A preparation method of phosphorylated krill peptide capable of preventing and treating osteoporosis

Abstract

The invention provides a phosphorylated krill peptide. A preparation method comprises the following steps: (1) degreasing krill powder, then adding a NaOH solution into the krill powder and centrifuging to obtain a supernatant, regulating a pH value of the supernatant until reaching the isoelectric points of protein and centrifuging to obtain a precipitate; (2) preparing the krill protein precipitate into an aqueous solution, adding a phosphate, regulating the pH value of the solution and performing spray drying on the solution so as to obtain krill protein powder; standing the krill protein powder at a high temperature so as to obtain phosphorylated krill protein; (3) repeatedly washing the phosphorylated krill protein with distilled water and centrifuging; and (4) preparing the phosphorylated krill protein into the aqueous solution, adding 2M NaOH, regulating the pH value of the solution, adding a protease into the solution, hydrolyzing for 3 to 6 hours at a proper temperature, heating to 100 DEG C and stopping an enzyme reaction, centrifuging to obtain the supernatant and performing the spray drying on the supernatant so as to obtain the phosphorylated krill peptide. The animal experiment proves that the prepared phosphorylated krill peptide can be used for obviously regulating the bone metabolism level of a model rat with the osteoporosis so as to enhance the bone mineral density and improve the bone biomechanical property, thereby having obvious functions for preventing and treating the osteoporosis.

Description

technical field [0001] The invention relates to a preparation method of phosphorylated krill peptide with the effect of preventing and treating osteoporosis and its application in the fields of food, functional food and medicine. Background technique [0002] Osteoporosis, a disease of the skeletal system that increases the risk of fracture due to decreased bone strength, has become an increasingly serious social health problem worldwide. It is estimated that the number of patients in the world has exceeded 200 million at present, of which the incidence rate of 60-70 years old is 58%, and the incidence rate of over 70 years old is almost 100%, especially the incidence rate of postmenopausal women is higher. Currently, the drugs on the market for the prevention and treatment of osteoporosis mainly include estrogen receptor modulators, bisphosphonates, calcitonin, fluoride preparations, vitamin D and its active metabolites, etc. However, long-term use of these drugs has stron...

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Problems solved by technology

Cai Weiwang et al. have reported that low doses of Antarctic krill powder can improve the symptoms of osteoporosis model rats, in which fluorine plays a major role (Chinese Journal of Biological Products, 2013, 5, 643-647); Gigliotti, et al. reported Antarctic Krill crude protein can effectively alleviate the symptoms of bone calcium deposition and bone density loss in early kidney injury model rats, and has no significant effect on bone mass and bone strength, but no functional components in the test substance have been reported (UrologicalResearch, 2011, 39,59-67)

At present, there is no relevant report on the prevention and treatment of osteoporosis by phosphorylated krill peptide

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