A preparation method of phosphorylated krill peptide capable of preventing and treating osteoporosis
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A technology of phosphorylated krill and krill, applied in bone diseases, hydrolyzed protein components, food science, etc., can solve the problems of unreported functional ingredients, achieve no environmental pollution, high product safety, and prevent and treat osteoporosis Effect
Active Publication Date: 2016-03-23
OCEAN UNIV OF CHINA
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Cai Weiwang et al. have reported that low doses of Antarctic krill powder can improve the symptoms of osteoporosis model rats, in which fluorine plays a major role (Chinese Journal of Biological Products, 2013, 5, 643-647); Gigliotti, et al. reported Antarctic Krill crude protein can effectively alleviate the symptoms of bone calcium deposition and bone density loss in early kidney injury model rats, and has no significant effect on bone mass and bone strength, but no functional components in the test substance have been reported (UrologicalResearch, 2011, 39,59-67)
At present, there is no relevant report on the prevention and treatment of osteoporosis by phosphorylated krill peptide
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Embodiment 1
[0014] (1) Take 10 kg of Antarctic krill powder, add 100 L of 95% ethanol, stir in the reaction kettle for 6 hours, centrifuge to collect the precipitate, and dry it in vacuum to obtain defatted krill powder;
[0015] (2) Add 80L NaOH solution to defatted krill powder, stir and extract at 60°C for 40min, centrifuge to obtain supernatant A;
[0016] (3) Add 6M HCl to the supernatant A, adjust the pH value of the supernatant A to the isoelectric point, let it stand for 20 minutes, and centrifuge to obtain the precipitate B. Add 5 times the volume of water and 0.2M potassium pyrophosphate to the solution, and add to the solution Add 2M HCl to the solution, adjust the pH value of the solution to 2.5, and spray dry to obtain krill protein powder;
[0017] (4) Phosphorylated krill protein powder was obtained by heating krill protein powder at 80°C for 48 hours to obtain phosphorylated krill protein powder;
[0018] (5) Add 5 times the volume of water to the phosphorylated protein p...
Embodiment 2
[0021] (1) Take 10kg of Antarctic krill powder, add 80L of 95% ethanol, stir in the reaction kettle for 7 hours, separate the precipitate, and dry it in vacuum to obtain defatted krill powder;
[0022] (2) Add 70L NaOH solution to defatted krill powder, stir and extract at 50°C for 60 minutes, centrifuge to obtain supernatant A;
[0023] (3) Add 4M HCl to the supernatant A, adjust the pH value of the supernatant A to the isoelectric point, let it stand for 30 minutes, and centrifuge to obtain the precipitate B. Add 6 times the volume of water and 0.15M sodium metaphosphate to the solution. Add 2M HCl to the solution, adjust the pH value of the solution to 3.5, and spray dry to obtain krill protein powder;
[0024] (4) Krill protein powder was heated at 85°C for 24 hours for phosphorylation and derivatization to obtain phosphorylated krill protein powder;
[0025] (5) Add 6 times the volume of water to the phosphorylated krill protein powder, mix well, and centrifuge to obtain...
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Abstract
The invention provides a phosphorylated krill peptide. A preparation method comprises the following steps: (1) degreasing krill powder, then adding a NaOH solution into the krill powder and centrifuging to obtain a supernatant, regulating a pH value of the supernatant until reaching the isoelectric points of protein and centrifuging to obtain a precipitate; (2) preparing the krill protein precipitate into an aqueous solution, adding a phosphate, regulating the pH value of the solution and performing spray drying on the solution so as to obtain krill protein powder; standing the krill protein powder at a high temperature so as to obtain phosphorylated krill protein; (3) repeatedly washing the phosphorylated krill protein with distilled water and centrifuging; and (4) preparing the phosphorylated krill protein into the aqueous solution, adding 2M NaOH, regulating the pH value of the solution, adding a protease into the solution, hydrolyzing for 3 to 6 hours at a proper temperature, heating to 100 DEG C and stopping an enzyme reaction, centrifuging to obtain the supernatant and performing the spray drying on the supernatant so as to obtain the phosphorylated krill peptide. The animal experiment proves that the prepared phosphorylated krill peptide can be used for obviously regulating the bone metabolism level of a model rat with the osteoporosis so as to enhance the bone mineral density and improve the bone biomechanical property, thereby having obvious functions for preventing and treating the osteoporosis.
Description
technical field [0001] The invention relates to a preparation method of phosphorylated krill peptide with the effect of preventing and treating osteoporosis and its application in the fields of food, functional food and medicine. Background technique [0002] Osteoporosis, a disease of the skeletal system that increases the risk of fracture due to decreased bone strength, has become an increasingly serious social health problem worldwide. It is estimated that the number of patients in the world has exceeded 200 million at present, of which the incidence rate of 60-70 years old is 58%, and the incidence rate of over 70 years old is almost 100%, especially the incidence rate of postmenopausal women is higher. Currently, the drugs on the market for the prevention and treatment of osteoporosis mainly include estrogen receptor modulators, bisphosphonates, calcitonin, fluoride preparations, vitamin D and its active metabolites, etc. However, long-term use of these drugs has stron...
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