Probiotic composition for relieving cognitive impairment caused by long-term low-dose radiation exposure and application thereof

Abstract

The invention discloses an application of a complex microbial inoculant in preparation of a product for relieving cognitive impairment caused by radiation exposure, the active component of the complex microbial inoculant comprises a PK-R probiotic composition, and the PK-R probiotic composition contains at least one of lactobacillus rhamnosus and lactobacillus reuteri. The probiotics disclosed by the invention have a relatively good intervention effect on health hazards caused by long-term low-dose irradiation. Compared with chemical medicine intervention, the probiotic is a safe, effective, economical, convenient and practical intervention technology, is easier to accept, and provides a direct scientific basis for developing a practical low-dose radiation intervention technology.

Description

technical field

[0001] The invention belongs to the field of biomedicine, and specifically relates to a probiotic composition for alleviating cognitive impairment caused by long-term low-dose radiation exposure by regulating intestinal flora and an application thereof. Background technique

[0002] Humans are more likely to be exposed to low-level ionizing radiation than high-dose ionizing radiation. Low-dose radiation mainly comes from nuclear weapons test radioactive fallout, nuclear power plant accidents, nuclear war, nuclear pollution, radiological diagnosis and treatment, and natural radiation in areas with high background of natural radioactivity. According to the report of the International Atomic Energy Organization, the low dose is defined as: the low dose is ≤100mSv. Radiation epidemiological and clinical studies have shown that low-dose / low-dose rate ionizing radiation can induce cancer, congenital abnormalities, cardiovascular and cerebrovascular diseases, cogni...

Examples

Embodiment 1

[0027] The preparation of embodiment 1 bacterial agent

[0028] The invention provides a PK-R probiotic composition capable of alleviating cognitive impairment caused by long-term low-dose radiation exposure, including Lactobacillus rhamnosus, Lactobacillus reuteri, Bifidobacterium animalis and Bifidobacterium longum.

[0029] The present invention also provides a compound bacterial agent, the active ingredient of which is a PK-R probiotic composition, and the PK-R probiotic composition is composed of Lactobacillus rhamnosus, milk reuteri acidophilus Bacillus, Bifidobacterium animalis and Bifidobacterium longum are diluted, mixed according to the volume ratio of 4:2:2:2 to obtain the mixed solution, the mixed solution contains a concentration of 85% probiotic liquid ( active ingredient).

[0030] The total number of viable bacteria in the PK-R probiotic composition ≥ 5.0×10 8 CFU / g, among them, the number of live bacteria of a single probiotic in Lactobacillus rhamnosus and ...

Embodiment 2

[0037] The experimental mice were C57BL / 6J male mice aged 6-8w, weighing 20-25g, 30 in total, and 5 mice per cage. (The animals were conducted in accordance with the relevant provisions of the National "Regulations on the Administration of Experimental Animals", and were approved by the Experimental Animal Management and Animal Welfare Ethics Committee of the Military Medical College.)

[0038] The above 30 mice were divided into a control group, a low-dose radiation group, and a low-dose radiation+probiotic intervention group, with 10 mice in each group.

[0039] Among them, the mice in the control group were fed normally, and the animal room was alternated day and night for 12h / 12h, in a negative pressure environment with a room temperature of 20-24° C. and a humidity (40%-60%), free to eat and drink.

[0040] The low-dose radiation group was irradiated with a single 0.05Gy cobalt-60 ray on the basis of normal feeding, twice a week, 20 times in total (feeding for three month...

technical field

[0001] The invention belongs to the field of biomedicine, and specifically relates to a probiotic composition for alleviating cognitive impairment caused by long-term low-dose radiation exposure by regulating intestinal flora and an application thereof. Background technique

[0002] Humans are more likely to be exposed to low-level ionizing radiation than high-dose ionizing radiation. Low-dose radiation mainly comes from nuclear weapons test radioactive fallout, nuclear power plant accidents, nuclear war, nuclear pollution, radiological diagnosis and treatment, and natural radiation in areas with high background of natural radioactivity. According to the report of the International Atomic Energy Organization, the low dose is defined as: the low dose is ≤100mSv. Radiation epidemiological and clinical studies have shown that low-dose / low-dose rate ionizing radiation can induce cancer, congenital abnormalities, cardiovascular and cerebrovascular diseases, cogni...

Examples

Embodiment 1

[0027] The preparation of embodiment 1 bacterial agent

[0028] The invention provides a PK-R probiotic composition capable of alleviating cognitive impairment caused by long-term low-dose radiation exposure, including Lactobacillus rhamnosus, Lactobacillus reuteri, Bifidobacterium animalis and Bifidobacterium longum.

[0029] The present invention also provides a compound bacterial agent, the active ingredient of which is a PK-R probiotic composition, and the PK-R probiotic composition is composed of Lactobacillus rhamnosus, milk reuteri acidophilus Bacillus, Bifidobacterium animalis and Bifidobacterium longum are diluted, mixed according to the volume ratio of 4:2:2:2 to obtain the mixed solution, the mixed solution contains a concentration of 85% probiotic liquid ( active ingredient).

[0030] The total number of viable bacteria in the PK-R probiotic composition ≥ 5.0×10 8 CFU / g, among them, the number of live bacteria of a single probiotic in Lactobacillus rhamnosus and ...

Embodiment 2

[0037] The experimental mice were C57BL / 6J male mice aged 6-8w, weighing 20-25g, 30 in total, and 5 mice per cage. (The animals were conducted in accordance with the relevant provisions of the National "Regulations on the Administration of Experimental Animals", and were approved by the Experimental Animal Management and Animal Welfare Ethics Committee of the Military Medical College.)

[0038] The above 30 mice were divided into a control group, a low-dose radiation group, and a low-dose radiation+probiotic intervention group, with 10 mice in each group.

[0039] Among them, the mice in the control group were fed normally, and the animal room was alternated day and night for 12h / 12h, in a negative pressure environment with a room temperature of 20-24° C. and a humidity (40%-60%), free to eat and drink.

[0040] The low-dose radiation group was irradiated with a single 0.05Gy cobalt-60 ray on the basis of normal feeding, twice a week, 20 times in total (feeding for three month...

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