Preparation method of zinc-rich hermetia illucens larvas

A technology of black soldier flies and larvae, which is applied in the field of zinc-enriched breeding of insects, can solve the problems of large differences in inorganic zinc ion absorption and transformation capabilities, unfavorable industrial production, and large differences in zinc resistance. Improve functional value and facilitate breeding

Inactive Publication Date: 2015-03-11
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, the resistance of different yeast strains to zinc is quite different, and the ability to absorb and transform inorganic zinc ions is quite different, and the product lacks stability, which is not conducive to industrial production.
At the same time, large-scale fermentation production requires large-scale fermentation equipment investment, and the production cost is relatively high.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0016] Example 1, using wheat bran, soybean meal, rice bran, rotten fruits and vegetables, kitchen waste, etc. to prepare zinc-rich black soldier fly larvae

[0017] The obtained black soldier fly adults are placed in a 2m*2m*3m gauze, and rotten flowers, fruits and vegetables or livestock and poultry manure are placed in the gauze as the egg-inducing substrate, and the humidity is kept at 60-90%. Egg-laying box, stimulate egg-laying under light irradiation conditions; eggs collected on the same day are placed in an incubator with a temperature of 20-40°C and an air humidity of 40-90% for incubation. Add moist feed for cultivation. The above moist feed is wheat bran, soybean meal, rice bran, rotten fruits and vegetables, fresh livestock manure or kitchen waste, etc. or a mixture of two or more of them with a water content of 60 to 90%, by mixing with inorganic zinc solution. , the addition of inorganic zinc is controlled at 500mg / kg feed. One-time feeding, the stocking densi...

example 2

[0018] Example two, using fresh livestock and poultry manure to prepare zinc-rich black soldier fly larvae

[0019] The obtained black soldier fly adults are placed in a 2m*2m*3m gauze, and rotten flowers, fruits and vegetables or poultry and livestock manure are placed in the gauze as an egg-inducing substrate, and the humidity is kept at 60-90%, and at the same time, placed above the substrate. Egg-laying box, stimulate egg-laying under light irradiation conditions; eggs collected on the same day are placed in an incubator with a temperature of 20-40°C and an air humidity of 40-90% for incubation. Add moist feed for cultivation. The above wet feed is fresh livestock and poultry manure with a water content of 60-90%, which can be a single pig manure, chicken manure, cow manure, sheep manure or a mixture of two or more types of manure. Taking pig manure as an example, the content of zinc is 100-200mg / kg pig manure. One-time feeding, the stocking density of black soldier fly ...

example 3

[0020] Example 3. Determination of zinc content in black soldier fly larvae

[0021] The method for determining the content of zinc and copper in black soldier fly larvae is as follows: the collected black soldier fly larvae are first washed with deionized water, then transferred to a container with filter paper, placed in a dark place to starve for 48 hours, and changed every 12 hours during the period A filter paper to make the remaining material in the intestine of the black soldier fly larvae be completely emptied, and then freeze-dried. After grinding, accurately weigh 1.0000±0.0005g of the sample and put it into a 50ml polytetrafluoroethylene crucible. Add HF, HNO3 and HClO4 ( Volume ratio 2:1:1) solution 20ml, digested, after the digestion solution is clarified, dilute to a 50ml volumetric flask with 2mol / L HCl. The zinc content was determined by flame atomic absorption spectrometer. By applying the method of the invention, the zinc content in the black soldier fly lar...

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Abstract

The invention discloses a method for preparing zinc-rich hermetia illucens larvas. According to the method, hermetia illucens adults are first cultured; larvas are obtained after spawning and hatching; due to a large-intake characteristic of hermetia illucens larvas, the hermetia illucens larvas are used for converting a feed with added inorganic zinc, such that inorganic zinc added into the feed is converted into hermetia illucens larva bodies. The method provided by the invention is simple and effective, and is low in cost. With the method, a large amount of hermetia illucens larvas rich in protein and fat can be obtained, and zinc-rich hermetia illucens larvas can be obtained, such that hermetia illucens larva nutritive value is improved. The method has a good application prospect.

Description

technical field [0001] The invention relates to a zinc-rich culturing method for insects, in particular to a preparation method of zinc-rich black soldier fly larvae. Background technique [0002] As one of the important essential trace elements in organisms, the content of zinc in the earth's crust is about 0.004%, and its abundance in the body ranks second among metal elements, second only to iron. The content of zinc in mammals and birds is generally between 10-100 mg / kg, with an average of 30 mg / kg. The distribution of zinc in animals is unbalanced. The skeletal muscle contains the most zinc, accounting for about 50%-60% of the total zinc in the body, followed by bones, accounting for about 30% of the total. Zinc in the fur of different animal species The content changes greatly, and other tissues and organs contain less zinc. [0003] In general, the content of zinc in commonly used feeds is low, and the utilization rate is low, so animals are prone to zinc deficiency...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033A23K1/18A23K1/14A23K1/10A23K1/175
CPCA01K67/033A23K10/20A23K10/30A23K20/20A23K50/90
Inventor 张帆李燕朱建航郭孝结申高林
Owner NANCHANG UNIV
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