Ginkgo biloba leaf extract as well as preparation method and application thereof

A technique for Ginkgo biloba leaf and extract, applied in the field of Ginkgo biloba leaf extract and preparation thereof, can solve the problems of lack of development and utilization of Ginkgo biloba proanthocyanidins, low content of proanthocyanidins, etc., and achieve the effects of good pharmacological activity, simple operation and fast speed.

Active Publication Date: 2014-11-19
ZHEJIANG CONBA PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] But so far, the development and utilization of proanthocyanidins in Ginkgo biloba is relatively lacking. Most of the literature research is still focused on improving the content of total flavonoids and lactones. The content of proanthocyanidins in the extract is still low. For example, CN1660224 discloses a grape extract 1-99%, Ginkgo biloba extract 1-99% antioxidant composition, grape extract contains proanthocyanidins (proanthocyanidins in grape seeds are different from those in ginkgo leaves, the latter has stronger antioxidant capacity than the former) and resveratrol, Ginkgo biloba extract contains flavonoids, or contains flavonoids and lactones at the same time; CN100421680 discloses Ginkgo biloba extract and its preparation method and application, Ginkgo total flavonoids in Ginkgo biloba extract> 64%, total ginkgo lactones>11%, proanthocyanidins containing 7-10%

Method used

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  • Ginkgo biloba leaf extract as well as preparation method and application thereof
  • Ginkgo biloba leaf extract as well as preparation method and application thereof
  • Ginkgo biloba leaf extract as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: Determination of content of proanthocyanidins, total flavonoids, total flavonol glycosides, and total terpene lactones

[0028] 1. Determination of proanthocyanidin content

[0029] 1.1 The test solution: Accurately weigh 100mg of the sample powder, put it in a 50ml volumetric flask, add 30mL of methanol precisely, treat it with ultrasound for 20min, let it cool to room temperature, add methanol to the scale, shake well, take the supernatant, and get .

[0030] 1.2 Preparation of standard curve of proanthocyanidin reference substance: take 30mg of proanthocyanidin reference substance, weigh it accurately, put it in a 50ml volumetric flask, add 30mL of methanol precisely, treat it with ultrasonic for 20min, let cool to room temperature, add methanol to the scale, shake well, absorb proanthocyanidin control Product solution 0.0, 1.0, 2.0, 3.0, 4.0, 5.0mL, respectively put in 10mL measuring bottle, add methanol to the mark, shake well. Take 1ml into a 10ml ...

Embodiment 2

[0046] Embodiment 2: the selection of extraction solvent

[0047] Take by weighing 15g ginkgo leaf, put 250ml eggplant type bottle, add 7 times of amount extraction solvent (30% ethanol, 40% ethanol, 50% ethanol, 60% ethanol, 70% ethanol, 80% ethanol, 90% ethanol) respectively, respectively Reflux extraction 3 times, each extraction 1h, determination of proanthocyanidins, total flavonoids, total flavonol glycosides and total terpene lactone transfer rate.

[0048] Table 1 The influence of extraction solvent on the transfer rate of active ingredients

[0049]

[0050] From the results in the above table, it can be seen that when 60% ethanol is extracted, the comprehensive extraction efficiency of various active ingredients is the highest, so 60% ethanol is selected as the extraction solvent.

Embodiment 3

[0051] Embodiment 3: the selection of solid-liquid ratio

[0052] Weigh 15g ginkgo leaves, put them in a 250ml eggplant-shaped bottle, add 3 times, 5 times, 7 times, 10 times the amount of 60% ethanol, reflux extraction 3 times, each extraction 1h, measure proanthocyanidins, total flavonoids, total flavonol glycosides and total terpene lactone transfer rate.

[0053] The influence of table 2 solid-liquid ratio on active ingredient transfer rate

[0054]

[0055] From the results in the above table, it can be concluded that the extraction effect is the best when the amount of solvent for each extraction is 7 times the amount of crude drug.

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Abstract

The invention discloses a ginkgo biloba leaf extract as well as a preparation method and application thereof. In the ginkgo biloba leaf extract, the content of total ginkgolides is 6-12 percent (w / w), the content of total flavonoids is 30-70 percent (w / w), the content of proanthocyanidins is 20-60 percent (w / w), 6-12 percent of total ginkgolides plus 30-70 of total flavonoids plus 20-60 percent of proanthocyanidins is more than 90 percent, the content of total flavonoid glycosides is 20-50 percent (w / w), and the ginkgo biloba leaf extract can be used for preparing food, health food or drugs for preventing or treating nervous system diseases like diabetic cataract, diabetic optic neuropathy or diabetic retinal lipidemia. The preparation method of the ginkgo biloba leaf extract comprises the following steps: extracting ginkgo biloba leaves from ethanol water solution, concentrating the extracting solution to thick paste, digesting the thick paste with cold water, filtering, absorbing and separating the filtrate with macroporous resin, and concentrating and drying eluant to obtain the ginkgo biloba leaf extract.

Description

technical field [0001] The invention relates to natural plant extracts, in particular to a ginkgo biloba extract and its preparation method and application. Background technique [0002] The active ingredients of Ginkgo biloba extract recorded in the pharmacopoeias of various countries are flavonoids and lactones. For example, the first edition of "Chinese Pharmacopoeia" in 2010 recorded the content of Ginkgo biloba extract. Less than 0.40%, 0.25%. However, in recent years, more and more reports suggest that proanthocyanidins are one of the components with the strongest antioxidant capacity in the plant kingdom, and they have a good effect on the prevention and treatment of cardiovascular and cerebrovascular diseases and nervous system diseases. Moreover, ginkgo leaves contain proanthocyanidins, which are rich in resources, and their content in dried ginkgo leaves can reach 0.5% to 4%, or even higher. Therefore, the simultaneous extraction of flavonoids, lactones and proan...

Claims

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Application Information

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IPC IPC(8): A61K36/16A23L1/29A61P25/00A61P3/10A61P27/02A61P9/10A61P27/12A61K127/00A23L33/00
Inventor 乔洪翔王如伟胡江宁陈琳何厚洪瞿伟陈玲芳吕丽丽
Owner ZHEJIANG CONBA PHARMA
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