Cartridge For Nucleic Acid Amplification Reacion
A nucleic acid amplification reaction, nucleic acid technology, applied in bioreactor/fermenter combination, bioreactor/fermenter for specific purposes, laboratory containers, etc., can solve the problems that have not been developed, and achieve simple nucleic acid Effects of Amplification Reactions
Inactive Publication Date: 2014-09-17
SEIKO EPSON CORP
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- Abstract
- Description
- Claims
- Application Information
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Problems solved by technology
[0003] However, recently, in addition to conventional PCR devices, thermal cyclers that can easily control the heating time have also been developed (see Patent Document 1), but cartridges that can be applied to these devices have not yet been developed.
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[0223] (1) Composition of nucleic acid extraction equipment
[0224] Fig. 16 shows an exploded view of the nucleic acid extraction instrument used in this example before assembly ( Figure 16A ) And the completed drawing after assembly ( Figure 16B ).
[0225] The device has a capillary 200 and a tank 120 for injecting liquid into the capillary 200. The capillary 200 and the tank 120 can constitute a kit 130 for assembling a nucleic acid extraction device.
[0226] The capillary 200 has a stopper 110, and is provided with first to seventh stoppers inside, and is respectively filled with oil 710, cleaning solution 720, oil 730, reverse transcription reaction solution 740, oil 750, eluent 760, and oil 770 in sequence. Oil has the function of separating each aqueous solution.
[0227] The tank 120 has an opening 121, a cover 122 detachable from the opening 121, a space 123, and a solution 124. In addition, the dissolving solution 124 contains magnetic beads 125 of the silica coated o...
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Abstract
A cartridge for nucleic acid amplification reaction includes a tube that has a first plug, a second plug formed of a second washing solution washes nucleic acid-binding solid-phase carriers having bound to a nucleic acid, a third plug, a fourth plug formed of a reverse transcription reaction solution which undergoes phase separation when being mixed with oil and in which a reverse transcription reaction occurs, a fifth plug formed of a third oil, a sixth plug formed of an eluate which causes the nucleic acid to be eluted from the nucleic acid-binding solid-phase carriers having bound to the nucleic acid, and a seventh plug in this order in the inside of the tube; a container for nucleic acid amplification reaction that is in communication with the tube and contains oil; and a plunger that pushes liquid to the container for nucleic acid amplification reaction out of the tube.
Description
Technical field [0001] The present invention relates to a Cartridge for nucleic acid amplification reaction. Background technique [0002] Boom et al. reported a method for extracting nucleic acids from biological materials more easily using a nucleic acid-binding solid phase carrier such as silica particles and a chaotropic agent (see Non-Patent Document 1). Including the method of Boom et al., the method of extracting nucleic acid by using a nucleic acid-binding solid phase carrier such as silica and a chaotropic agent to adsorb nucleic acid on the carrier mainly consists of the following three steps: (1) In the presence of a chaotropic agent, The step of adsorbing nucleic acid to the nucleic acid-binding solid phase carrier (adsorption step), (2) the step of washing the nucleic acid-adsorbed carrier with a cleaning solution (cleaning step) in order to remove non-specifically bound inclusions and chaotropic agents, and (3) A step of eluting nucleic acid from the carrier with w...
Claims
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IPC IPC(8): C12M1/00
CPCB01L7/52B01L7/525B01L2200/0673B01L3/502761B01L3/502784B01L2200/0647B01L2300/0838B01L2400/043B01L2400/0478G01N35/0098G01N2035/00366G01N2035/0436B01L3/502
Inventor 齐藤祐司高城富美男
Owner SEIKO EPSON CORP