A method for extracting Kudingcha saponins from Kudingcha

Abstract

The invention discloses a method for extracting broadleaf holly leaf saponin from broadleaf holly leaves. The method comprises the following steps: treating a raw material, performing high-pressure micro jet flow superfine grinding treatment, extracting at high pressure, collecting the extracted liquid, centrifuging, performing AB-8 resin column chromatography on the supernate, eluting by using a 50-60% ethanol solution, concentrating, and performing spray-drying, thereby obtaining broadleaf holly leaf saponin. The method has the advantages of high content, high extraction rate, simple process, production application, low production cost and the like.

Description

technical field [0001] The invention relates to an extraction method in the field of biotechnology, in particular to a method for extracting kudingcha saponins from kudingcha. Background technique [0002] Kudingcha (Ilexkudingcha C.J.Tseng), English name: Leaf of Chinese Holly, leaf of Broadleaf Holly, is an evergreen tree of the genus Kudingcha in the Ilex family Ilex, commonly known as Chading, Fudingcha, Gaolu tea, mainly distributed in Southwest China (Sichuan Province) , Chongqing, Guizhou, Hunan, Hubei) and South China (Jiangxi, Yunnan, Guangdong, Fujian, Hainan) and other places, it is a traditional pure natural health drink in my country. Kuding tea contains more than 200 ingredients such as Kuding saponins, amino acids, vitamin C, polyphenols, flavonoids, caffeine, and proteins. The finished tea has a bitter taste and a sweet and cool taste. It has many functions such as clearing away heat and relieving heat, improving eyesight and improving intelligence, promoting...

AI Technical Summary

Problems solved by technology

But there are some defects in these methods, for example: the solvent extraction method takes a long time and is prone to emulsification layer, which seriously affects the extraction effect and purity of total glycosides. This method is not suitable for the processing of a large number of samples; macroporous resin adsorption method: the oil in plants, Lipophilic components or low-polar components are easy to combine with macroporous resins, coating a layer of grease on the surface of the resin, and when the solvent of the macroporous resin is eluted into water or a hydrophilic solvent, it will greatly reduce the viscosity of the macroporous resin. The adsorption capacity seriously affects the separation effect; the solvent precipitation method needs to add a high amount of organic solvent, the amount of organic solvent is large, it pollutes the environment, and it is difficult to concentrate, the purity of the extract is also low, and there are still problems of organic solvent residue; cholesterol Precipitation method: the molecular complex formed by Kubuchaglucoside and sterol is not stable, so it is not suitable for purification by cholesterol precipitation method; lead salt precipitation method: the process is more complicated, the process of removing lead is difficult, the content of heavy metal lead is high, and the color of the product is very dark , it is difficult to meet the requirements of food and drug standards; Chromatography: This method is similar to the resin method, and also has the same defects as the macroporous resin adsorption method. In addition, the particle size of alumina used for chromatography is too large and the separation effect is poor, and the particle size is too small Solvent flow rate is slow, easy to spread the band

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