Flammulina velutipes polysaccharide and extracting and purifying method thereof as well as application of flammulina velutipes polysaccharide as tobacco humectant

A technology of Flammulina velutipes polysaccharide and purification method is applied in the application field of extraction and purification, Flammulina velutipes polysaccharide, and tobacco moisturizing agent, and achieves the effects of reducing impurities, obvious moisturizing effect, and broad application prospect.

Inactive Publication Date: 2014-09-17
CHINA TOBACCO HENAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no relevant report on the application of Flammulina velutipes polysaccharide in cigarettes as a tobacco humectant at home and abroad

Method used

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  • Flammulina velutipes polysaccharide and extracting and purifying method thereof as well as application of flammulina velutipes polysaccharide as tobacco humectant
  • Flammulina velutipes polysaccharide and extracting and purifying method thereof as well as application of flammulina velutipes polysaccharide as tobacco humectant

Examples

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Effect test

Embodiment 1

[0017] A method for extracting and purifying polysaccharides from Flammulina velutipes, comprising the following steps: extract 300 g of dried Flammulina velutipes with 1800 ml of ether Soxhlet for 2 hours to remove oil, and evaporate the residue to dryness. The residue after evaporating to dryness was extracted three times with 750ml boiling water, combined the boiling water extracts obtained three times, concentrated, centrifuged, and the supernatant was taken, and the supernatant was added with absolute ethanol 3 times the volume of the clear liquid for alcohol precipitation, and stood overnight (12h), centrifuge again to collect the precipitate. The precipitate was washed three times with 300ml ethanol and 300ml acetone in turn, and then dissolved in distilled water, then papain with a weight of 1‰ of the precipitate was added and placed in a water bath at 50°C for 2 hours, and the protein was removed by the Sevage method. Add 8g of activated carbon and place it in a 70°C ...

Embodiment 2

[0020] A method for extracting and purifying polysaccharides from Flammulina velutipes, comprising the steps of: extracting 300 g of dried Flammulina velutipes with 2000 ml of ether Soxhlet for 4 hours to remove oil, and evaporating the residue to dryness. The residue after evaporating to dryness was extracted with 750ml of boiling water for 3 times, and the boiling water extracts obtained three times were combined, concentrated, centrifuged, and the supernatant was taken, and anhydrous ethanol with 3 times the volume of the clear liquid was added for alcohol precipitation, and stood for 12 hours , and centrifuge again to collect the precipitate. The precipitate was washed three times with 300ml of ethanol and 300ml of acetone in turn, then dissolved in distilled water, and then papain with a weight of 2‰ of the precipitate was added, placed in a water bath at 50°C for 4 hours, and the protein was removed by Sevage method. Add 8g of activated carbon and place it in a 70°C wate...

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Abstract

The invention relates to a flammulina velutipes polysaccharide and an extracting and purifying method thereof. The extracting and purifying method particularly comprises the following steps of: carrying out Soxhlet extraction on crushed dry flammulina velutipes by using diethyl ether, then spin-drying residues, and then extracting by using boiling water; concentrating and centrifugalizing a boiling water extracting solution; carrying out ethanol precipitation standing on a supernatant, and collecting precipitates by centrifugalizing again; washing, and then dissolving by using distilled water; then adding 0.001-0.002% of papain to a 50+ / -5 DEG C water bath, standing for 2-4 hours, and removing the protein by adopting a Sevage method; carrying out reduced pressure concentration after active carbon is decolored; cooling, and then carrying out running water dialysis; carrying out the ethanol precipitation standing again after the dialysis is finished, and drying the precipitates at 60-70 DEG C to obtain crude polysaccharide. A test proves that the flammulina velutipes polysaccharide disclosed by the invention plays a role of very good physical and sensory humidity preservation, can reduce the pungency and offensive odor of a cigarette and enhance the mellowness and comfortability of cigarette smoke and can be applied in the cigarette as a tobacco humidity preserving agent with excellent property.

Description

technical field [0001] The invention belongs to the technical field of humectants, and in particular relates to polysaccharides from Flammulina velutipes, an extraction and purification method and its application as a humectant for tobacco. Background technique [0002] At present, glycerin, propylene glycol, sorbitol and other polyhydroxy substances are mainly used as humectants in the cigarette industry in my country. The main purpose of using such humectants is to maintain the moisture content of shredded tobacco during processing and improve the processing resistance of shredded tobacco. The above-mentioned humectant has unsatisfactory effects on maintaining the moisture content of finished cigarettes and improving smoking comfort. [0003] Polysaccharides are composed of a large number of monosaccharides, and a large number of hydroxyl groups form hydrogen bonds with water molecules, which can make them have strong water holding capacity. At present, there is no relevan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A24B15/30
Inventor 陈芝飞刘强芦昶彤赵永振周浩郝辉孙志涛
Owner CHINA TOBACCO HENAN IND
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