Detection, isolation and analysis of rare cells in biological fluids
A technology for rare cells and biological liquids, which is used in the analysis of biological materials, the determination/inspection of microorganisms, biological testing, etc.
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[0068] Cell-free 4B9ELISA
[0069] The 4B9 ELISA in one embodiment of the present invention comprises coating the 4B9 antibody directly or indirectly on a solid support, and using horseradish peroxisome (HRPO)-labeled goat anti-mouse IgM (Fab) 2 to detect bound 4B9, and the reaction was quantified colorimetrically using the HRPO substrate tetramethylbenzidine (TMB). However, in direct coating, by incubation with detection anti-mouse antibody-HRPO conjugate (0.025ug / ml assay buffer; 10mM NaPO 4 , pH 7.4, containing 8.8g NaCl, 0.5g BSA, 0.5mL Tween-20, and 2.5mL biopreservative proclin / L) to detect 4B9, while indirect coating involves unlabeled or biotinylated 4B9 (10 ug / mL) were pre-incubated with secondary antibodies or streptavidin-coated supports, respectively.
[0070] Typically, microtiter wells, magnetic particles in test tubes, and 16 Slides in a well partitioning kit (Grace Bio labs) were used for comparative evaluation. After four washes with ELISA wash buffer (0.0...
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