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A method for extracting oil from oil-producing microorganisms

A technology for oil-producing microorganisms and oils, which is applied in the production of fat, fat oil/fat, etc., can solve the problems of high energy consumption and low oil extraction rate, and achieve the effect of improving oil extraction rate and reducing energy consumption.

Active Publication Date: 2017-07-25
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the defects of low oil extraction rate and high energy consumption in the prior art of extracting oil from oleaginous microorganisms, and provide an efficient and economical method for extracting oil from oleaginous microorganisms

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] This example is used to illustrate the method for extracting fat from oil-producing microorganisms of the present invention.

[0049] (1) Weigh 45 g of 11 wt% water slurry containing chlorella and place it in a mechanically stirred reactor. Add 675 g of ferric chloride. Under hydrothermal conditions at a temperature of 90°C, relative to each cubic meter of water The stirring power of the materials to be stirred is 0.6kW (using the YXMD analog booster electric stirrer of Shandong Juancheng Yongxing Instrument Factory, the same below), stir for 40 minutes to obtain the chlorella soluble;

[0050] (2) Centrifuge the chlorella lysate at 5000 rpm for 5 minutes, and collect the upper oil phase to obtain 0.76 g of oil. The oil extraction rate is calculated to be 15.4%.

Embodiment 2

[0052] This example is used to illustrate the method for extracting fat from oil-producing microorganisms of the present invention.

[0053] (1) Weigh 35g of 14% by weight aqueous slurry containing chlorella, place it in a mechanically stirred reactor, add 700g of aluminum sulfate, and under hydrothermal conditions at a temperature of 80°C, relative to each cubic meter of water that is stirred Material, the stirring power is 0.2kW, stirring for 60 minutes, to obtain the chlorella lysate;

[0054] (2) Centrifuge the chlorella soluble slurry at 6000 rpm for 4 minutes, collect the upper oil phase to obtain 0.69 g of oil, and calculate the oil extraction rate to be 14.1%.

Embodiment 3

[0056] This example is used to illustrate the method for extracting fat from oil-producing microorganisms of the present invention.

[0057] (1) Weigh 55 g of 9 wt% water slurry containing chlorella and place it in a mechanically stirred reactor. Add 440 g of magnesium chloride. Under hydrothermal conditions at a temperature of 100°C, relative to each cubic meter of material being stirred , The stirring power is 0.3kW, stirring for 30 minutes, to obtain the chlorella lysate;

[0058] (2) Centrifuge the chlorella lysate at 4000 rpm for 6 minutes, collect the upper oil phase to obtain 0.54 g of oil, and calculate the oil extraction rate to be 10.9%.

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Abstract

The invention discloses a method for extracting oil from oil production microbes. The method is characterized in that the method comprises the following steps: 1, allowing a water slurry containing the oil production microbes to contact with an acidic metal salt or its aqueous solution, and stirring under hydrothermal conditions to dissolve out oil in the cells of the oil production microbes in order to obtain an oil production microbes dissolved slurry containing oil; and 2, separating the oil from the oil production microbes dissolved slurry. The method for extracting oil from oil production microbes allows the water slurry containing the oil production microbes to contact with the acidic metal salt or its aqueous solution, and stirring to be carried out under low-temperature hydrothermal conditions in order to dissolve out oil in the cells of the oil production microbes, and the water phase and the oil phase of the oil production microbes dissolved slurry can be easily separated, so the energy consumption is greatly reduced, and the oil extraction ate is improved.

Description

Technical field [0001] The invention relates to the technical field of oil-producing microorganism engineering, in particular to a method for extracting oil from oil-producing microorganisms. Background technique [0002] Due to the rapid proliferation of microbial cells, short production cycle, wide sources of raw materials for microbial growth, low prices, and the growth of microbes is not restricted by seasons and climate changes, continuous large-scale production, low production costs, and high oil content in oil-producing microorganisms Therefore, the use of oil-producing microorganisms to produce oil has become an important means to alleviate the global energy crisis. [0003] Microalgae is a kind of aquatic photosynthetic autotrophic microorganisms. It uses light energy to CO 2 And H 2 O is used as a substrate to grow cells and synthesize large amounts of triglycerides (TAGs), polysaccharides, proteins and other substances in the cells. Among them, triglycerides (TAGs) incl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C11B1/00C11B1/14C11B1/10
CPCC11B1/10C11B1/14
Inventor 于鹏荣峻峰林伟国周旭华纪洪波
Owner CHINA PETROLEUM & CHEM CORP