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Construction method and application of Zihai hybrid scallop restorer line

A method for constructing a restorer line, which is applied in the field of shellfish breeding technology--hybrid scallop commercial seedling cultivation, can solve the problems of inability to establish male sterile lines, maintainer lines and restorer lines, and achieves overcoming obstacles to self-fertilization, low cost effect

Active Publication Date: 2016-02-10
烟台海之春水产种业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, in animal breeding, there have been no reports of male sterile and female fertile individuals at home and abroad so far, so it is impossible to establish male sterile lines, maintainer lines and restorer lines similar to crops for hybrid breeding

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1) Breeding of Zihai hybrid scallop restorer lines: Select 50 large individual scallops from the male sterile line of Zihai hybrid scallops, fertilize them with sperm from different bay scallops after promoting maturation and oviposition, and establish at least 20 backcross families At the same time, the Gulf scallops for sperm were self-propagated to establish an inbred family, and the growth and survival of the backcross progeny were measured, and the combining ability test was carried out; from the second year onwards, according to the results of the combining ability test and the development of the gonads, select among them with excellent traits and gonad development. For the backcross family containing hermaphroditic scallops, the shellfish of the related male sterile family will be continuously backcrossed with the sperm of the Gulf scallop hybrid family for sperm to establish a backcross family until all the backcross families are hermaphroditic and have good trait...

Embodiment 2

[0024]At the end of March 2008, purple scallops with mature gonads were taken, and purple scallop eggs were obtained after temperature stimulation. After insemination with the sperm of bay scallops, the first-generation seedlings of purple scallops and bay scallops were obtained and raised. In the spring of 2009, the first-generation scallops of the above-mentioned hybrids were matured, and the scallops that were large and only developed in the female area were selected from them. After drying in the shade for 30 minutes, the scallops were placed in a container filled with seawater at 23°C to stimulate spawning. The sperm of scallops were mixed and fertilized, and a backcross population of Zihai hybrid scallops and bay scallops was bred. Most of the backcross populations were hermaphroditic after maturation in the spring of 2010, but <10% of the individuals were still male sterile.

[0025] Continue to backcross the above-mentioned male sterile individuals with the sperm of th...

Embodiment 3

[0029] In the spring of 2010, male sterile individuals from the first generation of hybrids of purple scallop eggs and bay scallop sperm established in 2009 were selected, stimulated to spawn after maturation, and fertilized with bay scallop sperm. 12 backcross families were established and raised. become. After maturation in the spring of 2011, it was found that most of the individuals in 11 of the families were hermaphroditic, while most of the individuals in one of the families only developed the female region, and a small number of individuals were agnostic.

[0030] According to this method, the eggs of a single male sterile individual were backcrossed with the sperm of a single bay scallop to establish several backcross families, and the sea scallops that provided sperm were self-crossed to establish corresponding self-bred families. In the second year, check the gonad development of the backcross family. If the backcross family is all hermaphroditic scallops, only one g...

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PUM

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Abstract

The invention relates to a construction method and application of an argopecten purpuratus and argopecten irradians irradians hybridized scallop restoring line. The restoring line belongs to an argopecten irradians irradians strain which enables male sterile line progenies to restore the male fertile property, wherein argopecten purpuratus and argopecten irradians irradians hybridized scallop belongs to hybrid progenies of argopecten purpuratus ovums and argopecten irradians irradians sperms. The construction method comprises the steps that excellent individuals in the male sterile line are selected, which are continuously backcrossed through different argopecten irradians irradians until all progenies are hermaphrodite, then the restoring line of the male sterile line is adopted, and finally the restoring line and corresponding male sterile line are subjected to hybridization, so that argopecten purpuratus and argopecten irradians irradians hybridized scallop product seedlings are produced. The method establishes a male sterile line restoring line in animals for the first time, and effectively overcomes the obstacle of autofertilization during hermaphrodite scallop hybridization, and the annual yield of the produced argopecten purpuratus and argopecten irradians irradians hybridized scallops is increased by 40 percent above compared with that of argopecten irradians irradians.

Description

technical field [0001] The invention belongs to shellfish breeding technology-a method for cultivating commercial hybrid scallop seedlings, and in particular relates to a construction method and application of a restorer line of Zihai hybrid scallop (the hybrid offspring of purple scallop ova and bay scallop sperm). Background technique [0002] Bay scallops (Argopecten irradians sirradians) are native to the Atlantic coast of the United States and were introduced into my country in 1982. The bay scallop grows fast, has a wide range of temperature adaptability, and has strong stress resistance. It is very suitable for farming in the northern seas of my country. At present, the output and farming area account for more than 50% of the scallop farming in my country. However, due to the long-term inbreeding after the introduction, the germplasm of bay scallops has been seriously degraded during the breeding process. The size of commercial shellfish is getting smaller and smaller...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 王春德孙奎府马斌
Owner 烟台海之春水产种业科技有限公司
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