Method for evaluating the effect of penehyclidine hydrochloride on the m-receptor of dysmenorrhea animal model cells
A technology of penehyclidine hydrochloride and animal models, which is applied in the direction of testing pharmaceutical preparations, material inspection products, etc., and can solve problems such as the mechanism of dysmenorrhea treatment that has not been fully understood
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Embodiment 1
[0019] Adult rats were taken and divided into random groups; one group was intraperitoneally injected with estradiol 1 mg / kg, once a day, for three consecutive days, and then their uteri were taken, and 0.001 unit of oxytocin was added to a constant temperature water bath for 10 minutes to obtain animal The model uterus; the other group was the blank control group.
[0020] Animal model uteri were taken and randomly divided into three groups, one group was the model group with successful dysmenorrhea model modeling, and one group was the positive control group. -5 mol / L, the final concentration of atropine after adding was 0.8mg / ml and acted for 5min, one of which was the experimental group adding 10 -7 mol / L penhyclidine hydrochloride to its final concentration of 0.0067mg / ml for 5min.
[0021] The uteri of the blank control group, the model group, the positive control group and the experimental group were pulverized and homogenized, and the interstitial fluid was centrifuge...
Embodiment 2
[0023] Adult rats were taken and randomly divided into groups; one group was intraperitoneally injected with estradiol 1mg / kg, once a day, for three consecutive days, and then their uteri were removed, and 0.001 unit of oxytocin was added to a constant temperature water bath at 37°C for 15 minutes. The animal model uterus was obtained; the other group was a blank control group.
[0024] Animal model uteri were taken and randomly divided into three groups, one group was the model group with successful dysmenorrhea model modeling, and one group was the positive control group. -5 mol / L, the final concentration of atropine after adding was 0.8mg / ml and acted for 10min, one of them was the experimental group adding 5×10 -7 mol / L of penhyclidine hydrochloride to a final concentration of penhyclidine hydrochloride of 0.013 mg / ml for 10 minutes.
[0025] The uteri of the blank control group, the model group, the positive control group and the experimental group were pulverized and ho...
Embodiment 3
[0027] Adult rats were taken and randomly divided into groups; one group was intraperitoneally injected with estradiol 1mg / kg, once a day, for three consecutive days, and then their uteri were removed, and 0.001 unit of oxytocin was added in a constant temperature water bath at 37°C for 20 minutes. The animal model uterus was obtained; the other group was a blank control group.
[0028] Animal model uteri were taken and randomly divided into three groups, one group was the model group with successful dysmenorrhea model modeling, and one group was the positive control group. -5 mol / L, the final concentration of atropine after adding was 0.8mg / ml and acted for 15min, one of them was the experimental group adding 20×10 -7 mol / L penhyclidine hydrochloride to its final concentration of 0.02mg / ml for 15min.
[0029] The uteri of the blank control group, the model group, the positive control group and the experimental group were pulverized and homogenized, and the interstitial fluid...
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