Separation method of polyphenol compounds in highland barley dietary fiber
A technology of polyphenolic compounds and dietary fiber, which is applied in the field of separation of polyphenolic compounds from highland barley dietary fiber, can solve the problems of no extraction of polyphenolic compounds and low actual content of polyphenolic compounds, and achieve the effect of simple operation
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Embodiment 1
[0022] 1) The highland barley flour is degreased repeatedly 3 times with ether at a ratio of mass to volume 1:20, each time for 10 minutes;
[0023] 2) Accurately weigh 2 g of defatted highland barley flour into a centrifuge tube, add 40 mL of 70% (v / v) acetone, extract on a shaking table in the dark for 2 h at room temperature, centrifuge at 4000 / min for 10 min to obtain a supernatant, repeat 3 times, and combine The supernatant liquid and the precipitated residue are recovered for later use;
[0024] 3) The supernatant obtained in step (2) was vacuum rotary evaporated at 30°C to about 50mL, adjusted to pH 2 with 6mol / L hydrochloric acid, extracted 5 times with diethyl ether according to the extraction solution 1:1 (v / v), and the aqueous phase Recovered for later use, the organic phase was evaporated to dryness in vacuum at 30°C, and dissolved in methanol to obtain 526.80 μg of free polyphenolic compound;
[0025] 4) The aqueous phase that step (3) obtains is filled with N w...
Embodiment 2
[0029] 1) Weigh the sample and number it, and place the sample in a 2501mL conical flask:
[0030] Table 1
[0031] Sample type quality Numbering Yunhei Highland Barley Flour 2.0093g 1 lyophilized SDF 2.0015g 2 lyophilized IDF 2.0181g 3
[0032] 2) Add 40mL of 70% (v / v) acetone respectively, extract on shaker in the dark at room temperature for 2h, centrifuge at 4000 / min for 10min to obtain the supernatant, repeat 3 times, combine the supernatant, and use the precipitated residue to extract the insoluble bonded polyphenolic compounds;
[0033] 3) The supernatant obtained in step (2) was vacuum rotary evaporated at 30°C to about 50mL, adjusted to pH 2 with 6mol / L hydrochloric acid, extracted 5 times with diethyl ether according to the extraction solution 1:1 (v / v), and the aqueous phase It is used to extract esterified polyphenol compounds. The supernatant of No. 3 sample was not separated for 9 minutes during the third extraction, and it...
Embodiment 3
[0045] 1) Weigh the sample and number it, and place the sample in a 2501mL conical flask:
[0046] Table 4
[0047]
[0048] 2) Add 40mL of 70% (v / v) acetone respectively, extract on a shaker in the dark at room temperature for 2 hours, centrifuge at 4000 / min for 10 minutes to obtain the supernatant, repeat 3 times, combine the supernatant, and store the precipitated residue under nitrogen gas for use Extraction of insoluble bonded polyphenolic compounds;
[0049] 3) The supernatant obtained in step (2) was vacuum rotary evaporated at 30°C to about 50 mL, adjusted to pH 2 with 6 mol / L hydrochloric acid, extracted 5 times with diethyl ether according to the extraction solution 1:1 (v / v), fixed each The extraction time is 5 minutes. The aqueous phase is used to extract the esterified polyphenolic compounds. The organic phase is evaporated to dryness under vacuum at 30°C, and the free polyphenolic compounds are dissolved in methanol. The raw material obtained 38.10 μg, and t...
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