A kind of preparation method of insect sample for scanning electron microscope

A scanning electron microscope and insect technology, applied in the field of insect sample preparation, can solve the problems of slow penetration of glutaraldehyde, high degree of image shrinkage, sample deformation, etc., to achieve great practical application value, good insect morphological structure, prevent shrunken effect
CN104020030BInactive Publication Date: 2017-03-01BEIJING UNIV OF AGRI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
BEIJING UNIV OF AGRI
Publication Date
2017-03-01
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides a preparation method of an insect sample for a scanning electron microscope. The preparation method comprises the following steps of: cleaning insect material, fixing tissues, dewatering, replacing, drying and spraying metal and the like, wherein the tissue fixing comprises the steps of: immersing the insect material in stationary liquid, heating in a microwave oven for a period of time, and respectively fixing for a period of time under room temperature and low temperature. The method provided by the invention is rapid and simple and easy to carry out, a clear scanning electron microscope image of a surface structure of an insect can be obtained, the whole morphological structure of the insect can be better maintained, and the deformation, the shrinkage and the collapse are hardly caused. The preparation method has high practical application value.
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Description

technical field

[0001] The invention relates to a method for preparing an insect sample for a scanning electron microscope. Background technique

[0002] Insects are an important part of the animal kingdom. Scanning electron microscopes are widely used in the study of insect structures: scanning electron microscopes are widely used in insect physiology, insect anatomy, and insect systematics. A large number of relevant literature and practical operations show that the preparation of insect samples directly affects the objectivity and authenticity of the observation site.

[0003] Insect samples are generally prepared through steps such as sample cleaning, tissue fixation, dehydration, replacement, drying, and gold spraying. Because the overall insect material is large, and the outer skin of the insect has a certain degree of ossification, it is not easy for the fixative to penetrate the internal organs and tissues of the insect. Whether the ultrastructure of each part of t...

Claims

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