Preparation method of notoginseng leaf total saponins

A technology of Panax notoginseng leaves and total saponins, which is applied in the field of preparation of Panax notoginseng leaves total saponins, can solve the problems of short service life of resin, serious resin pollution, etc., and achieves the effects of high yield and treatment of insomnia.

Inactive Publication Date: 2014-07-30
YUNNAN WEIHE PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the products obtained from the reported Notoginseng saponin extraction process did not emphasize the index component Rb in the leaf glycosides. 3 content, no targeted enrichment of Rb 3 , the total content of total saponins is mostly used as the basis for quality, and the stability and quality reliability of the product cannot be guaranteed
At the same time, when the resin is used for purification, the pollution of the sample to the resin is more serious, and the service life of the resin is shorter

Method used

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  • Preparation method of notoginseng leaf total saponins

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The preparation method of Panax notoginseng total saponins comprises the steps:

[0031] Step (1): Grind 30kg of Panax notoginseng leaves, add 70% ethanol whose mass is 4 times that of Panax notoginseng leaves, conduct continuous ultrasonic countercurrent extraction for 60 minutes, collect the extract, filter, concentrate to a clear paste, and the relative density of the clear paste is 1.2 (60 ℃); wherein, the leaf-stem mass ratio of the notoginseng is 3 / 7;

[0032] Step (2): Add water to the clear paste obtained in step (1) to make the density 1.08 (60°C), heat to 75°C, add 750 ml of gelatin, stir until evenly mixed, then heat up to 82°C, add 375 ml chitosan, stirred until evenly mixed, lowered to room temperature and stood still overnight, centrifuged to obtain a clear solution;

[0033] Concentrate the clear solution to a density of 1.08 (60°C), add ethanol to make the ethanol content 30%, stir until the mixture is uniform, stand at room temperature overnight, and c...

Embodiment 2

[0036] The preparation method of Panax notoginseng total saponins comprises the steps:

[0037] Step (1): Grind 15kg of Panax notoginseng leaves, add 70% ethanol whose mass is 5 times that of Panax notoginseng leaves, and conduct continuous ultrasonic countercurrent extraction for 60 minutes, collect the extract, filter, concentrate to a clear paste, and the relative density of the clear paste is 1.2 (60 ℃); wherein, the leaf-stem mass ratio of Panax notoginseng is 1 / 2;

[0038] Step (2): Add water to the clear paste obtained in step (1) to make the density 1.10 (60°C), heat to 78°C, add 375 ml ZTC 1+1, stir until well mixed, then heat up to 85°C At this time, add 187.5 ml of gelatin, stir until the mixture is uniform, lower to room temperature and stand overnight, and centrifuge to obtain a clear solution;

[0039] Concentrate the clear solution to a density of 1.08 (60°C), add ethanol to make the ethanol content 40%, stir until the mixture is uniform, stand at room temperat...

Embodiment 3

[0042] The preparation method of Panax notoginseng total saponins comprises the steps:

[0043] Step (1): Grind 20kg of Panax notoginseng leaves, add 80% ethanol whose mass is 4.5 times that of Panax notoginseng leaves, and conduct continuous ultrasonic countercurrent extraction for 60 minutes, collect the extract, filter, concentrate to a clear paste, and the relative density of the clear paste is 1.2 (60 ℃); wherein, the mass ratio of the leaf and stem of the notoginseng is 2 / 3;

[0044] Step (2): Add water to the clear paste obtained in step (1) to make the density 1.08 (60°C), heat to 78°C, add 500 ml ZTC 1+1, stir until well mixed, and then heat up to 85°C , add 250ml chitosan, stir until mixed evenly, drop to room temperature and stand overnight, centrifuge to obtain a clear solution;

[0045] Concentrate the clear solution to a density of 1.08 (60°C), add ethanol to make the ethanol content 50%, stir until the mixture is uniform, stand at room temperature overnight, an...

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Abstract

The invention relates to a preparation method of notoginseng leaf total saponins, and belongs to the technical field of natural medicine extraction. The preparation method comprises the following steps: adding a solvent to notoginseng leaves, carrying out ultrasonic wave countercurrent continuous extraction, filtering, collecting filtrate, concentrating to obtain clear paste, adding a natural clarifying agent for clarification, centrifuging, adding ethyl alcohol to a clear solution to ensure that the ethyl alcohol content of the clear solution is 30-50%, and carrying out alcohol precipitation; and purifying the clear solution subjected to alcohol precipitation by using a desugaring resin column, eluting by using ethyl alcohols with different concentrations, decolorizing by using anion exchange resin after desugaring, finally concentrating, and carrying out microwave drying so as to obtain the notoginseng leaf total saponins. The preparation method has the advantages that the obtained notoginseng leaf total saponins are off-white, the texture of the powder is loose, the yield of total glycosides is above 8%, the content of the total glycosides is not lower than 90% (UV-ultraviolet), the obtained notoginseng leaf total saponins mainly comprise Rb3, Rc, Fc, Fa and the like, and the content of the Rb3 is greater than or equal to 40% (HPLC- High Performance Liquid Chromatography); the process is simple, the realization of industrialization is facilitated, and the product quality is substantially improved.

Description

technical field [0001] The invention belongs to the technical field of natural medicine extraction, and in particular relates to a preparation method of total saponins of notoginseng leaves. Background technique [0002] Panax notoginseng ( Panax notoginseng (Burk.) F.H.Chen) is a well-known traditional Chinese medicine, and its roots are usually used as medicine. Notoginseng leaves are warm in nature and pungent in taste, and are often drunk as tea among the people. Fresh tender leaves can be eaten as vegetables. "Compendium of Materia Medica" states that Panax notoginseng "cures wounds, falls, and bleeding, stops immediately after application, and bruises dissipate overnight, and the rest of the work is the same as the root." Modern research shows that Notoginseng leaf has the functions of hemostasis, detumescence, and pain relief. [0003] Since the 1980s, systematic research has been carried out on the chemical components of Notoginseng leaves, and it has been found ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/258A61K127/00
Inventor 郑立雄陈金东任杨帆邵艳红
Owner YUNNAN WEIHE PHARMA
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