Phalaenopsis blume culture medium and culture method
A technique of Phnom Penh Phalaenopsis and a cultivation method, which is applied in Phnom Penh Phalaenopsis medium and cultivation field, can solve the problems of slow growth, easy loss of Phnom Penh traits, long germination time, etc., and achieves growth promotion, strong solubility, enhanced absorption and dryness. The effect of accumulation of matter
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Embodiment 1
[0018] Embodiment one: prepare Phalaenopsis Phalaenopsis culture medium of the present invention
[0019] 1. According to the formula in Table 1 below, weigh each component of the MS medium.
[0020] The composition and dosage of table 1MS medium
[0021]
[0022]
[0023] 2. Preparation of primary induction medium
[0024] According to the formula in Table 2 below, each component of the Phalaenopsis Phalaenopsis primary induction medium was weighed.
[0025] Table 2 Phalaenopsis Phalaenopsis primary induction medium composition and dosage
[0026] Sample serial number
6-BA
NAA
diethyl hexanoate
agar powder
Medium No. 1
MS medium
0.6mg
0.1mg
0.01mg
0.2g
30g
7g
Medium No. 2
MS medium
0.7mg
0.12mg
0.02mg
0.2g
30g
7g
Medium No.3
MS medium
0.8mg
0.14mg
0.03mg
0.2g
30g
7g
Medium No.4
M...
Embodiment 2
[0039] Embodiment two: Phalaenopsis Phalaenopsis tissue culture method
[0040] 1. Primary induction culture
[0041] Take the pedicel axillary buds of Phalaenopsis Phalaenopsis as explants, soak them in alcohol with a mass concentration of 70-75% for 25-30 seconds, clean them with sterile water, and then use a mercuric chloride solution with a mass concentration of 0.10-0.12% Sterilize for 10 to 15 minutes, then clean it with sterile water, and then use a sterilized scalpel to cut off the incision where the pedicel axillary buds are in contact with the disinfectant at an oblique angle, and then inoculate the first-generation induced In the culture medium, make the incision of the pedicel axillary bud parallel to the surface of the culture medium, then place it in an artificial climate box, and cultivate it for 25 days under the conditions of a temperature of 23-26°C, a light intensity of 1500-2000Lux, and a light time of 12h / d , the axillary buds were germinated to obtain pr...
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