A kind of tissue culture method of cut flower chrysanthemum sword leaf yellow

A technology of tissue culture and cut chrysanthemum, which is applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the difficulties of callus induction and rooting induction, the small share of domestic cut chrysanthemum varieties, hindering the standardization of varieties and the factory to improve product quality, improve production status, and meet product demand

Inactive Publication Date: 2017-01-11
HENAN AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
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Problems solved by technology

Due to the difficulty of callus induction and rooting induction in the tissue culture process, it seriously hinders the standardization and factory production of this variety, and the share of domestic cut chrysanthemum varieties is relatively small

Method used

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  • A kind of tissue culture method of cut flower chrysanthemum sword leaf yellow
  • A kind of tissue culture method of cut flower chrysanthemum sword leaf yellow
  • A kind of tissue culture method of cut flower chrysanthemum sword leaf yellow

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Embodiment Construction

[0019] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0020] Such as figure 1 Shown, the present invention comprises the steps:

[0021] Selection of explants: Select the young leaves or stems of cut chrysanthemums with yellow leaves or stems, which are robust and free of diseases and insect pests.

[0022] Disinfection: After rinsing the explants with streamlined tap water for 2 hours, put them in a beaker and soak them in sterile water for 2 hours; then immerse the material in 75% alcohol on a sterile ultra-clean workbench for 10 seconds and rinse with sterile water 4 times , and then transferred to 0.1% mercuric chloride solution for sterilization, leaves for 4 minutes and stems for 3 minutes; then washed with sterile water for 5 times, blotted dry with sterile filter paper, and then inoculated.

[0023] Cultivation: the cultivation phase includes leaf tissue culture and stem segment tissue culture; s...

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Abstract

The invention discloses a method for tissue culture of cut chrysanthemum chrysanthemum chrysanthemum, selecting explants: selecting young leaves or stem sections of cut chrysanthemum chrysanthemum chrysanthemum with fully expanded terminal buds, which grow vigorously and are free from diseases and insect pests; sterilize; cultivate: culturing The methods include leaf tissue culture and stem segment tissue culture; bud induction: the bud induction stage is divided into two time periods: the first period is at 25°C, with 60% light for 10 hours, and the second period is at 24°C with 0 light %, duration 10h; subculture: Inoculate the induced buds in the medium after subculture, the medium is MS+6‑BA 2mg / L+NAA 0.3mg / L; Induce rooting: seedlings. The invention uses the leaf and stem tissue of the cut chrysanthemum variety 'Jianyehuang' as explants for regenerative culture, establishes a tissue culture rapid propagation system, and meets the product demand of the cut chrysanthemum market.

Description

technical field [0001] The invention relates to a tissue culture method of cut chrysanthemum, in particular to a tissue culture method of cut chrysanthemum chrysanthemum. Background technique [0002] Chrysanthemum is the perennial root herbaceous plant of Compositae Chrysanthemum, is one of four large cut flowers in the world. Traditionally, chrysanthemum is mainly propagated by cuttings and divisions, but these two methods are easily restricted by seasons and external environmental conditions, and the reproduction coefficient is low and the quality of seedlings is poor. With the increasing quality requirements of the flower market, more and more enterprises have begun to standardize and factory-produce chrysanthemum seedlings, and tissue culture is the key technology in this production process. The cut chrysanthemum variety 'Jianyehuang' is an important domestic cut flower variety, with yellow flowers, 4-7 cm flower diameter, flowering period from August to November, and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 李永华张开明李永贺丹张凌王政雷雅凯张曼张淑梅蒋鹤
Owner HENAN AGRICULTURAL UNIVERSITY
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