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A kind of polysaccharide ester microemulsion composition

A technology of microemulsion and polysaccharide ester, which is applied in the field of biopesticide and biochemistry, can solve the problems of polymer activity reduction and effect affected by molecular weight, etc., and achieve the effects of enhancing effect, promoting plant growth, and sterilizing plant growth

Active Publication Date: 2017-12-19
成都特普生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These reports also pointed out that the effect of polysaccharides is greatly affected by molecular weight. When the degree of polymerization of monosaccharides exceeds 3, the activity of the polymer decreases significantly with the increase of degree of polymerization and molecular weight.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0017] Strain preparation: culture Trichoderma viride on the following medium: 200g potato, 20g glucose, 18-20g agar, 1000mL water, natural pH, sterilize at 121°C for 20min, inoculate, and culture at 28°C for 6 days , producing a large number of green conidia.

[0018] Liquid culture: Prepare a combination with 25g of soluble starch, 2g of calcium carbonate, 2g of potassium dihydrogen phosphate, and 5g of ammonium sulfate, add 4.0mL of corn oil, the pH is natural, add 1000mL of water to make a liquid medium, and sterilize at 121°C for 20 minutes. Use an inoculation loop to transfer the conidia of Trichoderma to a test tube containing sterile water, stir to disperse the conidia from the mycelium, filter the mycelium with sterile gauze, and adjust the concentration of the spores to 10 7 cfu / mL, inoculate into the liquid fermentation medium according to the inoculum size of 1%, after culturing at 28°C and 150r / min for 48 hours, add 10ml of butyric acid and 7g of lauric acid, stir...

Embodiment approach 2

[0020] According to the preparation ratio of 25g soluble starch, 2g calcium carbonate, 5g ammonium sulfate and 2g potassium dihydrogen phosphate, add 4.0mL soybean oil, the pH is natural, add 1000mL water to make a liquid medium, and sterilize at 121°C for 20min. Transfer the conidia of Trichoderma viride to a test tube containing sterile water with an inoculation loop according to the method of Embodiment 1, stir to disperse the conidia from the mycelia, remove the mycelium by filtration with sterile gauze, and remove the spores Concentration adjusted to 10 7 cfu / mL, inoculate into the liquid fermentation medium according to the inoculum size of 1%, add 10g of sterilized chitosan, 5ml of acetic acid, and 8g of stearic acid after cultivating at 28°C and 150r / min for 48 hours, and continue at pH2 .6 and 70r / min under stirring and cultivating for 72 hours, fully stirring evenly, filtering and collecting the reaction solution to obtain a mixed polysaccharide ester with a degree o...

Embodiment approach 3

[0022] Add 10g of chitosan to 500ml of 0.5% acetic acid aqueous solution, add 2g of dipotassium hydrogen phosphate, 5g of ammonium sulfate, 5g of sodium triphosphate and 8g of calcium dextran, stir well, add 15ml of corn oil, 5g of palmitic acid and Lipase (Novozym435) 10g, shake the flask (120 rpm) at 25°C for 15 hours, separate by liquid chromatography, the stationary phase is a C18 column (250x4.6mm), and the mobile phase is methanol: water (60:40v / v), the flow rate is 1ml / min, and the refractometer is used as a detector to collect the components in the interval of 12-25min, distill off methanol, and obtain a mixed polysaccharide ester with a polymerization degree of 100-150. The ratio of Tween 80 is added to make the polysaccharide ester microemulsion composition of the present invention.

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Abstract

The invention relates to a preparation method of a biological pesticide adopting a new biological material sugar ester as the main ingredient, and particularly relates to a sugar ester microemulsion composition, belonging to the field of biopesticide and biochemical technique. Glycan and fatty acid are subjected to reaction to generate sugar ester by microorganisms or a biological enzyme, and then the sugar ester is combined with a non-ionic surface active agent to prepare the composition for preventing and treating crop pests and promoting crop growth in a high-efficiency manner. The sugar ester microemulsion composition has positive significance on reducing dependence of agricultural production on pesticide.

Description

technical field [0001] The invention belongs to the technical fields of biopesticide and biochemistry. Background technique [0002] With the requirements of economic development and sustainable development of the environment, finding new agricultural disease control measures that meet the sustainable development of agriculture has become a hot spot in current agricultural production research. Due to the advantages of no pollution and no residue, biological pesticide products have attracted people's attention. With the development of the chemical industry and the expansion of the use of pesticides and fertilizers, the quantity and variety of chemical pesticides are increasing. Now the total output of chemical pesticides in the world (in terms of active ingredients) exceeds 3 million tons. Due to the long-term and large-scale application of chemical pesticides and chemical fertilizers, serious environmental pollution and increasing resistance of diseases and insect pests hav...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/16A01N25/04A01P7/04A01P3/00A01P21/00C12P19/04C12P19/08C12R1/885
Inventor 黄永黄瑜
Owner 成都特普生物科技股份有限公司