Composition for promoting cell regeneration and preparation method and purpose thereof

A composition and stem cell technology, applied in the direction of drug combinations, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve the problems of stem cell medical resources and high money, to improve the ability to promote cell regeneration, enhance cell regeneration The effect of the ability

Active Publication Date: 2015-03-25
林佳静
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Furthermore, stem cell therapy consumes more m

Method used

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  • Composition for promoting cell regeneration and preparation method and purpose thereof
  • Composition for promoting cell regeneration and preparation method and purpose thereof
  • Composition for promoting cell regeneration and preparation method and purpose thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment （ 1

[0041] Method for preparing human growth factor

[0042] The method comprises the following steps:

[0043] (a) Carrying out primary culture of human bone marrow mesenchymal stem cells in primary culture medium;

[0044] (b) remove the suspended cells in the primary culture medium and select the adherent cells, and subculture with the secondary culture medium; and

[0045] (c) Select the adherent cells in the subculture medium, and carry out final culture with the final culture medium. The above-mentioned final culture medium is a serum-free culture medium and contains human growth factors and / or clam extracts, so that the adherent cells can secrete various growth factors into the final culture medium to form a human growth factor solution.

[0046] In the above steps, healthy human bone marrow mesenchymal stem cells (purchased from The National Disease Research Interchange, NDRI, USA) were mixed with DMEM culture medium containing 10% fetal bovine serum (Dulbecco's modified...

Embodiment （ 2

[0054] Qualitative Analysis of Human Growth Factor Composition

[0055] Microarray chip (RayBio ＴＭ Human Cytokine Antibody Arrays) were used to detect the composition of growth factors in the dialysate, and the test results were as follows: figure 1 The human growth factor antibody microarray is shown. Depend on figure 1 (A) It can be found that the types of human growth factors contained in the composition of human growth factors prepared according to the embodiments of the present invention and their detection limits (limit of detection, LoD) are as follows: EGF (detection limit 1 pg / ml), bFGF ( Detection limit 10000pg / ml), IGF-1 (detection limit 10pg / ml), PDGF (detection limit 1000pg / ml), VEGF (detection limit 100pg / ml), TGF-β1 (detection limit 100pg / ml ), Angiogenin (detection limit 10pg / ml), IFN-γ (detection limit 100pg / ml), RANTES (detection limit 2000pg / ml), Thrombopoietin (detection limit 100pg / ml), TIMP (detection limit 1pg / ml), GRO (detection limit 100pg / ml), IL...

Embodiment （ 3

[0062] Compositions that promote cell regeneration

[0063] In another specific embodiment of the present invention, a composition for promoting cell regeneration is proposed, which at least includes the human growth factor composition according to the embodiment of the present invention, a nutritional component, an emulsifier solution, and an oil phase liquid. The nutritional ingredients mentioned above include part of the nutrient solution used in the preparation of human growth factors according to the embodiments of the present invention. In a preferred embodiment, the nutritional ingredients include at least one or more amino acids and / or vitamins. The above-mentioned emulsifier solution and oil phase liquid can embed the human growth factor composition and nutritional ingredients into a liposome embedding material in a water-in-oil-oil-in-water (W / O / W) manner.

[0064] The emulsifier solution contains about 0.5-1% Tween80, and about 1-10% sorbitan sesquioleate (Arlacel8...

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Abstract

The invention discloses a composition for promoting cell regeneration and a preparation method and use thereof. The composition for promoting cell regeneration provided by the invention is prepared by the following method: first, conducting in vitro culture of human bone marrow mesenchymal stem cells: adding a human stem cell growth factor, a granulocyte colony-stimulating factor and a clam extract, so that the human bone marrow mesenchymal stem cells can secrete a variety of human growth factors to form a human growth factor composition; and finally embedding the composition by a Water-in-Oil-in-Water (W / O / W) liposome embedding technology. The composition obtained by embedding can smear on epidermis to promote e wound healing of epidermal tissue, reduce scar formation, and activate hair follicle melanocytes and stimulate hair cell regeneration. In addition, the composition can be taken orally to promote regeneration and repair of damaged brain substantia nigra cells and hippocampal gyrus neurogenesis, and stimulate the proliferation of bone marrow hematopoietic stem cells.

Description

technical field [0001] The invention relates to a composition for promoting cell regeneration, a preparation method and application thereof. Specifically, the present invention relates to a composition for promoting cell regeneration containing human growth factor and embedded by liposome embedding technology and a preparation method thereof. Background technique [0002] The skin not only has the functions of regulating body temperature and moisture, but also the first line of defense of the human immune system. Once the protection of the epidermis is lost, it is extremely easy to be infected by foreign pathogens. Patients who have experienced burns, ulcers, inflammation, radiation therapy, surgery, and diabetes are exposed to skin defects and abnormalities for a long time, which can easily cause infection and cause great harm to the body. However, aging and disease-induced apoptosis and functional failure of internal tissues and organs, especially brain damage caused by b...

Claims

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Application Information

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IPC IPC(8): A61K38/20A61K35/618A61K9/113A61P17/02A61P17/00A61P17/14A61P17/18A61P25/28
Inventor 林佳静
Owner 林佳静
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