Method for Improving the Accuracy of Determination of Molecular Weight of Water-Soluble Polymer by Gel Chromatography
A water-soluble polymer, gel chromatography technology, applied in the field of water treatment, can solve problems such as inability to obtain, interfere with inverted peaks, and continuous peaks, and achieve the effects of improving accuracy, avoiding interference and inverted peaks, and simple operation steps.
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Embodiment 1
[0022] Take 2g of commercially available polyacrylic acid (PAA), adjust its pH to 7.5 with 10mol / L NaOH solution, and convert PAA into salt; take 50mL of methanol in a beaker, and drop the sample converted into salt into methanol, while Stir, the precipitated solid is the sample to be tested; let it stand for 10 minutes and pour out the methanol, add new methanol to the precipitate and stir and wash again, repeat three times; put the precipitate in a vacuum drying oven at 70°C to dry to constant weight, Cool for later use; determine the molecular weight and its distribution using gel chromatography.
[0023] The molecular weight and molecular weight distribution results of PAA are shown in Table 1 before and after using the method of the present invention, and the spectrogram is shown in figure 1 and figure 2 . As can be seen from the spectrograms, the PAA spectrograms processed by the method of the present invention are significantly improved, the baseline is stable, the p...
Embodiment 2
[0027] Take 2g of commercially available AA-MA copolymer, adjust its pH to 8 with 10mol / L NaOH solution, and convert the polymer into salt; take 50mL of methanol in a beaker, and drop the sample converted into salt into methanol, Stir at the same time, the precipitated solid is the sample to be tested; let it stand for 10 minutes and pour out the methanol, add new methanol to the precipitate and stir and wash again, repeat three times; put the precipitate in a 70°C vacuum drying oven and dry to constant weight , cooled for later use; use gel chromatography to determine the molecular weight and its distribution.
[0028] The molecular weight and molecular weight distribution results of the AA-MA copolymer are shown in Table 2 before and after using the method of the present invention, and the spectrogram is shown in image 3 and Figure 4 . As can be seen from the spectrogram, the AA-MA copolymer processed by the method of the present invention is significantly improved, the ...
Embodiment 3
[0032] Take 2g of commercially available AA-AMPS multi-polymer copolymer, adjust its pH to 9 with 10mol / L NaOH solution, and convert the polymer into salt; take 50mL of methanol in a beaker, and drop the sample converted into salt into methanol drop by drop , stirring at the same time, the precipitated solid is the sample to be tested; let stand for 10 minutes and pour out the methanol, add new methanol to the precipitate and stir and wash again, repeat three times; put the precipitate in a vacuum drying oven at 70°C and dry until constant Weight, cooled for later use; use gel chromatography to determine the molecular weight and its distribution.
[0033] The results of molecular weight and molecular weight distribution of AA-AMPS multi-polymer copolymer before and after using the method of the present invention are shown in Table 3, and the spectrogram is shown in Figure 5 and Figure 6 . As can be seen from the spectrogram, the AA-AMPS multi-component copolymer processed ...
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