Construction method and application of maintainer line of hybrid scallops of purple scallops and Argopecten irradians concentricus

Abstract

The invention relates to a construction method and application of the maintainer line of hybrid scallops of purple scallops and Argopecten irradians concentricus. The maintainer line is Argopecten irradians concentricus strains capable of enabling the generations of the male sterility line of the hybrid scallops of the purple scallops and the Argopecten irradians concentricus to have the characteristics of male sterility and ovum fertility, and the hybrid scallops of the purple scallops and the Argopecten irradians concentricus are the hybrid generations of purple scallop ova and Argopecten irradians concentricus sperms. The construction method comprises the steps that firstly, a large number of the first filial generations of the hybrid scallops of the purple scallops and the Argopecten irradians concentricus are constructed, and male sterile individuals are found out from the first filial generations for breeding of the male sterility line; then sperms of male parent Argopecten irradians concentricus are used for continuous backcross, and the combinations of the male sterility line-maintainer line with all generations of male sterility individuals are respectively bred; finally, the male sterility line parent scallops are produced through hybridization of the male sterility line and the maintainer line, and the maintainer line is used for producing commodity offspring seeds of the hybrid scallops of the purple scallops and the Argopecten irradians concentricus. According to the construction method and application, the maintainer line of the scallops is firstly built in animals, and the obstacles of autofertilization of hermaphrodite scallops in the hybridization process are effectively eliminated.

Description

technical field [0001] The invention belongs to shellfish breeding technology-a method for cultivating commercial hybrid scallop seedlings, and in particular relates to the construction and application of a maintainer line of Zimo hybrid scallop (hybrid progeny of purple scallop ova and Mexico gulf scallop sperm). Background technique [0002] The Gulf of Mexico scallop (Argopecten irradians concentricus) is native to the coast of the Gulf of Mexico in the United States and was introduced to my country in the 1990s. The Gulf of Mexico scallop grows fast and adapts to a wide range of temperatures. It is especially suitable for high-temperature sea areas and has strong stress resistance. It is very suitable for farming in the southern seas of my country. It is currently one of the main scallop breeding species in the southern seas. However, due to the long-term inbreeding after the introduction, the germplasm of the Gulf of Mexico scallop has been seriously degraded during the...

AI Technical Summary

Problems solved by technology

[0006] However, in animal breeding, there have been no reports of male sterile and female fertile individuals at home and abroad so far, so it is impossible to establish male sterile lines, maintainer lines and restorer lines similar to crops for hybrid breeding