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Low-temperature storage method for artificial propagation of harmonia axyridis

The invention relates to a technology for cryopreservation of lady beetles, which is applied in the field of low temperature storage of artificially propagated and reproduced adults of lady beetles, and can solve the problems of prolonged storage time and the inability of the biological characteristics of lady beetles to satisfy long-term storage and transportation, and the like. To achieve the effect of improving the survival rate

Inactive Publication Date: 2014-11-19
WEIFANG UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a low-temperature storage method for the artificially multiplied generation of Heterochromia which can prolong the storage time and has a high survival rate of Heterochromia after low-temperature storage. , to solve the problem that the biological characteristics of the heterochromia can not meet the long-term storage and transportation, so as to give full play to the role of the natural enemy in the biological control of pests

Method used

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  • Low-temperature storage method for artificial propagation of harmonia axyridis
  • Low-temperature storage method for artificial propagation of harmonia axyridis
  • Low-temperature storage method for artificial propagation of harmonia axyridis

Examples

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Effect test

Embodiment 1

[0021] In order to exclude the influence of cold-shock on the low-temperature survival of H. heterochromia, the artificially multiplied H. heterochromia adults were exposed at 10°C for 24 h, and then moved into a colorless translucent plastic box (15 cm × 10 cm × 7 cm ), the plastic box is covered with a parafilm, and a number of ventilation holes are pierced on the parafilm.

[0022] The Heterochromia was moved into a plastic petri dish and stored under low temperature exposure at 0°C for 1 week, in which the high temperature exposure was intermittently exposed at 5°C for 0.5h every day.

[0023] After the end of low-temperature and intermittent high-temperature storage, H. heterochromia was transferred to rearing conditions to recover for 24 hours, and the surviving adults with walking coordination were screened.

Embodiment 2

[0025] In order to get rid of the impact of cold shock (cold-shock) on the low-temperature survival of ladybugs, artificial propagation (the breeding condition of artificial propagation is 24 ℃ at temperature, humidity is 60%, photoperiod L:D=16h :8h) H. heterochromia adults were exposed at 10°C for 24h, and moved into a colorless translucent plastic box (15cm×10cm×7cm). air vent.

[0026] Heterochromia was moved into a plastic petri dish and stored at -2°C for four weeks, with intermittent high temperature exposure at 15°C for 1 hour every day.

[0027] After storage at low temperature and intermittent high temperature, the ladybugs were transferred to rearing conditions (T=24°C, photoperiod L:D=16h:8h) to recover for 24 hours, and surviving adults with coordinated walking were screened.

Embodiment 3

[0029] In order to get rid of the impact of cold shock (cold-shock) on the low-temperature survival of ladybugs, artificial propagation (the breeding condition of artificial propagation is that temperature is 26 ℃, humidity is 70%, photoperiod L:D=16h :8h) H. heterochromia adults were exposed at 10°C for 24h, and moved into a colorless translucent plastic box (15cm×10cm×7cm). air vent.

[0030] The Heterochromia ladybugs were moved into plastic petri dishes and stored under low temperature exposure at 5°C for 2 weeks, with intermittent high temperature exposure at 30°C for 2 hours every day.

[0031] After the H. heterochromia has been stored at low temperature and intermittent high temperature, it is transferred to the feeding condition (T=26°C, photoperiod L:D=16h:8h) to recover for more than 24 hours, and the surviving adults with coordinated walking are screened.

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Abstract

The invention discloses a low-temperature storage method for artificial propagation of harmonia axyridis. The low-temperature storage method comprises the following steps: (1) exposing adult harmonia axyridis obtained through artificial propagation at 10 DEG C for twenty-four hours, and putting the adult artificial propagation in a ventilating device; (2) exposing and storing the harmonia axyridis obtained in the step (1) at -5 DEG C -5 DEG C, wherein interrupted high temperature exposure is conducted on the harmonia axyridis for 0.5-4 hours at 5 DEG C -30 DEG C everyday; (3) after low temperature storage and interrupted high temperature storage of the harmonia axyridis obtained in the step (2) are completed, transferring the harmonia axyridis into the feeding conditions and recovering the harmonia axyridis for more than twenty-four hours, and screening survival adult harmonia axyridis, wherein the artificial propagation conditions are as follows: temperature ranges from 24DEG C to 26 DEG C, humidity ranges from 60 percent to 70 percent, and the photoperiod L:D=16h:8h. By means of the low-temperature storage method, low temperature storage and interrupted high temperature storage are conducted on the harmonia axyridisd. Compared with constant low temperature storage, the survival rate can be remarkably increased.

Description

technical field [0001] The invention relates to the technical field of storage of ladybug heterochromia, in particular to a low-temperature storage technology for artificially multiplied generation of ladybird heterochromia adults. Background technique [0002] The long-term use of chemical pesticides has brought about a series of ecological environment and food safety problems, and the control of chemical pesticide pollution not only requires a gradual reduction in its use, but also requires the search for technologies that optimize, upgrade and replace traditional control methods. Among them, the biological control technology based on the release of natural enemy insects is playing an increasingly important role in integrated pest management (IPM). The first prerequisite for mass release of natural enemy insects is to accumulate a sufficient amount of products within a certain period of time, but the storage time of most natural enemy insects is generally short, and the in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033A01N1/00
Inventor 赵静夏海波李晓莉李艳青潘好琴
Owner WEIFANG UNIV OF SCI & TECH
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