Method for inhibiting contamination and browning of toxicodendron vernicifluum explants

An explant and browning technology, which is applied in botany equipment and methods, horticultural methods, plant regeneration, etc., can solve the problems of lacquer tree explant pollution and browning

Active Publication Date: 2014-09-10
SOUTHWEST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] What the present invention aims to solve is the pollution and browning of sumac explants in the high-efficiency sumac tissue culture and rapid propagation technology system, thereby proposing a method that can effectively remove surface bacteria and endophytic fungi and prevent explants from browning method

Method used

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  • Method for inhibiting contamination and browning of toxicodendron vernicifluum explants
  • Method for inhibiting contamination and browning of toxicodendron vernicifluum explants

Examples

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Embodiment 1

[0021] (1) Take 1-2 year old stems or young leaves of lacquer trees that are robust and free from diseases and insect pests, wipe them lightly with a brush dipped in soapy water to clean the dirt on the surface of the materials, put them in a beaker, and rinse them with tap water for 2 hours;

[0022] (2) Transfer to the ultra-clean workbench, soak in 75% ethanol for 20 s, and rinse with sterile water for 3 min;

[0023] (3) Soak in 1% benzalkonium chloride for 1 min, rinse with sterile water for 3 min;

[0024] (4) Disinfect with 0.1% mercury liter for 20 minutes, rinse with sterile water 5 to 8 times, 2 minutes each time;

[0025] (5) Dry the water with sterilized filter paper, and cut the material into 1 cm stem segments or 1 cm × 1 cm cubes;

[0026] (6) Inoculate in White medium supplemented with cysteine, the ratio is 1 L of White medium supplemented with 100 mg cysteine, 30 g sucrose, 6 g agar powder, and the pH is adjusted to 5.8;

[0027] (7) Culture in dark for 2 w...

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Abstract

The invention relates to the plant tissue culture technical field, and especially relates to a method for inhibiting contamination and browning of explants in tissue culture. The invention discloses the method for inhibiting contamination and browning of the toxicodendron vernicifluum explants, and is characterized in that the method comprises a step of adopting benzalkonium chloride to disinfect and treat the toxicodendron vernicifluum explants and a step of inoculating a cysteine-added White culture medium with the disinfected material and carrying out dark culture. The method is used for inhibiting contamination of the toxicodendron vernicifluum explants and preventing browning of the toxicodendron vernicifluum explants, provides sufficient sterile explants for toxicodendron vernicifluum tissue culture, powerfully ensures the success rate of subsequent tissue culture, and moreover, is low in cost and suitable for industrialized production.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for inhibiting contamination and browning of explants in tissue culture. Background technique [0002] sumac ( Toxicodendron vernicifluum ) is a plant of the genus Anacardiacceae (Anacardiacceae). It is named for the production of lacquer. It is a unique economic tree with the most ethnic characteristics in my country. Raw lacquer is currently the only green plant in the world. Under the catalysis of biological enzymes, normal temperature Biopolymer coatings that can be cured to form films have been widely used in military industry, chemical industry, light industry, petroleum, mining, art, etc., and are also important export agricultural products in my country. Because lacquer has multiple uses such as natural paint, oil and wood, it is not only an important special-purpose economic tree species in my country, but also an excellent ecological tree species. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 何承忠李旦赵月明段安安祝建兵廖声熙许玉兰
Owner SOUTHWEST FORESTRY UNIVERSITY
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