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A method for directly regenerating plants from tung leaf petioles

A technology for regenerating plants and petioles, which is applied in the field of direct regeneration of tung oil tung petioles, can solve the problems of rareness and short cultivation period, and achieve the effect of strong growth, normal growth of buds and obvious main stems

Active Publication Date: 2016-02-24
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Abstract
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Problems solved by technology

The advantages of direct regeneration of adventitious buds from isolated plant organs do not need to be induced by callus, the culture period is short, and the explants are not easy to mutate during the culture process, which has been reported in other plants, but the direct regeneration of adventitious buds from isolated tung tree tissues has been reported. Buds are rare

Method used

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  • A method for directly regenerating plants from tung leaf petioles
  • A method for directly regenerating plants from tung leaf petioles
  • A method for directly regenerating plants from tung leaf petioles

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Experimental program
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Embodiment 1

[0046] Do the following in order:

[0047] 1. Select mature tung tree seeds of the same year, remove the seed coat, rinse the seed kernels with tap water for 5-8 minutes, then soak for 15-20 hours, change the water 3-4 times in the middle, and then use 0.1% HgCl 2 Disinfect for 3-5 minutes, rinse with sterile water 4-6 times, inoculate in 1 / 2 MS basic medium, culture in dark for 3-5 days, then transfer to light and cultivate for 20-30 days to grow into seedlings; culture The temperature is 26±1°C, the light intensity is 2100-2200lx, and the light time is 12-14h / d;

[0048] Table 2 Effect of different disinfection treatment methods on the disinfection effect of tung tree seed embryos

[0049] 75% alcohol 0.1% mercury chloride pollution rate browning rate 0 2 26.65 0 0 3 3.83 0 0 4 3.03 0 0 5 2.78 1.27 10 3 3.05 4.54

[0050] 10 5 0 49 20 5 0 54 30 5 0 67

[0051] 2. Select the petiole of th...

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Abstract

The invention discloses a method for efficiently and directly reproducing plants by virtue of vernicia fordii petioles, and belongs to the field of plant cell engineering. The method mainly comprises the processes of direct induction of adventitious buds by virtue of the vernicia fordii petioles, secondary reproduction culturing, strong seedling culturing, rooting culturing, acclimatization and transplantation and the like. According to the method, means is provided for the rapid reproduction of high-quality vernicia fordii plants, a solid foundation is also laid for improving the resistance, the yield of vernicia fordii and characters such as oil quality and the like by virtue of a genetic engineering method and establishing a vernicia fordii genetic system, and many means and technical support are provided for the culturing of transgenic vernicia fordii.

Description

technical field [0001] The invention belongs to the field of tung tree cell engineering, and in particular relates to a method for directly regenerating plants from tung tree petioles. Background technique [0002] Vernicia fordii is the general name of Euphorbiaceae (Euphorbiaceae) genus Vernicia. It is an important industrial oil tree species. It is also called the four major woody oil tree species in my country together with camellia oleifera, walnut and Chinese tallow tree. The oil extracted from the seeds of tung oil is called tung oil, which is the highest quality plant drying oil in the world, and is an excellent raw material for paints and printing inks. Tung oil has excellent characteristics such as fast drying, strong adhesion, light specific gravity, rich luster, acid and alkali resistance, high temperature resistance, frost crack resistance, and water resistance. In addition, tung oil is also an excellent coating and the basic raw material for the synthesis of n...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 李泽谭晓风张琳卢锟龙洪旭袁军林青吴玲利曾艳玲
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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