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A method for indirect determination of 1,3-dioleic acid-2-palmitic acid triglyceride content

A technology of palmitic acid triglyceride and ‐dioleic acid, applied in the field of chemical analysis, can solve the problems of interfering with the effective performance of products, affecting the accuracy of OPO, etc., to achieve the effect of supporting healthy growth and meeting nutritional requirements

Active Publication Date: 2017-12-12
中国检验检疫科学研究院综合检测中心 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These OOPs and POOs will affect the accuracy of the OPO test results and interfere with the judgment of the effective performance of the product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The first step of indirect detection of OPO:

[0042] Analyzing the dairy mixture gives:

[0043] 1. Total fatty acid composition

[0044] 2. sn-2 fatty acid composition

[0045] Using GB / T24894-2010 as the detection method, the result is:

[0046] Overall %P = 3 / 9 = 33.3%;

[0047] %P of sn-2 bits = 1 / 3 = 33.3%

[0048] Overall %O = 6 / 9 = 66.7%

[0049] %O = 2 / 3 = 66.7% for the sn-2 position.

Embodiment 2

[0051] The second step of indirect detection of OPO:

[0052] Calculated from the result:

[0053] 1. Content of sn-2 P in total fat

[0054] 2. Content of sn-1+3 in total fat

[0055] (1) Proportion of sn-2 P in total fat:

[0056] Because the P in the sn-2 position accounts for 1 / 3 of the total fatty acids, the ratio of the P in the sn-2 position to the total fat is: 33.3% / 3=11.1%.

[0057] (2) Proportion of sn-1+3 O in total fat:

[0058] The proportion of sn-2 O in total fat: 66.7% / 3=22.25%.

[0059] (total %O)-(%O in sn-2 position)=%O in sn-1+3 position, so the proportion of sn-1+3 position O in total fat: 66.7%-22.25%=44.4%

Embodiment 3

[0061] The third step of indirect detection of OPO:

[0062] based on:

[0063] sn-2 P in total fat content: 11%

[0064] sn-1+3 O in total fat content: 44.4%

[0065] Calculate OPO:

[0066] According to sn-2 bit P

[0067] According to sn-1+3 bit O

[0068] We choose a relatively low value as the limiting factor

[0069] OPO calculated from P:

[0070] Every 1% of the P content of the sn-2 position must correspond to 3% of the OPO content,

[0071] That is: P content at sn-2 position×3=11.1%×3=33.3%.

[0072] Calculate OPO according to O:

[0073] Every 1% of sn-1+3 O must correspond to 1.5% of OPO content,

[0074] Namely: O content at sn-1+3 position×1.5=44.4%*1.5=66.7%.

[0075] OPO content calculated from P is 33.3%

[0076] OPO content calculated from O is 66.7%

[0077] Because 33.3%<66.6%, the OPO content is 33.3%, even if the indirect method is used to measure the digested substances in the form of OPO, the result is correct.

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Abstract

The invention relates to the technical field of chemical analysis, and in particular relates to a method for indirectly measuring the content of 1,3-dioleoyl-2-palmitoyl triglyceride (OPO). The method is used for directly measuring the active part of the OPO, so as to further measure the content of the active OPO. The method can directly reflect the effective performance of the OPO, and the content of the OPO directly corresponds to the effective performance of the OPO used as a food additive. According to the method for indirectly measuring a mode of digested OPO, can easily distinguish P at sn-2 bit, can easily identify non geometric isomers of the P at the sn-2 bit and can prevent counterfeiting.

Description

technical field [0001] The invention relates to the technical field of chemical analysis, in particular to an indirect determination method for the content of 1,3-dioleic acid-2-palmitic acid triglyceride in dairy products. Background technique [0002] With the development of the economy and the improvement of living standards, the consumption of food for infants and young children has become higher and higher. Infants and young children are small and their physiological functions are immature. However, in order to achieve normal growth and development, the calorie requirement per kilogram of body weight is more than twice that of adults. If carbohydrates and protein are mainly used to supply energy, each gram can only supply 16KJ of calories, so the amount of food must be quite large, which is not a small burden for babies whose digestive function is not very perfect; while fat can supply more than twice the calories of protein or sugar, that is, 38KJ of calories per gram,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/04
Inventor 刘汉霞张凤霞马小宁吴博隆仲维科G·本迪罗
Owner 中国检验检疫科学研究院综合检测中心
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