extraction and purification of human tumor tissue. The extracted DNA is the template for multiplex PCR amplification, and the 1% (m / v) agarose gel dissolved by heating with 1×TAE buffer is used for electrophoresis to detect the DNA quality; and 2 μl is diluted 50 times with 1×TAE buffer , DNA quantification was performed using a nucleic acid quantifier (Bio-Rad, USA).

[0058] After obtaining the DNA template of the lung cancer tumor tissue sample, multiplex PCR amplification was performed to enrich the target gene. Multiplex PCR amplification reaction conditions are as follows:

[0059] 1) PCR reaction system:

[0060]

[0061] The amplification reaction system for the 5 genes...

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Abstract

The invention provides a gene detection kit for prognostic evaluation of lung cancer. The gene detection kit comprises multiple DNA polymerase chain reaction (PCR) amplification primer sequences of five genes closely relevant to prognosis of the lung cancer, and chips for fixing five gene probes, as well as a human tumor tissue DNA extraction solution, a PCR amplification reaction solution, a negative control solution, a positive control solution and a DNA hybridization solution. The invention further provides a preparation method of the kit. The expression level of the five genes closely relevant to the prognosis of the lung cancer can be fast, effectively, accurately and economically detected by detecting the expression of the genes closely relevant to the prognosis of the lung cancer through adopting a surface Plasmon resonance instrument with both high sensitivity and specificity, and the kit can be used for detecting the clinical prognosis evolution and clinical guidance pharmacy of the lung cancer.

Description

technical field [0001] The invention relates to a detection kit for the expression of related genes in the clinical prognosis evaluation of lung cancer, as well as the preparation of the kit and its use in the clinical prognosis evaluation and clinical guidance of drug use in lung cancer. The five genes closely related to the prognosis of lung cancer involved in the present invention are protein kinase C iota, five-transmembrane single-chain glycoprotein Prominin1, acetaldehyde dehydrogenase 1, breast cancer drug resistance protein ATP-binding transporter G superfamily member 2 It binds to transcription factor 4 with octamer, and can be used to evaluate the prognosis of lung cancer and guide the clinical medication of patients. Background technique [0002] At present, lung cancer is a common malignant tumor in humans, which seriously threatens human life. With the progression of the disease, the clinical prognosis of lung cancer patients is constantly changing. At differe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6837C12Q1/686C12Q2537/143C12Q2565/501C12Q2565/628
Inventor 黄启明李一民徐世洪李一汉陈宗明连日平
Owner BEIJING XIPUSHENG INT BIOTECH
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