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A nitrate-reducing bacteria and its application

A technology of nitrate, cctccno.m2013573, applied in the direction of bacteria, microorganisms, drilling compositions, etc., can solve the problems of low viscosity retention rate, large loss of polymer injection viscosity, etc., and achieve the effect of improving the action efficiency

Active Publication Date: 2016-07-13
黑龙江吉纳森生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problems of high polymer injection viscosity loss and low viscosity retention rate in the oil production process, and provides a strain of nitrate reducing bacteria and its application

Method used

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  • A nitrate-reducing bacteria and its application
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  • A nitrate-reducing bacteria and its application

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specific Embodiment approach 1

[0026] Embodiment 1: A nitrate-reducing bacterium in this embodiment is Alccligenessp.JNS05, which is preserved in the China Center for Typical Culture Collection, and the preservation address is Wuhan University, Wuhan, China. The preservation date is November 14, 2013, and the preservation number is It is CCTCC No.M2013573.

[0027] In this embodiment, Alccligenessp.JNS05 is enriched and cultured by using sewage from the No. 3 Oil Production Plant of Daqing Oilfield as an environmental sample to enrich and cultivate Alccligenessp.JNS05. The colony is light yellow and opaque with smooth edges. Total DNA was extracted from Alccligenessp.JNS05 using standard methods. To amplify the 16S rRNA gene by PCR, the general primer F27 of the 16S rRNA gene: 5'-AGAGTTTGATCATGGCTCAG-3' and the primer R1492: 5'-TACGGTTACCTTGTTACGACTT-3' were used. The PCR amplification products were directly subjected to bidirectional sequencing after purification. The 16SrRNA gene sequence was submitted ...

specific Embodiment approach 2

[0029] Embodiment 2: The application of a strain of nitrate-reducing bacteria in this embodiment refers to the application of Alccligenessp.JNS05 in inhibiting the viscosity loss of oil recovery polymers.

[0030] The usage of Alccligenessp.JNS05 in this embodiment is: add 0.025% Alccligenessp.JNS05 culture solution of the volume of the polymer solution to the polymer solution for oil displacement, and add once every 24 hours.

[0031] Alccligenessp.JNS05 culture fluid in the present embodiment refers to that Alccligenessp.JNS05 is cultivated to OD in the culture medium 620 =1.8 The obtained culture solution.

[0032] This embodiment has the following advantages:

[0033] (1) This embodiment can co-exist in the microbial film with harmful bacteria, and can directly contact with harmful bacteria, thereby improving the action efficiency;

[0034] (2) The active ingredients in this embodiment can fully adapt to the anaerobic environment in the pipeline, and will not be graduall...

specific Embodiment approach 3

[0036] Specific Embodiment Three: The screening method of Alccligenesssp.JNS05 in this embodiment is as follows: use the injection sewage of the third oil production plant of Daqing Oilfield as an environmental sample to enrich and cultivate nitrate reducing bacteria. First, collect 50ml of the injection sewage sample of the third oil production plant, and add the The culture medium was cultured at 30°C, and after static culture for 72 hours, the enriched culture solution was transferred to a solid selection medium for plate separation and screening. The single colonies were transferred to the liquid selective medium again, and the obtained strains were tested for nitrate reduction performance using Griess reagent and diphenylamine reagent to screen positive clones. After three rounds of screening, a single positive nitrate-reducing bacteria strain JNS05 was obtained.

[0037] The formula of the selection medium of the present embodiment is: NaCl7.0g / L; MgSO 4 ·H 2 O0.68g / L;...

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Abstract

The invention relates to a nitrate reducing bacterium and applications thereof, aiming at solving the problems that during oil production process, the injection viscosity loss of polymer is large and the viscosity retention rate is lower. The nitrate reducing bacterium is Alccligenes sp.JNS05, and preserved at 14th, November, 2013, in China Center for Type Culture Collection (CCTCC) in Wuhan University in Wuhan city, China, with the preservation number of CCTCC No.M2013573. The application is to inhibit the viscosity loss of the oil production polymer. The compatibility effect of the Alccligenes sp.JNS05 and polymer working solution is excellent, and the viscosity value of each experimental group is 5% higher than that of the corresponding control group on average. The nitrate reducing bacterium is applied in the field of oilfield development.

Description

technical field [0001] The invention relates to a strain of nitrate reducing bacteria and its application. Background technique [0002] At present, polymer is the main flooding medium for tertiary oil recovery in oilfields. In 2011, the oil production of polymer flooding in Daqing Oilfield exceeded 13 million tons, more than one-third of the total output of the oilfield. Therefore, ensuring the production effect of polymer flooding is of great significance to the high and stable production of oilfields. With the continuous deepening of research on polymer flooding technology and the continuous expansion of the application scale of polymer flooding field, problems such as large viscosity loss and low viscosity retention rate during polymer injection are gradually exposed on site. These problems cause a certain gap between the viscosity of the polymer injected into the formation and the design requirements, reducing the flooding efficiency, which brings economic losses to th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C09K8/582C12R1/01
Inventor 盖忠辉皮文清蒋立民
Owner 黑龙江吉纳森生物工程股份有限公司
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