Serratia marcescens M7a and application of Serratia marcescens M7a in heavy metal ion removing
A technology of Serratia marcescens and heavy metal ions, applied in the direction of bacteria, microorganism-based methods, and restoration of contaminated soil, etc., can solve the conditions of small heavy metal-resistant populations, large impact, strain screening, and strain-specific growth conditions Less research on issues such as
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[0112] Remove heavy metal ions Mn 2+ strain screening
[0113] 1 Experimental materials
[0114] 1.1 Experimental strains
[0115] Sample source
[0116] Soil or water samples from different locations were collected from Weifang, Rizhao, Nanjing and other areas. The soil samples were collected from 1-4cm topsoil in multiple places in the sampling area and mixed separately. There are 27 samples in total.
[0117] 1.2 Experimental Instruments
[0118] Vertical pressure steam sterilizer (Hirayama HVE-50, Japan); air bath shaker (IKA KS 4000ic, Germany); analytical balance (Mettler Toledo PL 602-S, Switzerland); constant temperature incubator (MMM Incucell 111, Germany); gun (Eppendorf, Germany); desktop high-speed centrifuge (Eppendorf 5417R, Germany); inductively coupled plasma mass spectrometry (ICP-MS, Agilent 7700X, USA), etc.
[0119] 1.3 Experimental reagents
[0120] Manganese chloride [MnCl 2 ×4H 2 O] Chemical reagents were of analytical grade. Calculate the mas...
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