Gene circuit structure for realizing glioma marker detection

A brain glioma and gene circuit technology, applied in the field of synthetic biology, can solve the problems of high cost and experimental requirements, and achieve the effect of simple structure, strong anti-interference ability, and overcoming interference.

Active Publication Date: 2014-10-15
BEIHANG UNIV
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Problems solved by technology

The traditional methods for detecting tumor markers are serum testing, tissue sections, and i

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  • Gene circuit structure for realizing glioma marker detection
  • Gene circuit structure for realizing glioma marker detection
  • Gene circuit structure for realizing glioma marker detection

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Embodiment Construction

[0020] See figure 1 , figure 2 , image 3 As shown, a gene circuit structure for realizing the detection of glioma markers is implemented as follows: it consists of a single index detection module and a fusion circuit module, and the two modules are sequentially connected to each other. The single index detection module is composed of an inverter. The inverter is composed of an input terminal, an output terminal and an inverting module, and its main function is to invert the input signal and output the inverse signal. This module detects the three glioma markers GFAP, YKL-40 and CD133 respectively, the input terminals are In1, In2 and In3 respectively, and the corresponding output terminals are OUT1, OUT2 and OUT3 respectively. The fusion circuit module is composed of a three-port input NAND gate. The expression of GFP (green fluorescent protein) is regulated by the input of three proteins A, B, and C. When the concentrations of A, B, and C are high, the expression of GFP...

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Abstract

The invention relates to a gene circuit structure for realizing glioma marker detection. The gene circuit structure consists of a single-index detection module and an integration circuit module. The two modules are in sequential connection mutually. The single-index detection module is composed of a phase inverter, which consists of an input end, an output end and a reverse module and is used for negating an input signal and outputting a reverse signal. The integration circuit is composed of a three-port input NAND gate, which is used for performing NAND operation on the input. When the three inputs are all at a high level, a low level signal is output by the circuit. The gene circuit structure provided by the invention has the advantages of simple structure, fast response, stable output and good performance, overcomes the interference from independent work of a single module and other unfavorable factors, and realizes glioma marker detection and alarming functions. The gene circuit structure has good practical value and broad application prospects in the technical field of synthetic biology.

Description

technical field [0001] The invention relates to a gene circuit structure for realizing the detection of brain glioma markers, and belongs to the technical field of synthetic biology. Background technique [0002] Glioma (glioma) includes a variety of different types of malignant brain tumors, accounting for about 50% to 60% of human primary brain tumors, with high morbidity and mortality. Gliomas of the human brain become more common with age. The increase in permeability of brain gliomas correlates with their malignancy. Malignant gliomas are characterized by their tendency to invade and recur easily. [0003] A class of macromolecular substances closely related to the occurrence of cancer tumors is called tumor markers (Tumor Markers). Tumor markers can be detected from blood or body fluids, and their components are mainly macromolecular proteins, including tumor-associated antigens, ectopic hormones, enzymes, and changes in the host anti-tumor immune cascade. There ar...

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Application Information

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IPC IPC(8): G01N27/26
Inventor 王翔曾梓臻陈瀚王维克杜培刘金静
Owner BEIHANG UNIV
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