Serum-free protein-free culture medium supporting CHO (Chinese Hamster Ovary Cell) high density suspension culture
A protein-free medium and suspension culture technology, applied in tissue culture, microorganisms, biochemical equipment and methods, etc., can solve the problems of being easily affected by physical and chemical factors, pollution separation and purification, and high cost, and achieve the goal of being conducive to separation and purification. , the risk of contamination is small, the effect of chemical composition is clear
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[0038] Example 1:
[0039] CHO cell serum-free and protein-free medium dry powder components and consumption of the present invention are as follows:
[0040]
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[0044] Preparation method of medium dry powder (1L):
[0045] Add 1L of dry powder to 900ml of ultrapure water, the temperature is about 35℃, after stirring for half an hour, add a certain amount of sodium hydroxide to help dissolve, then add concentrated hydrochloric acid and sodium bicarbonate to adjust the pH to 6.9~7.4. Filter with 0.2um negative pressure and store in 500ml glass bottles aseptically protected from light.
[0046] The physicochemical property parameters, detection method and detection results of the CHO cell serum-free and protein-free culture medium obtained by the present invention are shown in Table 1:
[0047] Table 1
[0048] Test items
[0049] From the test results, the configured liquid medium is qualified and can be used for large-scale s...
Example Embodiment
[0050] Example 2:
[0051] Insulin and transferrin were added on the basis of the serum-free and protein-free medium of Example 1, and the dry powder components and consumption of its medium were as follows:
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Example Embodiment
[0055] Example 3:
[0056] On the basis of the serum-free and protein-free medium of Example 1, the hydrolyzate (molecular weight less than 1000 Daltons) of plant origin after ultrafiltration was added, and the dry powder components and consumption of the medium were as follows:
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