Tissue culture method for early-maturing pear stem section and culture medium

A technology for tissue culture and inoculation of medium, applied in the field of plant cell molecular biology, can solve the problems of average rooting rate, low average root number, low proliferation coefficient of test-tube plantlets, low reproduction coefficient, etc., to reduce browning rate, shorten Growth time, effect of increasing proliferation coefficient

Active Publication Date: 2014-11-05
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] When Sucui 1 and Sucui 2 were propagated by conventional cutting methods, the reproduction coefficients were low and highly heterozygous; when conventional tissue culture methods were...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Tissue culture of pear stem segments of 'Su Cui No. 1' and 'Su Cui No. 2' was carried out as follows.

[0049] Collection and disinfection of explants: From October to December of the previous year, annual shoots were collected from the orchard and stored in a 4°C refrigerator. From late February to early March of that year, the branches were taken and inserted into sterile water, at 22°C-25°C, 8 hours of light per day, 16 hours of darkness, light intensity of 1500-2000lx, germination was accelerated, and fresh sterile water was changed every 7 days. In early and mid-April, after the buds germinate, take multiple stem sections with buds, cut them into 1-1.5cm high stem sections with one bud, and perform the following disinfection treatment: use 100mg / L carbenzyl carboxybenzyl After soaking in tap water with penicillin for 2.5 hours, vortex for 5-8 minutes with a mixed disinfectant solution, then peel off the bracts, and perform secondary disinfection with a mixed disinf...

Embodiment 2

[0081] Example 2 Adopt conventional tissue culture rapid propagation method to cultivate 'Su Cui No. 1' and 'Su Cui No. 2'

[0082] The following methods were used to cultivate 'Su Cui No. 1' and 'Su Cui No. 2':

[0083] 1) Collection and disinfection of explants: select robust annual branches from the field, cut young branches as explants, rinse with washing powder, and rinse with tap water for 30 minutes. On the ultra-clean workbench, the treated explants were sterilized with 75% alcohol for 20 seconds, 0.1% mercuric chloride for 8-10 minutes, and rinsed with sterile water for 4-5 times. The scales were removed with a scalpel, and the naked buds were cut and inoculated on the induction medium, and 3 explants were inoculated in each bottle. The induction medium is: add 6-BA, IBA, GA to 1 / 2MS medium 3 , sucrose and agar, wherein the final concentration of 6-BA is 1.0mg / L, the final concentration of IBA is 0.2mg / L, GA 3 The final concentration of sucrose is 2.0mg / L, the fina...

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Abstract

The invention provides a tissue culture method for an early-maturing pear stem section and a culture medium, and belongs to the technical field of plant cell molecule biology. The tissue culture method comprises the following steps of performing induced differentiation culture on an explant through an initial inoculation culture medium to obtain a primary tissue culture seedling; performing multiplication culture on the primary tissue culture seedling through a multiplication culture medium, then cutting off new branches, transferring into a seedling enhancing culture medium for subculture, and transferring into a rooting culture medium A and a rooting culture medium B for rooting culture. The culture medium for the tissue culture of the early-maturing pear stem section is formed by one or more than two of the initial inoculation culture medium, the multiplication culture medium, the seedling enhancing culture medium and the rooting culture medium. According to the tissue culture method disclosed by the invention, a disinfection method is safe and short in time; the multiplication coefficient, the rooting rate, the average root length and the average root number of the tissue culture seedlings are obviously increased.

Description

technical field [0001] The invention belongs to the field of plant cell molecular biology, and in particular relates to a tissue culture method and culture medium for pear stem segments. Background technique [0002] Sucui No. 1 is a high-quality early-maturing sand pear. The parent is Huasu × Cuiguan. It was crossed in 2003, and the hybrid seedlings were cultivated in 2004. In 2011, it was approved by the Jiangsu Provincial Crop Variety Approval Committee (Su Jianguo 201107). The pulp is white, fine and crisp, with few or no stone cells, juicy and sweet, with good flavor and good quality. It is easy to form flower buds, has strong continuous fruiting ability, high fruit setting rate, and the fruit ripening period is early July. [0003] Sucui No. 2 is also an early-maturing sand pear. The parent is Xizilu × Cuiguan. It was crossed in 2003, and the hybrid seedlings were cultivated in 2004. It passed the variety approval of Jiangsu Province in 2011. The fruit is obovate, wi...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 王宏蔺经常有宏马娜甘慧芳
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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