Method for production of bacillus subtilis preparation by utilizing ethanol industrial yellow water
A technology of Bacillus subtilis and alcohol industrial yellow water, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of increasing costs, affecting the application of Bacillus subtilis, and high production costs, so as to increase production, The effect of improving comprehensive utilization value and reducing production cost
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[0030] Example 1: A method for producing Bacillus subtilis preparations using alcohol industrial yellow water. This method uses corn as a raw material to produce alcohol yellow water. The specific steps are as follows:
[0031] (1) Collect alcohol industrial yellow water directly from the plate frame of the plate and frame filter press, quickly put it into a sealed container, and then take a sample to determine the content of nutrients such as protein, cellulose, amino acids, organic acids, mineral elements, etc. The content of various nutrients in the yellow water of the alcohol industry, the measurement results are shown in the attachment figure 1 , And according to the actual measurement value and the nutritional needs of Bacillus subtilis growth and reproduction, nutritional supplements are carried out. The specific types and quantities of supplements are: 5g of urea, 2g of glucose, 2g of sodium chloride and 2g of sodium chloride per liter of the above-mentioned alcoholic indus...
Example Embodiment
[0041] Example 2: A method for producing a Bacillus subtilis preparation using alcohol industrial yellow water. This method uses corn as a raw material to produce alcohol yellow water. The specific steps are as follows:
[0042] (1) Collect alcohol industrial yellow water directly from the frame of the plate and frame filter press, quickly put it into a sealed container, and then take a sample to determine the content of nutrients such as protein, cellulose, amino acids, organic acids, mineral elements, etc. The content of various nutrients in the yellow water of the alcohol industry, the measurement results are shown in the attachment figure 1 , And according to the actual measured value and the nutritional needs of Bacillus subtilis growth and reproduction, nutritional supplements are carried out. The specific types and quantities of nutritional supplements are: 5g of urea, 2g of glucose, 2g of sodium chloride and 3g corn flour;
[0043] (2) Sterilize the alcoholic industrial yel...
Example Embodiment
[0052] Example 3: A method for producing a Bacillus subtilis preparation using alcohol industrial yellow water. This method uses corn as a raw material to produce alcohol yellow water. The specific steps are as follows:
[0053] (1) Collect alcohol industrial yellow water directly from the frame of the plate and frame filter press, quickly put it into a sealed container, and then take a sample to determine the content of nutrients such as protein, cellulose, amino acids, organic acids, mineral elements, etc. The content of various nutrients in the yellow water of the alcohol industry, the measurement results are shown in the attachment figure 1 , And according to the actual measured value and the nutritional needs of Bacillus subtilis growth and reproduction, nutritional supplements are carried out. The specific types and quantities of nutritional supplements are: 5g of urea, 2g of glucose, 2g of sodium chloride and 3g corn flour;
[0054] (2) Sterilize the alcoholic industrial yel...
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