Blended copolymer gel with capillary self-coating effect and preparation method thereof

A copolymer and capillary technology, which is applied in the field of blended copolymer gel and its preparation, can solve the problems that the uniform distribution of the two polymers cannot be guaranteed, the coating ability of the random copolymer is different, and the product quality is difficult to guarantee.

Active Publication Date: 2013-03-27
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, random copolymerization is random, and the uniform distribution of the two polymers cannot be guaranteed. The coating ability of random copolymers synthesized in different batches may be different, so the quality of the product is difficult to guarantee

Method used

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  • Blended copolymer gel with capillary self-coating effect and preparation method thereof
  • Blended copolymer gel with capillary self-coating effect and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1, the preparation of self-coating polymer (polyN, N-dimethylacrylamide)

[0027] Add 5g of N,N-dimethylacrylamide monomer, 13mL of methanol, 37mL of deionized water, 5mg of ammonium persulfate (APS) and a stirring bar into a 250mL round bottom flask, and pass helium for 1 hour to remove the oxygen in the system . Then 5 μL of N,N,N',N'-tetramethyldiethylamine (TEMED) was added via a syringe, and reacted at room temperature for 18 hours. After the reaction, 20 mL of water was added, and then the polymer was precipitated with ice acetone, then dissolved in water, dialyzed for a week (with a dialysis membrane with a molecular weight cut-off of 7000), and then freeze-dried to obtain a white solid. The viscosity-average molecular weight of polymer measured by Ubbelohde viscometer is 300,000.

Embodiment 2

[0028] Example 2, Preparation of a sieve polymer (poly N, N-dimethylacrylamide-acrylamide random copolymer) polymerized with self-coating polymer monomers

[0029] Add 1g of N,N-dimethylacrylamide monomer, 10g of acrylamide monomer, 80mL of deionized water, 10mg of ammonium persulfate (APS) and a stirring bar into a 250mL round bottom flask, and pass helium for 1 hour to remove the system Oxygen in. Then 10 μL of N,N,N',N'-tetramethyldiethylamine (TEMED) was added via a syringe, and reacted at room temperature (25°C) for 24 hours. After the reaction, the polymer was precipitated with acetone, dissolved in water, dialyzed for a week (with a dialysis membrane with a molecular weight cut-off of 7000), and then freeze-dried to obtain a white solid. The viscosity-average molecular weight of the polymer measured with an Ubbelohde viscometer is 1.4 million (the test conditions are tested according to the method for polyacrylamide, and the national standard GB 12005.1 is used for the...

Embodiment 3

[0030] Embodiment 3, the preparation of blend copolymer gel

[0031] 1. Obtaining the sol buffer

[0032] 1. Measure 5ml of sterile water in a clean 20ml glass bottle.

[0033] 2. Weigh 3.8g of urea (ultra-pure grade). Add urea to a bottle containing water. Cap and shake gently by hand until all the urea is dissolved.

[0034]3. Add 1ml of 10-fold Running Buffer (A.C.E Buffer product number K761 purchased from Amresco, USA), and shake gently to obtain 10ml of sol buffer.

[0035] 2. Copolymer blend gel (sol buffer: poly N, N-dimethylacrylamide: poly N, N-dimethylacrylamide-acrylamide random copolymer = 500ml: 1g: 10g)

[0036] 4. Weigh 0.02g of the poly-N,N-dimethylacrylamide of Example 1 and add it to the bottle,

[0037] 5. Weigh 0.2g poly N,N-dimethylacrylamide-acrylamide random copolymer again, and add it to the bottle,

[0038] 6. Gently shake the bottle and mix all the ingredients, then centrifuge at 1000rpm for 15min, all the contents will be at the bottom of the ...

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Abstract

The invention discloses a blended copolymer gel with a capillary self-coating effect and a preparation method thereof. According to the preparation method, the blended copolymer gel is obtained by mixing a self-coating polymer with a screening polymer which polymerizes self-coating polymer monomers in a sol buffer. The blended copolymer gel prepared with the preparation method has a good separation capability and a capillary inner wall self-coating capability in the process of capillary electrophoresis, so the adsorption of the capillary inner wall to DNA molecules is effectively reduced, andthe service life of the capillary is increased.

Description

technical field [0001] The invention relates to a blended copolymer gel with capillary self-coating function and a preparation method thereof. Background technique [0002] Capillary electrophoresis is a fast and efficient separation method. Charged species move from one end of the capillary to the other under the action of an electric field. [0003] Capillary gel electrophoresis is one of the most effective methods for separating macromolecules, such as biological macromolecules RNA and DNA, peptides, proteins, etc. Capillary gel electrophoresis usually uses quartz capillaries with an inner diameter ranging from 50 microns to 200 microns and a length from 10 cm to 100 cm. In order to protect the analyte from being adsorbed and eliminate the electroosmotic flow of the capillary, the inner wall of the capillary is usually coated with a protective layer. The most commonly used coating is polyacrylamide coating. The properties of the separation medium of capillary gel elec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/075C08L33/26G01N27/453
Inventor 赵兴春叶健姜伯玮
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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