Construction method and application of maintainer line of hybrid scallops of purple scallops and Argopecten irradians concentricus

A construction method and technology of maintainer lines, applied in the construction and application field of purple ink hybrid scallop maintainer lines, can solve the problems of inability to establish male sterile lines, maintainer lines and restorer lines, and achieve the effect of overcoming the obstacles of self-fertilization

Active Publication Date: 2014-11-26
台州市金瓯海洋科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, in animal breeding, there have been no reports of male sterile and female fertile individuals at home and abroad so

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1) Screening of male sterile individuals of purple ink hybrid scallops and breeding of male parents: 50 purple scallops with mature gonads and 50 Gulf of Mexico scallops were selected in the first year, and each scallop was placed in the pre- Add seawater at 23°C to a separate container to spawn and ejaculate, collect the eggs of each purple scallop and the sperm and eggs of each gulf scallop individually, add a small amount of egg suspension from a single purple scallop to each purple scallop For the semen of the Gulf of Mexico scallop, stir to mix the sperm and eggs evenly, observe under a microscope that there are 5-6 sperm around each egg, and place the fertilized eggs in a dark environment to hatch, that is, to obtain the first-generation family of purple ink hybrids. Use the above method to establish at least 20 such hybrid families, and at the same time, mix and fertilize the sperm and eggs of Gulf scallops for fertilization to establish a corresponding number of ...

Embodiment 2

[0025] In 2011, using the imported purple scallop and the Gulf of Mexico scallop from Zhanjiang, Guangdong, a first-generation orthogonal and a reciprocal family of interspecific hybrids were co-bred. According to actual measurements, the average shell height of Zimo hybrid scallops (the hybridization of purple scallop eggs and Gulf scallop sperm) is 52.38 mm, the weight is 27.40 grams, and the column weight is 2.46 grams. The body weight is 14.80 grams, and the column weight is 1.13 grams. Compared with the Gulf of Mexico scallop of the same period, the first generation of Zimo hybrids had a shell height of 24.7%, a body weight of about 85.1%, and a column weight of 117.7%. In the spring of 2012, after promoting the maturation of the first-generation hybrid scallops, select scallops that are large and only develop in the female area. After drying in the shade for 30 minutes, place the scallops in a container filled with seawater at 23 degrees to stimulate spawning. Use a sing...

Embodiment 3

[0028] In 2014, a total of 14 first-generation hybrid families of purple scallop eggs and Gulf scallop sperm were established using imported purple scallops and Gulf scallops, and self-bred families of Gulf scallops for sperm were established, according to the method in Example 1 The male sterile line of Zimo hybrid scallop and its corresponding maintainer and restorer lines can be bred within 1-3 years to realize the commercial production of Zimo hybrid scallop seedlings.

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Abstract

The invention relates to a construction method and application of the maintainer line of hybrid scallops of purple scallops and Argopecten irradians concentricus. The maintainer line is Argopecten irradians concentricus strains capable of enabling the generations of the male sterility line of the hybrid scallops of the purple scallops and the Argopecten irradians concentricus to have the characteristics of male sterility and ovum fertility, and the hybrid scallops of the purple scallops and the Argopecten irradians concentricus are the hybrid generations of purple scallop ova and Argopecten irradians concentricus sperms. The construction method comprises the steps that firstly, a large number of the first filial generations of the hybrid scallops of the purple scallops and the Argopecten irradians concentricus are constructed, and male sterile individuals are found out from the first filial generations for breeding of the male sterility line; then sperms of male parent Argopecten irradians concentricus are used for continuous backcross, and the combinations of the male sterility line-maintainer line with all generations of male sterility individuals are respectively bred; finally, the male sterility line parent scallops are produced through hybridization of the male sterility line and the maintainer line, and the maintainer line is used for producing commodity offspring seeds of the hybrid scallops of the purple scallops and the Argopecten irradians concentricus. According to the construction method and application, the maintainer line of the scallops is firstly built in animals, and the obstacles of autofertilization of hermaphrodite scallops in the hybridization process are effectively eliminated.

Description

technical field [0001] The invention belongs to shellfish breeding technology-a method for cultivating commercial hybrid scallop seedlings, and in particular relates to the construction and application of a maintainer line of Zimo hybrid scallop (hybrid progeny of purple scallop ova and Mexico gulf scallop sperm). Background technique [0002] The Gulf of Mexico scallop (Argopecten irradians concentricus) is native to the coast of the Gulf of Mexico in the United States and was introduced to my country in the 1990s. The Gulf of Mexico scallop grows fast and adapts to a wide range of temperatures. It is especially suitable for high-temperature sea areas and has strong stress resistance. It is very suitable for farming in the southern seas of my country. It is currently one of the main scallop breeding species in the southern seas. However, due to the long-term inbreeding after the introduction, the germplasm of the Gulf of Mexico scallop has been seriously degraded during the...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 王春德孙奎府马斌
Owner 台州市金瓯海洋科技有限公司
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