Rapid propagation method for cultivating hygrophila salicifolia suspension cells

A technology of suspended cells and hydrangeas, which is applied in the field of plants and achieves the effects of low production cost, short growth cycle and fast proliferation rate

Inactive Publication Date: 2014-12-03
NANJING TONGZE AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This new type of cell has several technical advantages: it can grow quickly with less expensive materials; there are fewer steps needed for producing this material compared to traditional methods like fermenting or extracting them from plants that produce these substances.

Problems solved by technology

The technical problem addressed by this patented technology is how to create an effective treatment that can stop bleeding quickly without causing damage during surgery while being safe enough at home.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Take the water and freshly pump out the young leaves, remove the surface dirt with a brush, soak in bleach for 2 minutes, rinse with running water for 18 minutes, and disinfect with 10% Antiformin solution + two drops of Tween-80 on the ultra-clean workbench for 10 minutes. Rinse 5-7 times with bacterial water, absorb the surface moisture with absorbent paper, and inoculate the sterilized leaves into N 6 +2,4-D1mg / L+50mg / L cysteine+3% sucrose+0.65% agar for callus induction, light intensity 600lx, light period 5h, dark period 19h, induced water coir raincoat callus Organization AccessN 6 +2,4-D1.0mg / L+6-BA5.0mg / L+3% sucrose+0.65% agar, add 0.005%-0.1% (EMS) for mutagenic proliferation of callus, pH5.8, Illumination 1200lx, temperature 28°C, after proliferation, screen the tender green and loose callus and insert it into liquid medium N 6 +2,4-D0.5mg / L+6-BA4.0mg / L+3% sucrose+0.65% agar+50mg / L yeast, weighed after 30 days, and calculated the proliferation rate.

Embodiment 2

[0011] Take the water and freshly pump out the young leaves, remove the surface dirt with a brush, soak in bleach for 2 minutes, rinse with running water for 18 minutes, and disinfect with 10% Antiformin solution + two drops of Tween-80 on the ultra-clean workbench for 10 minutes. Rinse 5-7 times with bacterial water, absorb the surface moisture with absorbent paper, and inoculate the sterilized leaves into N 6 +2,4-D1mg / L+50mg / L cysteine+3% sucrose+0.65% agar for callus induction, light intensity 600lx, light period 5h, dark period 19h, induced water coir raincoat callus Organization AccessN 6 +2,4-D1.0mg / L+6-BA5.0mg / L+3% sucrose+0.65% agar, add 0.005%-0.1% (EMS) for mutagenic proliferation of callus, pH5.8, Illumination 1200lx, temperature 28°C, after proliferation, screen the tender green and loose callus and insert it into liquid medium N 6 +2,4-D1.0mg / L+6-BA5.0mg / L+3% sucrose+0.65% agar+80mg / L yeast, weighed after 30 days, and calculated the proliferation rate.

Embodiment 3

[0013] Take the water and freshly pump out the young leaves, remove the surface dirt with a brush, soak in bleach for 2 minutes, rinse with running water for 18 minutes, and disinfect with 10% Antiformin solution + two drops of Tween-80 on the ultra-clean workbench for 10 minutes. Rinse 5-7 times with bacterial water, absorb the surface moisture with absorbent paper, and inoculate the sterilized leaves into N 6 +2,4-D1mg / L+50mg / L cysteine+3% sucrose+0.65% agar for callus induction, light intensity 600lx, light period 5h, dark period 19h, induced water coir raincoat callus Organization AccessN 6 +2,4-D1.0mg / L+6-BA5.0mg / L+3% sucrose+0.65% agar, add 0.005%-0.1% (EMS) for mutagenic proliferation of callus, pH5.8, Illumination 1200lx, temperature 28°C, after proliferation, screen the tender green and loose callus and insert it into liquid medium N 6 +2,4-D1.0mg / L+6-BA4.0mg / L+3% sucrose+0.65% agar+60mg / L yeast, weighed after 30 days, and calculated the proliferation rate.

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PUM

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Abstract

The invention provides a rapid propagation method for cultivating hygrophila salicifolia suspension cells. The method comprises the following steps: obtaining of sterile blades, induction of calluses, induced mutation proliferation of the calluses, cultivation of suspension cells; and the like. The hygrophila salicifolia suspension cells prepared by the method disclosed by the invention are fast in split growth speed, and high in yield, and a lot of suspension cells with high medicinal secondary metabolite content can be obtained.

Description

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Claims

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Application Information

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Owner NANJING TONGZE AGRI SCI & TECH
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