A kind of method for quantitative analysis and detection of auroside
A technique of aureoside and a detection method, which is applied in the directions of analyzing materials, measuring devices, material separation, etc., can solve the problems of difficulty in complete separation, inability to qualitatively and quantitatively analyze the aureoside, and achieves good repeatability and accuracy. high effect
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Embodiment 1
[0024] Weigh 15g of the fresh wild Auranthus clematis plant after drying, pulverize it, ultrasonically extract it with methanol at 40° for three times, and concentrate the extract in vacuum to obtain an extract. The extract was placed in a Soxhlet extractor, extracted with petroleum ether, diethyl ether and chloroform under reflux for 2 hours respectively, the residue was dissolved in 100 mL of water and filtered, and 1 mL of the filtrate was added to dilute to 25 mL with water. Take 1 mL of the solution at a constant volume and filter it through a 0.22 μm microporous membrane as the auroglutin sample solution for HPLC-ESI / MS.
[0025] Take the pure product of auroside to prepare a standard solution of auroside with a concentration of 10mg / mL. Take 1mL of the aureoside standard solution in constant volume and filter it through a 0.22 μm microporous membrane as the standard solution of HPLC-ESI / MS.
[0026] Analyzing the concentration of auroglutin in the above-mentioned aurog...
Embodiment 2
[0034] After the fresh clematis plant is dried, weigh 15g and pulverize it, process and measure and calculate the results with the same steps and methods as in Example 1, wherein A1 is as figure 1 As shown in a, A2 is shown as figure 1 As shown in c,:
[0035] The auroglutinin content (mg / mL)=10mg / mL×A2 / A1 in the auroglutinin test sample liquid
[0036] Auroglutin content (mg / g) in the plant sample=Auroglutin content (mg / mL) in the sample liquid to be tested for auroglutin × 2500mL / 15g
[0037] The above-mentioned pretreatment of the crude extract, preparation of the sample solution, and determination of the content of the sample solution and the standard sample were repeated three times, and the results were averaged and the average deviation was calculated.
[0038] Result: Table 2 lists the peak time and peak area of the EIC spectrum of the auroglutin in A. chinensis determined experimentally. It is calculated that the content of auroglucoside in the wild clematis is 2...
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