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Chinese wampee fruit extract and application thereof in preparation of medicine for preventing and treating alzheimer disease

A technology of extract and peel fruit, applied in the field of wampee fruit extract and its application in the preparation of drugs for the prevention and treatment of Alzheimer's disease, to achieve good results and relieve cognitive dysfunction

Active Publication Date: 2015-03-04
GUANGDONG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no evidence that huangpi fruit extract can prevent or treat cognitive dysfunction caused by AD, and there is no related report that huangpi fruit extract can inhibit the generation of Aβ and reduce the resulting neuronal apoptosis.

Method used

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  • Chinese wampee fruit extract and application thereof in preparation of medicine for preventing and treating alzheimer disease
  • Chinese wampee fruit extract and application thereof in preparation of medicine for preventing and treating alzheimer disease
  • Chinese wampee fruit extract and application thereof in preparation of medicine for preventing and treating alzheimer disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The preparation of embodiment 1 Huangpi fruit extract

[0042] Take 5.00kg of dried wampee fruit, remove the core, crush, pass through a 40-mesh sieve, and set aside. Add 9 times the weight of medicinal materials in 95% ethanol to soak for 12-14 hours, ultrasonically extract 5 times, the time of each ultrasonic extraction is 1.5h, filter, combine the filtrate, centrifuge at 3000rpm / min for 10min, take the supernatant and concentrate to a relative density of 1.20- 1.45, extracted 3 times with an equal volume of petroleum ether, separated the upper layer extract (petroleum ether extraction part), evaporated the petroleum ether from the lower layer liquid, then extracted 5 times with an equal volume of ethyl acetate, combined the upper layer ethyl acetate extracts and the lower layer extract, concentrated under reduced pressure, and dried to obtain 74.60 g of ethyl acetate extract and 160.70 g of the lower layer extract. Dilute the obtained ethyl acetate extract and lower...

Embodiment 2 Embodiment 1

[0044] Embodiment 2 is to the qualitative identification of the physical and chemical characteristics and chemical structure of 5 kinds of compounds obtained in embodiment 1

[0045] Compound 1: Rutin

[0046] Yellow solid powder (methanol), with ultraviolet absorption at 254nm, vanillin sulfate solution is pale yellow. ESI-MS (positive) gave a quasi-molecular ion peak m / z 633[M+Na]+ suggesting a molecular weight of 610. Combined with 1H and 13C NMR, it is deduced that the molecular formula is C 27 h 30 o 16 , with a calculated unsaturation of 12.

[0047] In 1H NMR (400MHz, DMSO-d6), δH: 3.05-5.14 (sugar protons), 0.99 (3H, d, J = 6.4Hz, H-6''), 5.27 (1H, brs, H-1" '),5.34(1H,d,J=7.6Hz,H-1"),6.19(1H,d,J=2.0Hz,H-6),6.39(1H,d,J=1.6Hz,H-8 ), 6.84(1H,d,J=7.6Hz,H-5'),7.54(2H,d,J=10.0Hz,H-2',6').13C-NMR(DMSO-d6,100MHz)δC :177.4(C-4),164.2(C-7),161.2(C-5),156.4(C-9),156.4(C-2),148.5(C-3'),144.8(C-4' ),133.5(C-3),121.6(C-6'),121.2(C-1'),116.0(C-5'),115.1(C-2'),104.0(C-10),101.2...

Embodiment 3

[0060] The composition ratio range analysis of the compound in the example 3 Huangpi fruit extract

[0061] HPLC was used to detect the extract of Pseudomonas japonica, liquid phase system: Agilent 1200, chromatographic column: Thermo Syncronis RP-C18 (250×4.6mm, 5μm), flow rate: 1.0mL / min, detection wavelength: 254nm, column temperature: 30°C, chromatographic analysis time: 100min, chromatographic conditions: methanol: water gradient elution, see Table 1 below

[0062] Table 1HPLC detects the concentration change of methanol and water gradient elution when the wampee fruit extract is detected

[0063]

[0064] Through HPLC quantitative analysis, it was found that the sum of the contents of the above-mentioned five components accounted for more than 60% of the wampee fruit extract, which were flavonoids (1). Rutin, (2). Quercetin-7-O-α -L-glucopyranoside, (3). Luteolin-4'-O-β-D-glucopyranoside, (4). Quercetin. The alkaloid compound is (5). Bantamide. The content ratio of...

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Abstract

The invention relates to a Chinese wampee fruit extract and application thereof in preparation of a medicine for preventing and treating an alzheimer disease. The prepared Chinese wampee fruit extract disclosed by the invention can be applied to the medicine for preventing and treating the alzheimer disease as a new method for preventing and treating the alzheimer disease. The medicine disclosed by the invention has the effects of resisting oxidation, inhibiting generation of Abeta, reducing activation of astrocyte cells, and protecting nerve cells, so that the cognitive disorder caused by the disease can be effectively relieved; and the Chinese wampee fruit extract has a relatively good effect on prevention and treatment of the alzheimer disease.

Description

technical field [0001] The invention relates to a new medicinal use of the extract of the wampee fruit, in particular to the preparation of the extract of the wampee fruit and its application in the preparation of drugs for the prevention and treatment of Alzheimer's disease. Background technique [0002] Alzheimer's disease (AD) is a common neurodegenerative disease and one of the most common senile dementias. Its clinical features are progressive memory loss and cognitive impairment, and its typical pathological features are It is the formation of senile plaques (Senile plaques, SP) and neurofibrillary tangles (Neurofibrillary tangles) and the damage of synapses and neurons in the affected brain areas. Beta-amyloid peptide (Aβ) is the main component of senile plaques. Studies have shown that Aβ is neurotoxic and can induce damage or even death of nerve cells. Although the neurotoxic mechanism of Aβ has not yet been fully elucidated, the oxidative stress and mitochondrial ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/75A61K31/7048A61K31/352A61P25/28
CPCA61K31/352A61K31/7048A61K36/75A61K2236/15A61K2236/33A61K2236/333A61K2236/35A61K2236/39A61K2236/51A61K2236/55
Inventor 李文德吴科锋黄韧曾小斌刘德承
Owner GUANGDONG MEDICAL UNIV
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