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Bioenzyme-method extraction process for extracting pectin from papaya peel

A biological enzyme method and extraction technology, applied in the field of food deep processing, can solve the problems of papaya pectin that has not been reported, and achieve the effects of improving comprehensive utilization value, reducing environmental pollution, and increasing farmers' income

Pending Publication Date: 2021-11-30
NANNING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] However, there is no report on the research on extracting papaya pectin by compound enzyme method at present.

Method used

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  • Bioenzyme-method extraction process for extracting pectin from papaya peel
  • Bioenzyme-method extraction process for extracting pectin from papaya peel
  • Bioenzyme-method extraction process for extracting pectin from papaya peel

Examples

Experimental program
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Effect test

Embodiment 1

[0095]A bio-enzymatic extraction process of pectin in papaya peel, adding papaya peel powder into buffer solution, preheating in a constant temperature water bath, adding compound enzyme, stirring for enzymatic hydrolysis, centrifuging, adding ethanol to the supernatant and standing still for alcohol analysis , and then through vacuum filtration and drying, to obtain the pectin; specifically comprising the following steps:

[0096] (1) Preparation of papaya peel powder: select the papaya peel that has just been peeled off, remove pulp, impurities, mildew, and rotten papaya peel, decoct in boiling water to passivate, then rinse and cool with distilled water, then soak in distilled water, repeat Wash and remove part of the soluble sugar on the papaya skin, remove it and put it on a bamboo basket to drain the water, chop it and put it in an electric constant temperature blast drying oven to dry, crush it, sieve it, and dry it for later use;

[0097] The passivation temperature is...

Embodiment 2

[0102] A bio-enzymatic extraction process of pectin in papaya peel, adding papaya peel powder into buffer solution, preheating in a constant temperature water bath, adding compound enzyme, stirring for enzymatic hydrolysis, centrifuging, adding ethanol to the supernatant and standing still for alcohol analysis , and then through vacuum filtration and drying, to obtain the pectin; specifically comprising the following steps:

[0103] (1) Preparation of papaya peel powder: select the papaya peel that has just been peeled off, remove pulp, impurities, mildew, and rotten papaya peel, decoct in boiling water to passivate, then rinse and cool with distilled water, then soak in distilled water, repeat Wash and remove part of the soluble sugar on the papaya skin, remove it and put it on a bamboo basket to drain the water, chop it and put it in an electric constant temperature blast drying oven to dry, crush it, sieve it, and dry it for later use;

[0104] The passivation temperature i...

Embodiment 3

[0109] A bio-enzymatic extraction process of pectin in papaya peel, adding papaya peel powder into buffer solution, preheating in a constant temperature water bath, adding compound enzyme, stirring for enzymatic hydrolysis, centrifuging, adding ethanol to the supernatant and standing still for alcohol analysis , and then through vacuum filtration and drying, to obtain the pectin; specifically comprising the following steps:

[0110] (1) Preparation of papaya peel powder: select the papaya peel that has just been peeled off, remove pulp, impurities, mildew, and rotten papaya peel, decoct in boiling water to passivate, then rinse and cool with distilled water, then soak in distilled water, repeat Wash and remove part of the soluble sugar on the papaya skin, remove it and put it on a bamboo basket to drain the water, chop it and put it in an electric constant temperature blast drying oven to dry, crush it, sieve it, and dry it for later use;

[0111] The passivation temperature i...

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Abstract

The invention discloses a bioenzyme-method extraction process for extracting pectin from papaya peel. The process comprises the following steps: adding papaya peel powder into a buffer solution, carrying out preheating in a constant-temperature water bath pot, then, adding a compound enzyme, carrying out stirring for enzymolysis, carrying out centrifuging, adding ethanol into supernatant, carrying out standing for alcohol precipitation, and carrying out vacuum filtration and drying, thereby obtaining the pectin. According to the process, the pectin in the papaya peel is extracted by utilizing single compound enzyme (formed by mixing xylanase and cellulase) extraction and adopting an efficient, green and environment-friendly ethanol precipitation method for the first time, original pectinase contained in papayas is passivated by boiling, and the influence on an experimental result due to self enzymolysis is prevented, so that the comprehensive utilization value of the papayas is increased, the income of papaya planting farmers is increased, resource waste is avoided, and environmental pollution is reduced.

Description

technical field [0001] The invention belongs to the technical field of food deep processing, and in particular relates to a biological enzymatic extraction process of pectin in papaya peel. Background technique [0002] Pectin is a soluble heteropolysaccharide, and its molecular structure is mainly composed of D-(+)-galacturonic acid connected by α-1,4 glycosidic bonds to form a main chain, rhamnose, galactose, xylose, trehalose, acetic acid and Methanol and the like are connected to the main chain as side chains. Pectin widely exists in the middle layer of plant cells and in primary and secondary cell walls. It promotes the formation of plant cell morphology and the improvement of mechanical strength through cross-linking with cellulose, hemifiber, lignin and extensin. [0003] In addition to its good gel properties, pectin also has biological functions such as promoting human gastrointestinal motility, absorbing heavy metals, and removing various biological toxins, so it ...

Claims

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Application Information

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IPC IPC(8): A23L29/231A23L33/125C08B37/06
CPCA23L29/231A23L33/125C08B37/0045A23V2002/00A23V2200/228A23V2200/30A23V2250/5072
Inventor 程忠李敏敏杨媚媚韦云伊许文婷
Owner NANNING UNIV
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