A method and kit suitable for detecting and identifying sensitive cotton aphids in field cotton planting

A kit and cotton aphid technology are applied in the field of cotton field pest detection and chemical control of cotton aphid, which can solve the problems of high technical requirements, strong professionalism, and time-consuming popularization and use by grass-roots agricultural technology extension departments, achieving simple application and easy production. , test the fast effect

Inactive Publication Date: 2016-06-01
XINJIANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the technical problems of the existing methods in the sensitivity determination of the existing pests to the toxicity of chemical agents, which require high technical requirements, are highly specialized, take a long time and are difficult to be widely used in grassroots agricultural technology extension departments, the present invention aims to provide a method suitable for field The method and kit for the detection and identification of sensitive cotton aphids in cotton planting not only omit the screening and sensitivity identification of sensitive cotton aphids, but also are simple and fast enough to test the toxicity sensitivity of cotton aphids to acetamiprid and imidacloprid, and to Toxicity sensitivity is graded to inform whether the drug is safe to use

Method used

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  • A method and kit suitable for detecting and identifying sensitive cotton aphids in field cotton planting
  • A method and kit suitable for detecting and identifying sensitive cotton aphids in field cotton planting
  • A method and kit suitable for detecting and identifying sensitive cotton aphids in field cotton planting

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Embodiment 1

[0024] Embodiment 1: Applicable to the method for the detection and identification of sensitive cotton aphids in field cotton planting

[0025] The sensitive strain of cotton aphid was obtained by natural selection of cotton aphid in the field and indoor selection, and the acetamiprid solution was diluted to 0.1mg.L with 0.05% TritonX-100 distilled water -1 , 0.15mg.L -1 , 0.2mg.L -1 , 0.25mg.L -1 and 0.5mg.L -1 , tested the mortality rate of the sensitive strain cotton aphid by the drug film method, and the concentration of the drug solution when obtaining its 99% mortality rate was 50mg.L respectively -1 , the set dose is 50mg.L -1 The acetamiprid solution is used as the diagnostic concentration for the test of sensitive strains of cotton aphid, and the dose of 2 times the diagnostic concentration is used as the distinguishing dose of 100 mg.L for the sensitive strain of cotton aphid in the field -1 ; The same method to obtain the differential metering of imidacloprid i...

Embodiment 2

[0026] Embodiment 2: Applicable to the method for the detection and identification of sensitive cotton aphids in field cotton planting

[0027] A specific method suitable for the detection and identification of sensitive cotton aphids in field cotton planting is as follows:

[0028] (1) According to field sampling standards, collect cotton leaves with cotton aphids and bring them back indoors.

[0029] (2) When getting the aphids, first shake off the cotton aphids on white paper in order to prevent the mechanical damage of the cotton aphids' beaks, choose the unanchored aphids with the same live wave size and put them into diagnostic test tubes, 20-30 of each tube are sealed with silica gel plugs, Seven diagnostic test tubes for each drug were replicated, and 3 blank diagnostic test tubes were used as controls.

[0030] (3) Dilute the insecticide acetamiprid and imidacloprid solution according to different metering and evenly coat the inner surface of the test tube. The glass...

Embodiment 3

[0036] Example 3: Preparation of kit for detecting and identifying sensitive cotton aphids suitable for field cotton planting

[0037] Choose a glass test tube with a length of 7cm and a diameter of 1.5cm, and the inner surface area of ​​the test tube is 27.5cm 2 Dilute the insecticides acetamiprid and imidacloprid solutions according to different metering and evenly apply them on the inner surface of the test tube. Dosage, placed at room temperature to dry, avoiding direct sunlight, and then sealed with a silica gel plug to prepare a kit suitable for the detection and identification of sensitive cotton aphids in field cotton planting.

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Abstract

The invention discloses a method and a kit applied to sensitive aphis gossypii glover detection and identification in field cotton planting. The method comprises the following steps: obtaining sensitive strain aphis gossypii glover; diluting a pesticide by 0.05% TritonX-100; obtaining reagent diagnosis concentration of 50 mg / L and 60 mg / L; taking twice dosage of the diagnosis concentration as distinguishing dosage, wherein the distinguishing dosages of acetamiprid and imidacloprid are 100 mg / L and 120 mg / L respectively; selecting a glass test tube of which the length is 7 cm, and the aperture is 1.5 cm; rolling membrane in the glass test tube to prepare a pesticide selecting kit, wherein the inner surface area of the test tube is 27.5 cm<2>, and the pesticide dosage of the tube wall is 0.44 micrograms / cm<2> and 0.53 micrograms / cm<2>; sealing the opening with silica gel to prepare the testing box. Aphis gossypii glover in cotton field is placed in during test; sensitive aphis gossypii glover screening and sensitivity identification links are eliminated; the detection is simple and quick; safety classification is conducted on toxicity and sensitivity; favorable application value is provided.

Description

technical field [0001] The invention belongs to the technical field of detection of cotton field pests. Specifically, the invention relates to a technical field of rapid indication of the sensitivity test of cotton aphids, the main pest of cotton, to the chemical insecticide acetamiprid. Further, the invention relates to a simple and rapid detection method The sensitivity of cotton aphids to the toxicity of acetamiprid, and the technical field of safe and scientific use of pesticides to control cotton aphids. Background technique [0002] Cotton aphid (Aphisgossypii Glover), commonly known as greasy insect, honey insect, oil insect, belongs to Homoptera, Aphididae, Aphid genus, is a worldwide pest, except for Tibet, it occurs all over the country, especially in the Yellow River Basin and Northwest China. The inland cotton area is more harmful. The host range of cotton aphid is wide. There are 285 species of 74 families of known host plants in the world, and 113 species have...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/50
CPCG01N33/5014
Inventor 刘小宁刘宁张学涛高希武张东海李芬马纪
Owner XINJIANG UNIVERSITY
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