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Method for extracting oil from oil production microbes

A technology for oil-producing microorganisms and oil extraction, which is applied in the direction of fat production, fat oil/fat production, etc., can solve the problems of high energy consumption and low oil extraction rate, and achieve the effect of increasing oil extraction rate and reducing energy consumption

Active Publication Date: 2015-01-21
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the defects of low oil extraction rate and high energy consumption in the prior art of extracting oil from oleaginous microorganisms, and provide an efficient and economical method for extracting oil from oleaginous microorganisms

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] This example is used to illustrate the method for extracting oil from oleaginous microorganisms of the present invention.

[0049] (1) Weigh 45g of 11% by weight water slurry containing chlorella, place it in a mechanically stirred reaction kettle, add 675g of ferric chloride, and under the hydrothermal condition with a temperature of 90°C, the Stirring the material, the stirring power is 0.6kW (using the YXMD analog booster electric stirrer of Juancheng Yongxing Instrument Factory, Shandong Province, the same below), and stirring for 40 minutes to obtain the chlorella slurry;

[0050] (2) The chlorella slurry was centrifuged at 5000rpm for 5 minutes, and the upper oil phase was collected to obtain 0.76g of oil. The calculated oil extraction rate was 15.4%.

Embodiment 2

[0052] This example is used to illustrate the method for extracting oil from oleaginous microorganisms of the present invention.

[0053] (1) Weigh 35g of 14% by weight water slurry containing chlorella, place it in a mechanically stirred reactor, add 700g of aluminum sulfate, and under hydrothermal conditions at a temperature of 80°C, the material, stirring power is 0.2kW, stirring for 60 minutes to obtain chlorella slurry;

[0054] (2) The chlorella slurry was centrifuged at 6000rpm for 4 minutes, and the upper oil phase was collected to obtain 0.69g of oil, and the calculated oil extraction rate was 14.1%.

Embodiment 3

[0056] This example is used to illustrate the method for extracting oil from oleaginous microorganisms of the present invention.

[0057] (1) Weigh 55g of 9% by weight water slurry containing chlorella, put it in a mechanically stirred reactor, add 440g of magnesium chloride, and under the hydrothermal condition with a temperature of 100°C, the amount of material being stirred is , the stirring power is 0.3kW, stirring for 30 minutes to obtain the chlorella slurry;

[0058] (2) The chlorella slurry was centrifuged at 4000rpm for 6 minutes, and the upper oil phase was collected to obtain 0.54g of oil, and the calculated oil extraction rate was 10.9%.

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PUM

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Abstract

The invention discloses a method for extracting oil from oil production microbes. The method is characterized in that the method comprises the following steps: 1, allowing a water slurry containing the oil production microbes to contact with an acidic metal salt or its aqueous solution, and stirring under hydrothermal conditions to dissolve out oil in the cells of the oil production microbes in order to obtain an oil production microbes dissolved slurry containing oil; and 2, separating the oil from the oil production microbes dissolved slurry. The method for extracting oil from oil production microbes allows the water slurry containing the oil production microbes to contact with the acidic metal salt or its aqueous solution, and stirring to be carried out under low-temperature hydrothermal conditions in order to dissolve out oil in the cells of the oil production microbes, and the water phase and the oil phase of the oil production microbes dissolved slurry can be easily separated, so the energy consumption is greatly reduced, and the oil extraction ate is improved.

Description

technical field [0001] The invention relates to the technical field of oleaginous microorganism engineering, in particular to a method for extracting oil from oleaginous microorganisms. Background technique [0002] Due to the rapid proliferation of microbial cells, short production cycle, wide sources of raw materials required for microbial growth, low price, and microbial growth is not limited by seasons and climate changes, continuous large-scale production is possible, production costs are low, and the oil content of oil-producing microorganisms is high. , therefore, the use of oleaginous microorganisms to produce oil has become an important means to alleviate the global energy crisis. [0003] Microalgae are aquatic photoautotrophic microorganisms that use light energy to produce CO 2 and H 2 O acts as a substrate for cell growth and synthesizes a large amount of triglycerides (TAGs), polysaccharides, proteins and other substances in cells. Among them, triglycerides ...

Claims

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Application Information

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IPC IPC(8): C11B1/00C11B1/14C11B1/10
CPCC11B1/10C11B1/14
Inventor 于鹏荣峻峰林伟国周旭华纪洪波
Owner CHINA PETROLEUM & CHEM CORP