Detection, isolation and analysis of rare cells in biological fluids

A technology for rare cells and biological liquids, which is used in the analysis of biological materials, the determination/inspection of microorganisms, biological testing, etc.

Inactive Publication Date: 2015-02-18
KELLBENX
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention also addresses the unmet need for reliable rare cell isolation methods in other fields that currently face similar detection and analysis limitations, such as circulating stem cells and tumor cells

Method used

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  • Detection, isolation and analysis of rare cells in biological fluids
  • Detection, isolation and analysis of rare cells in biological fluids
  • Detection, isolation and analysis of rare cells in biological fluids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0068] Cell-free 4B9ELISA

[0069] The 4B9 ELISA in one embodiment of the present invention comprises coating the 4B9 antibody directly or indirectly on a solid support, and using horseradish peroxisome (HRPO)-labeled goat anti-mouse IgM (Fab) 2 to detect bound 4B9, and the reaction was quantified colorimetrically using the HRPO substrate tetramethylbenzidine (TMB). However, in direct coating, by incubation with detection anti-mouse antibody-HRPO conjugate (0.025ug / ml assay buffer; 10mM NaPO 4 , pH 7.4, containing 8.8g NaCl, 0.5g BSA, 0.5mL Tween-20, and 2.5mL biopreservative proclin / L) to detect 4B9, while indirect coating involves unlabeled or biotinylated 4B9 (10 ug / mL) were pre-incubated with secondary antibodies or streptavidin-coated supports, respectively.

[0070] Typically, microtiter wells, magnetic particles in test tubes, and 16 Slides in a well partitioning kit (Grace Bio labs) were used for comparative evaluation. After four washes with ELISA wash buffer (0.0...

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Abstract

The invention provides a method for isolating or enriching a rare cell from a biological fluid of a mammal employing an antibody that binds a cell-surface antigen of the rare cell. The immobilized antibody is incubated with a sample of biological fluid that includes the rare cells and a plurality of other cells so as to form an antibody-rare cell complex. The complex can be detected or isolated and subsequently analyzed by any of a variety of physical, chemical and genetic techniques.

Description

technical field [0001] The present invention relates to immunological methods and kits for the detection, capture and isolation of rare cells from biological fluids for analysis of their antigenic, phenotypic and genetic properties. In particular, the present invention provides methods for the detection, capture, isolation and analysis of fetal mononuclear red blood cells (NRBC) in maternal blood. Background technique [0002] Performing prenatal diagnosis to detect possible chromosomal and genetic abnormalities in the fetus enables parents and caregivers to initiate early treatment for monitoring a predisposition to a disease or condition. The practice of prenatal diagnosis has been established to detect possible chromosomal and genetic abnormalities in the fetus, thus enabling parents and caregivers to make informed decisions. Down syndrome (DS) due to the presence of all or part of an extra copy of ), the most common genetic cause of mental retardation and the leading r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/68
CPCC12Q1/6879G01N33/56966G01N33/57492C12Q1/6806C12Q2563/131C12Q2563/149G01N33/80G01N2333/805
Inventor J·科霍斯罗维L·H·凯尔纳H·班娜妮
Owner KELLBENX
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