Two endophytic fungus strains for promoting Lilium davidii var. unicolor growth and resistance to drought stress

A technique for promoting plant growth under drought stress, applied in the field of endophytic fungal strains, to achieve the effects of solving water shortage, increasing tolerance, and promoting rapid recovery and growth

Inactive Publication Date: 2015-01-14
北京市辐射中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These two strains of fungi were preserved in the General Microorganism Center of the China Committee for the Collection of Microbial Cultures on May 30, 2013

Method used

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  • Two endophytic fungus strains for promoting Lilium davidii var. unicolor growth and resistance to drought stress
  • Two endophytic fungus strains for promoting Lilium davidii var. unicolor growth and resistance to drought stress
  • Two endophytic fungus strains for promoting Lilium davidii var. unicolor growth and resistance to drought stress

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Isolation and purification of endophytic fungi:

[0023] Rinse the roots of the bulbs of Lanzhou lily collected from Lanzhou and Shandan bulbs collected from Baihuashan in Beijing with tap water, then rinse with distilled water, and then perform surface disinfection after drying: treat with 70% alcohol for 40 seconds, rinse with sterile water, and then use 0.1% mercuric chloride Treat for 7 minutes, rinse with sterile water 3-4 times. Under sterile conditions, take 100uL of the sterile water produced by the last rinse and spread it on the PDA medium, incubate at 25°C for 24-72 hours, observe whether there are colonies, and verify whether this disinfection method has killed the bacteria. Microorganisms on the surface of the test material.

[0024] Cut the basal roots into 0.2 cm long root segments under aseptic conditions, place them in PDA medium, and culture them statically at 25°C. After the hyphae appear in the tissue block, pick the hyphae and transfer them to a n...

Embodiment 2

[0026] Growth-promoting effect of LZ1 on tissue-cultured seedlings of Lilium lanzhou

[0027] A glass petri dish with a diameter of 12cm contains 80mL MS+20g / L sucrose+agar 7%, and inoculates 2 Lanzhou lily tissue culture seedlings in each dish; the LZ1 strain activated on the PDA medium is punched with a 0.5mm hole punch Bacteria sheet, one bacterium sheet was inoculated at a distance of 1 cm from the seedling, and a piece of PDA medium of the same size was inoculated as a control. Cultivate under the conditions of day / night=25°C / 18°C, photoperiod 14 / 10h, and illuminance 2000Lx. After co-cultivation for 35 days, the growth status of the Lanzhou lily tissue culture seedlings in the inoculation group was significantly better than that in the control group ( figure 1 ), the plant height and seedling dry weight of the seedlings co-cultured with the fungus LZ1 increased by 35.8% and 57.1%, respectively, and the number of leaves and root branches increased by 95.5% and 52.9% compa...

Embodiment 3

[0029] Growth-promoting effect of LZ1 on Lilium lanzhou seedlings under drought stress

[0030] Spread a layer of poplar (Salicaceae Salicaceae) leaves crushed to a size of 0.5-1 cm on the base of a 250 mL Erlenmeyer flask, then put 50 g of vermiculite into it, and sterilize at 121° C. for 1 h. Add 25mL of MS+20g / L sucrose nutrient solution to each bottle, place 0.4mL of the bacteria cultured on the PDA liquid medium on the leaves in a sterile state, inoculate the Lanzhou lily tissue culture seedlings on the leaves, and inoculate the roots with vermiculite. Buried well, the control group without inoculation was inoculated with the same volume of PDA liquid medium. 2 seedlings were inoculated in every bottle, sealed with a parafilm with air holes, and cultivated under continuous light at 28°C and an illumination of 2000Lx. At the beginning of the experiment, the relative water content of the matrix reached about 90%, and then placed in the culture room to make it dry naturally...

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Abstract

The invention discloses two endophytic fungus strains for promoting Lilium davidii var. unicolor growth and resistance to drought stress. Drought stress is an important reason for inhibiting high-efficiency production of Lilium davidii var. unicolor. The two endophytic fungus strains have a very important meaning for promoting Lilium davidii var. unicolor resistance to drought stress. The two endophytic fungus strains can be colonized at the root of Lilium davidii var. unicolor, is used for Lilium davidii var. unicolor seedling growing or cultivation and can effectively promoting Lilium davidii var. unicolor growth and resistance to drought stress.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to two strains of endophytic fungi that promote the growth of Lanzhou lily and are resistant to drought stress. These two fungi are used in the seedling cultivation or cultivation of Lanzhou lily, which can effectively promote the growth of lily and resist drought stress. Background technique [0002] Lanzhou lily (Lilium davidii var. unicolor) is a variety of Sichuan lily, which has been cultivated in Lanzhou, Gansu and its surrounding areas for more than 100 years. Lanzhou lily bulbs are plump and white, soft in texture, sweet and sweet, and are treasures among lilies; at the same time, Lanzhou lily also contains various medicinal ingredients such as colchicine, lilyside, vitamins, mineral elements, amino acids, etc. Qi, regulating the spleen and invigorating the stomach, calming the mind and calming the nerves, nourishing the lungs and relieving cough, clearing heat and d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P21/00C12R1/645
CPCA01N63/30C12N1/145C12R2001/645
Inventor 武利勤尚宏忠白锦荣顾海科王青刘桂君
Owner 北京市辐射中心
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