Phalaenopsis blume culture medium and culture method

A technique of Phnom Penh Phalaenopsis and a cultivation method, which is applied in Phnom Penh Phalaenopsis medium and cultivation field, can solve the problems of slow growth, easy loss of Phnom Penh traits, long germination time, etc., and achieves growth promotion, strong solubility, enhanced absorption and dryness. The effect of accumulation of matter

Inactive Publication Date: 2015-03-25
TODAYS LANDSCAPING BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Based on this, it is necessary to provide a medium that can rapidly reproduce the Phalaenopsis Phalaenopsis and stably inherit the Phnom Penh traits of the leaves in order to address the problems of easy loss of Phalaenopsis traits, long germination time, and slow growth in the cultivation process of Phalaenopsis Phalaenopsis
[0005] In addition, it is necessary to provide a cultivation method that can rapidly reproduce Phalaenopsis Phalaenopsis and stably inherit the traits of Phnom Penh in the Phalaenopsis Phalaenopsis in view of the problems that the Phalaenopsis traits are easily lost, the germination time is long, and the growth is slow in the cultivation process of Phnom Penh Phalaenopsis.

Method used

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  • Phalaenopsis blume culture medium and culture method
  • Phalaenopsis blume culture medium and culture method
  • Phalaenopsis blume culture medium and culture method

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Experimental program
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Effect test

Embodiment 1

[0018] Embodiment one: prepare Phalaenopsis Phalaenopsis culture medium of the present invention

[0019] 1. According to the formula in Table 1 below, weigh each component of the MS medium.

[0020] The composition and dosage of table 1MS medium

[0021]

[0022]

[0023] 2. Preparation of primary induction medium

[0024] According to the formula in Table 2 below, each component of the Phalaenopsis Phalaenopsis primary induction medium was weighed.

[0025] Table 2 Phalaenopsis Phalaenopsis primary induction medium composition and dosage

[0026] Sample serial number

basal medium

6-BA

NAA

diethyl hexanoate

activated carbon

sucrose

agar powder

Medium No. 1

MS medium

0.6mg

0.1mg

0.01mg

0.2g

30g

7g

Medium No. 2

MS medium

0.7mg

0.12mg

0.02mg

0.2g

30g

7g

Medium No.3

MS medium

0.8mg

0.14mg

0.03mg

0.2g

30g

7g

Medium No.4

M...

Embodiment 2

[0039] Embodiment two: Phalaenopsis Phalaenopsis tissue culture method

[0040] 1. Primary induction culture

[0041] Take the pedicel axillary buds of Phalaenopsis Phalaenopsis as explants, soak them in alcohol with a mass concentration of 70-75% for 25-30 seconds, clean them with sterile water, and then use a mercuric chloride solution with a mass concentration of 0.10-0.12% Sterilize for 10 to 15 minutes, then clean it with sterile water, and then use a sterilized scalpel to cut off the incision where the pedicel axillary buds are in contact with the disinfectant at an oblique angle, and then inoculate the first-generation induced In the culture medium, make the incision of the pedicel axillary bud parallel to the surface of the culture medium, then place it in an artificial climate box, and cultivate it for 25 days under the conditions of a temperature of 23-26°C, a light intensity of 1500-2000Lux, and a light time of 12h / d , the axillary buds were germinated to obtain pr...

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PUM

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Abstract

The invention discloses a phalaenopsis blume culture medium and a culture method. The phalaenopsis blume culture medium comprises a primary induction culture medium and a proliferation subculture medium, wherein the primary induction culture medium takes an MS culture medium as a basic culture medium and is added with 6-benzylamino adenine, naphthylacetic acid, diethylaminoethyl hexanoate, active carbon, cane sugar and agar powder; the proliferation subculture medium takes the MS culture medium as the basic culture medium and is added with 6-benzylamino adenine, naphthylacetic acid, corn oligopeptide powder, coconut milk, active carbon, cane sugar and agar powder. The culture medium disclosed by the invention can be used for enabling phalaenopsis blume to be rapidly propagated, and the gold edge character of the leaves of the phalaenopsis blume can be stably inherited.

Description

technical field [0001] The invention relates to the technical field of orchid tissue culture, in particular to a Phalaenopsis Phalaenopsis culture medium and a culture method. Background technique [0002] Phalaenopsis (Phalaenopsis) is a perennial herbaceous flower belonging to the Orchidaceae Phalaenopsis. It is native to tropical and subtropical regions. Its flower is shaped like a butterfly, with bright colors and long-lasting flowering. It has high ornamental value and economic value. One of the best-selling potted plants on the Internet. The new Phalaenopsis Phalaenopsis variety, Phalaenopsis Blume, has golden-yellow leaf margins, which are both attractive for flowers and leaves. In recent years, new Phalaenopsis products with "golden edge" leaves have a large market demand, and the supply exceeds demand. The added value of the product is 3 or 4 times higher than that of ordinary varieties, and the market potential is great. [0003] Phalaenopsis Phalaenopsis is more...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈永得
Owner TODAYS LANDSCAPING BIOLOGICAL TECH
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