A kind of colloid bacillus strain and its application in seaweed degradation

A technology of Bacillus colioids and strains, applied in the field of Bacillus coliformis and its application in the degradation of seaweed, can solve problems such as complex processes, achieve the effects of improving nutritional structure, regulating body metabolism, and promoting growth

Active Publication Date: 2017-06-13
青岛藻源植物营养有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this technology is that the process is complex and requires the compounding of multiple strains

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] (1) Ascophyllum nodosum soaking

[0050] Get 50 kilograms of Ascophyllum nodosum, add water 500kg according to the ratio of Ascophyllum nodosum: water=1: 10 (weight ratio), soak 10 hours;

[0051] (2) Physical crushing

[0052] Use a grinder to crush and grind the Ascophyllum nodosum for 30 minutes;

[0053] (3) Preparation of fermentation medium

[0054] Get step (2) gained Ascophyllum nodosum homogenate 80kg, add 6kg peptone (0.6%, W / W), 1.2kg sodium chloride (0.12%, W / W), 0.7kgKH 2 PO 4 (0.07%, W / W), 0.2kgMgSO 4 (0.02%, W / W), add water to 1000L, use ammonia water to adjust the pH of the medium to 7.0;

[0055] (4) Activation of Bacillus glialis J-2 seeds

[0056] The jelly-like Bacillus J-2 (preservation number is CGMCC No.8478) was transferred from the preserved slant to a fresh test tube slant, and cultured in a constant temperature incubator at 32°C for 24 hours. Take the cultured slant, pick a full ring with an inoculation loop, inoculate it into a 250mL E...

Embodiment 2

[0062] (1) Sargassum soaking

[0063] Get 70 kilograms of sargassum, add water 1050kg according to the ratio of sargassum: water=1: 15 (weight ratio), soak 12 hours;

[0064] (2) Physical crushing

[0065] Use a grinder to crush and grind the Sargassum for 30 minutes;

[0066] (3) Preparation of fermentation medium

[0067] Get step (2) gained Sargassum homogenate 90kg, add 7kg peptone (0.7%, W / W), 1.5kg sodium chloride (0.15%, W / W), 0.6kgKH 2 PO 4 (0.06%, W / W), 0.4kgMgSO 4 (0.04%, W / W), add water to 1000L, and use ammonia water to adjust the pH of the medium to 7.5;

[0068] (4) Activation of Bacillus glialis J-2 seeds

[0069] The jelly-like Bacillus J-2 (preservation number: CGMCC No. 8478) was transferred from the preserved slant to a fresh test tube slant, and cultured in a constant temperature incubator at 30° C. for 24 hours. Take the cultured slant, pick a full ring with an inoculation loop, inoculate it into a 250ml Erlenmeyer flask filled with 50ml of sterile ...

Embodiment 3

[0074] (1) Kelp Soaking

[0075] Get 30 kilograms of kelp, add water 600kg according to the ratio of kelp: water=1: 20 (weight ratio), soak for 15 hours;

[0076] (2) Physical crushing

[0077] Use a refiner to crush and grind the kelp for 30 minutes;

[0078] (3) Preparation of fermentation medium

[0079] Get step (2) gained kelp homogenate 85kg, add 8kg peptone (0.8%, W / W), 1.4kg sodium chloride (0.14%, W / W), 0.8kgKH 2 PO 4 (0.08%, W / W), 0.3kgMgSO 4 (0.03%, W / W), add water to 1000L, and use ammonia water to adjust the pH of the medium to 7.8;

[0080] (4) Activation of Bacillus glialis J-2 seeds

[0081] The jelly-like Bacillus J-2 (preservation number: CGMCC No. 8478) was transferred from the preserved slant to a fresh test tube slant, and cultured in a constant temperature incubator at 31° C. for 24 hours. Take the cultured slant, pick a full ring with an inoculation loop, inoculate it into a 250ml Erlenmeyer flask filled with 50ml of sterile seed medium, and cultu...

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PUM

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Abstract

The present invention relates to a kind of colloidal bacillus bacterial strain, classification name is colloidal bacillus (Bacillus mucilaginosus), has been preserved on November 15, 2013 in the general microbiology center of China Committee for Microorganism Culture Preservation, and the preservation number is CGMCC No. 8478. The invention also relates to the application of the colloid bacillus in the degradation of seaweed. The colloid bacillus strain of the present invention has strong seaweed degradation ability, and the content of alginic acid in the degradation solution obtained by using the strain to degrade seaweed is ≥15g / l, which is significantly improved compared with the existing biodegradation technology. In addition, the strain also has the ability to strongly degrade cellulose, protein and alginate. The enzyme activity of cellulose in the fermentation broth is ≥48.5U / ml, the activity of protease is ≥4.5U / ml, and the activity of lyase of alginate is ≥0.855U / ml.

Description

technical field [0001] The invention relates to a colloid bacillus strain and its application in seaweed degradation, belonging to the field of biotechnology (microorganism). Background technique [0002] Seaweed (Alga) is algae that grows in the sea and is a cryptogamous plant in the plant kingdom. They are generally considered to be simple plants, and the main features are: no vascular tissue, no real differentiation of roots, stems, and leaves; no flowering, no fruits and seeds; no specialized protective tissues for reproductive organs, often directly formed by a single Cells producing spores or gametes; and no embryo formation. Seaweed is a lower plant with a wide variety. According to the different pigments contained in seaweed, it can be divided into green algae (such as Ulva), brown algae (such as kelp, sargasso, seaweed, macroalgae, etc.), Bacillus), golden algae (such as Calcifera), etc. [0003] In addition to a large amount of non-nitrogen-containing organic m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/18C12R1/07
CPCC12N1/205C12R2001/07
Inventor 李园园刘露赵宏涛鞠瑞成张鹏鹏王海鹏
Owner 青岛藻源植物营养有限公司
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